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The objective of this study was to investigate the effects of inoculum concentration, plant post-inoculation incubation temperature and exogenous hydrogen peroxide (H2O2) on the induction of the hypersensitive response (HR) in Nicotiana tabacum against Xanthomonas perforans. Inoculation of leaves with X. perforans at a concentration of 108 CFU · ml–1 and incubation of plants at 30°C resulted in the strongest HR elicitation. Furthermore, an exogenous supply of H2O2 accelerated X. perforans-induced HR, whereas in planta H2O2 removal by application of catalase led to a delay in HR development. Our data suggest that H2O2 has an important role in HR of N. tabacum against X. perforans.
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The effect of municipal solid waste on bacteria (Heterotrophic bacteria & Nitrobacteria) population was determine by collecting 50g sample’s of various dumpsites, put in 2 kg buckets soil and kept in the green house to mimic the natural environment for six (6) weeks. Analysis of variance (ANOVA) and least significant difference (L.S.D) were effected on the data at 5 % probability level. The analysis shows significant effect, this seem to suggest that municipal solid waste influences Heterotrophic bacteria and Nitrobacter population in soils.
The possibility of using mineral oils as a carbon source by bacteria adapted to high oil concentrations was tested in liquid media with different pH values (pH = 5,7 and 9). Two types of inocula were tested: inoculum I consisted of selected strains used in the bioremediation of oil-contaminated soils and inoculum II contained bacteria isolated from soil samples previously bioremediated at pH = 5, 7 and 9. Biodegradation was observed in all the investigated media independently of initial pH value and type of inoculum used. After 21 days of cultivation the reduction of oil content reached 60-70% in medium with pH = 5 and 80-90% in medium with pH = 7 and 9, respectively. Inoculum I consisted of strains of Arthrobacter, Pseudomonas, Agrobacter, Xanthomonas spp. After 21 days of incubation the elimination of some strains was observed. In cultures conducted at pH = 5 Agrobacter strain was no longer found, at pH 9 - the Pseudomonas strain was lost. In cultures maintained at pH = 7 all the introduced strains survived. Prolonged incubation in liquid medium at pH = 5 of strains isolated from bioremediated soils (type II inoculum) leads to the elimination of Bacillus from initial consortium of Arthrobacter, Bacillus and Pseudomonas. In cultures containing bacteria of type II inoculum (Arthrobacter, Bacillus, Achromobacter, Agrobacter, Alcaligenes, Pseudomonas, Xanthomonas, Micrococcus) conducted in liquid media at pH = 9 the Micrococcus strain was no longer present. In liquid cultures incubated at pH = 7 all introduced strains were recovered (Arthrobacter, Bacillus, Achromobacter).
The purpose of the studies conducted in the years 1996-1998 was to determine the quantitative composition of bacteria and fungi populations in the rhizosphere of spring wheat, winter wheat, potato and soybean, and in non-rhizosphere soil. Besides, the effect of root exudates of these plants on the formation of antagonistic microorganisms is presented. A microbiological analysis found out that 1 g of rhizosphere soil dry weight of the examined plants and non-rhizosphere soil contained from 4.24 x 10⁶ to 5.97 x 10⁶ bacteria colonies on average. The lowest number of bacteria was found in non-rhizosphere soil (4.24 x 10⁶ on average), and the highest in rhizosphere of potato (5.97 x 10⁶ on average). The fewest fungi colonies (28.59 x 10³ on average) were isolated from 1 g of dry weight of winter wheat rhizosphere, and the most (93.41 x 10³) from soybean rhizosphere. Antagonistic bacteria of genera Bacillus and Pseudomonas, and fungi of Gliocladium, Penicillium and Trichoderma genera dominated in winter wheat rhizosphere. Soybean roots exuded the greatest number of aminoacids (1.088 mg/ml of the solution), while spring wheat roots exuded the smallest amount (0.148 mg/ml of the solution). The percentage of aromatic and alkaline aminoacids was the lowest in potato root exudates, whilc the highest was found out in the exudates of winter wheat.
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