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Diabetes mellitus (DM) is a chronic metabolic disease with the highest rates of prevalence and mortality worldwide. Chloroxylon swietenia has been used extensively in folkloric medicine. The present study aims to determine the acute toxicity of Chloroxylon swietenia bark methanol (CSBMEt) and aqueous extracts (CSBAEt) (100, 150, 250, 500 and 1000 mg/kg body weight) and dose fixation of CSBMEt and CSBAEt in streptozotocin induced diabetic rats. Diabetes was induced in male albino wistar rats by single intraperitoneal injection of streptozotocin (50mg/kg b.w). The diabetic rats were administered with Chloroxylon swietenia bark extracts (CSBMEt and CSBAEt) (75,125 and 250mg/kg b.w) orally by intragastric intubation for 15 days. Acute toxicity studies revealed the non-toxic nature of the CSBMEt and CSBAEt. No lethality or toxic reactions found at any doses until the end of study, whereas 75 and 125 mg/kg b.w. doses of CSBMEt and CSBAEt produce no significant changes in the diabetic rats and 250mg/kg b.w. of CSBMEt and CSBAEt have significant change in the blood glucose. The results conclude that, there was no toxicity observed up to 1000mg/kg b.w. of both the extracts and 250mg/kg b.w. of CSBMEt and CSBAEt can be used as effective dose to treat diabetes.
Phenolic compounds were extracted from Litsea monopetala bark using methanol. Four fractions (I-IV) were separated from extract on a Sephadex LH-20 column using methanol as the mobile phase evaluated for their total antioxidant activity, antiradical activity against DPPH•, (2,2-diphenyl-1-picrylhydrazyl radical), and reducing power. The total phenolics and tannin contents of the fractions were determined. The highest relative content in the crude extract was found for fraction I. The content of total phenolics in fractions ranged from 169 mg/g (fraction I) to 753 mg/g (fraction II). Condensed tannins were detected in fractions II-IV. The fractions exhibited the total antioxidant activity from 1.90 mmol Trolox/g (fraction I) to 7.06 mmol Trolox/g (fraction IV). Fraction III was characterised by the highest antiradical activity against DPPH• (EC50 = 0.011 mg/assay). Fractions II and III showed the strongest reducing power which was app. four times higher than that of fractions I. The presence of three main phenolic compounds in fraction I was confirmed using RP-HPLC.
The present investigation was evaluating the potential antibacterial activity of three different extracts of the bark of Lannea coromandelica Linn. (LC) tree procured from Eastern India. Extraction of bark separation was carried out using aqueous, ethanol and a mixture of aqueous and ethanol. Microbiocides of all the extracts were separately evaluated against several microorganisms viz. Bacillus substilis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonus aeruginosa, Escherichia coli and Serratia marcescens by agar diffusion technique. The Minimum Inhibition Concentration (MIC) of all the extracts was carried out by the serial dilution method. The results of MIC ranged from 12.5 to 150 mg/ml (all the three extracts). The concentration dependent (**P < 0.01) potential antimicrobial activity was resulted and at the dose of 200 mg/ml, combined aqueous and ethanol extract of LC (LCAE + LCEE) gave significant results against gram positive bacteria where the maximum zone of inhibition was recorded against Streptococcus pyogenes (17.0± 0.05**) followed by Straphyloccus aureus (13.6 ±0.05**). Further, the same extract showed the maximum relative percentage inhibition against Straphyloccus aureus (178.64%) followed by Streptococcus pyogenes (143.42%). Such variation may be due to the effects of choice of solvent and the quantity of the extracted amount and also the geographical source of the plant part. These results represent scientific evidence to support the traditional medicinal uses of LC bark extracts and indicate a promising potential used against the treatment of infectious diseases caused by pathogenic bacteria and also provide scientific evidence for their efficacy to prepare the alternate newer medicine for antibiotics.
Preliminary phytochemical screening of various extracts of Amoora ruhituka bark were investigated which reveals the presence of several secondary metabolites in each extracts. The antibacterial activity of all the extracts was tested against four gram negative bacterial strains. The results indicated the zone of inhibition which ranges from 11.30±577 to 18.7±0.577 for different extracts in which Methanol extract has shown highest zone of inhibition for Salmonella typhimurium followed by Enterobacter aerogenes , E coli and P aeruginosa whereas benzene extract has showed the least zone of inhibition and the minimum inhibitory concentration (MIC) of the different extracts ranging from 0.78 mg/ml to 6.25mg/ml.The complete results of this study provides a essential data for the use of Amoora ruhituka for the treatment of infection associated diseases.
The antimicrobial activity of chloroform and methanol extracts of Lennea coromandelica were screened for their was studied against gram positive bacteria strains Staphylococcus aureus, Streptococcus pyogenes and Bacillus subtilis gram negative bacteria strains Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli and fungal strains such as Candida albicans, C. parapsilosis, C. krusei and C. glabrata using disc diffusion method, determination of Minimum Inhibitory Concentrations (MIC), Minimum Bacterial Concentrations (MBC) and Minimum Fungicidal Concentrations (MFC). The antimicrobial activity for different concentrations like 250 μg, 500 μg and 1000 μg of different solvent extracts of Lannea cormandelica. bacterial strains and recorded in highest mean zones of inhibition ranged from 19.6 mm and Candidal strains and the exhibited the highest mean zones of inhibition ranged from 10.6 mm. Methanol extracts showed the best results as inhibition zones against tested organisms. Results showed also that, the greatest effect was towards Staphylococcus aureus and the lowest was against Candida krusei. The present study reported the great effect of Lannea cormandelica extracts against some of most important pathogens.
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