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1

In vivo biofilm on the surface of a surgigal mesh implant

100%
Reslinski A., Mikucka A., Szmytkowski J., Gospodarek E., Dabrowiecki S.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 4
367-369
Mesh hernioplasty is among the most frequently performed surgical procedures. The introduction of mesh implants has decreased recurrence rates, but the use of synthetic materials carries the risk of infection and biofilm formation. This paper presents the course of the disease in the case of biofilm formation on the surface of an implanted surgical mesh. Antimicrobial therapy and partial removal of the implant were unsuccessful. Recurring surgical site infection could be managed only through total excision of the infected implant.
2

Biological evaluation of quaternary bis ammonium salt and cetylpyridinum bromide against S. epidermidis biofilm

100%
Stefanska J., Pietruczuk-Padzik A., Struga M., Borkowski M., Tyski S.
Polish Journal of Microbiology
|
2013
|
tom 62
|
nr 4
Quaternary ammonium compounds are broad-spectrum bacteriocides widely used as antiseptics, disinfection and preservation agents. The aim of this study was to examine the activity of two quaternary ammonium salts, cetylpyridinum bromide and a newly synthesized quaternary bis ammonium salt, against S. epidermidis biofilm. The average values of killing efficiency for cetylpyridinum bromide ranged from 26.6% to 64.1% for all tested concentrations (0.125 to 8.0 μg×mL⁻¹) and for quaternary bis ammonium salt the percentage of killing efficiency ranged from 59.7% to 88.4% for tested concentrations (from 2.0 to 128.0 μg×mL⁻¹). Both tested compounds significantly affect staphylococcal biofilms, but any of used concentrations caused a total eradication of bacterial biofilm.
3
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Electron microscopic examination of dental unit waterlines biofilm

100%
Szymanska J.
Annals of Agricultural and Environmental Medicine
|
2005
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tom 12
|
nr 2
295-298
Using the transmission electron microscope, the ultrastructural examination was conducted to detect the presence of bacterial biofilm on the inner surfaces of the tubing in dental unit waterlines (DUWL). Samples for examination were taken from the tubes providing water to high-speed and slow-speed handpieces, and to an air-water syringe before application of a disinfection procedure. The microscopic analysis made it possible to find the biofilm in all the tubes in the dental unit which were not pre-disinfected. In these samples, no significant differences were found between high-speed, slow-speed and air-water lines.
4

A case of a late and atypical knee prosthetic infection by no-biofilm producer Pasteurella multocida strain identified by pyrosequencing

100%
Romano C. L., De Vecchi E., Vassena C., Manzi G., Drago L.
Polish Journal of Microbiology
|
2013
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tom 62
|
nr 4
Prosthetic joint infections due to Pasteurella multocida are rarely but increasingly reported but no data on production of biofilm are available. We report the case of a woman with a late, haematogenous peri-prosthetic infection of cemented total knee arthroplasty caused by a strain of P. multocida identified by pyrosequencing and unable to produce biofilm. Comparison of clinical and laboratory findings with those reported in other patients evidenced differences mainly in the period of symptoms' onset and in the behaviour of some inflammatory markers.
5

Resistance of bacterial biofilms formed on stainless steel surface to disinfecting agent

100%
Krolasik J., Zakowska Z., Krepska M., Klimek L.
Polish Journal of Microbiology
|
2010
|
tom 59
|
nr 4
The natural ability of microorganisms for adhesion and biofilm formation on various surfaces is one of the factors causing the inefficiency of a disinfection agent, despite its proven activity in vitro. The aim of the study was to determine the effectiveness of disinfecting substances on bacterial biofilms formed on stainless steel surface. A universally applied disinfecting agent was used in the tests. Bacterial strains: Listeria innocua, Pseuciomonas putida. Micrococcus luteus, Staphylococcus hominis strains, were isolated from food contact surfaces, after a cleaning and disinfection process. The disinfecting agent was a commercially available acid specimen based on hydrogen peroxide and peroxyacetic acid, the substance that was designed for food industry usage. Model tests were carried out on biofilm formed on stainless steel (type 304, no 4 finish). Biofilms were recorded by electron scanning microscope. The disinfecting agent in usable concentration, 0.5% and during 10 minutes was ineffective for biofilms. The reduction of cells in biofilms was only 1-2 logarithmic cycles. The use of the agent in higher concentration - 1% for 30 minutes caused reduction of cell number by around 5 logarithmic cycles only in the case of one microorganism, M. luteus. For other types: L. innocua, P. putida, S. hominis, the requirements placed on disinfecting agents were not fulfilled. The results of experiments proved that bacterial biofilms are resistant to the disinfectant applied in its operational parameters. Disinfecting effectiveness was achieved after twofold increase of the agent's concentration.
6

Exopolysaccharide from Lactobacillus rhamnosus KL37 inhibits T cell-dependent immune response in mice

84%
Nowak B., Srottek M., Ciszek-Lenda M., Skalkowska A., Gamian A., Gorska S., Marcinkiewicz J.
Archivum Immunologiae et Therapiae Experimentalis
|
2020
|
tom 68
|
nr 3
7

Lysostaphin as a potential therapeutic agent for staphylococcal biofilm eradication

84%
Walencka E., Sadowska B., Rozalska S., Hryniewicz W., Rozalska B.
Polish Journal of Microbiology
|
2005
|
tom 54
|
nr 3
The aim was to study the activity of lysostaphin in monotherapy or in combination with oxacillin, towards biofilms built by clinical and reference S. aureus and S. epidermidis strains in the wells of microplate, in the chambers of a LabTekII chamber slide or on the polyethylene catheter. MICs of oxacillin and lysostaphin for planktonie bacteria were determined according to the standards of NCCLS. BIC (Biofilm Inhibitory Concentration) was estimated by the MTT assay. The integrity of biofilm treated with antimicrobials was also examined: by staining with FITC and laser scanning fluorescence confocal microscopy and visually by TTC reduction assay. Despite the fact that susceptibility of planktonie cultures of 25 staphylococcal strains to lysostaphin action was various, we have demonstrated the effectiveness of lysostaphin in the treatment of biofilm, built not only on the flat surface of the microplates but also on catheter's surface. The synergistic effect of subBIC lysostaphin+oxacillin was observed forMSSA and MRSA biofilms but not for 1474/01 hVISA strain. Also BICOXA for S. epidermidis RP12 and A4c strains, but not for 6756/99 MRSE biofilms was reduced when lysostaphin was simultaneously used.
8
Content available remote

Mucosal flora in Crohn’s disease and ulcerative colitis - an overview

67%
Swidsinski A., Loening-Baucke V., Herber A.
Journal of Physiology and Pharmacology. Supplement
|
2009
|
tom 60
|
nr 6
9
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The use of diode lasers in non-surgical therapy of periodontitis

67%
Kulinska-Michalska M. M., Cwiklinska N., Lisiecka M., Czaplicka-Szydlik P., Lewkowicz N.
Journal of Pre-Clinical and Clinical Research
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2020
|
tom 14
|
nr 4
10

Czego najbardziej nie lubimy i co budzi nasze największe wątpliwości w przypadku pacjentów z przewlekłym zapaleniem przewodów słuchowych

59%
Burns M. E.
Weterynaria po Dyplomie
|
2018
|
tom 19
|
nr 1
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