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Properties of avian reoviruses isolated from chickens in Poland

100%

Czekaj H., Samorek-Salamonowicz E., Kozdrun W.

Bulletin of the Veterinary Institute in Puławy

1999

tom 43

nr 1

One hundred and seventy-six samples collected from internal organs of sick birds and 40 samples taken from healthy birds were used for virus isolation. The isolation was carried out on SPF chicken embryos by inoculating them into the yolk sac with the material prepared by generally accepted methods. Three passages were performed. The presence of viral antigen was detected by the agar gel precipitation test using positive standard serum against reovirus strain S1133. Viral antigen was found in 79 samples (36.6%). Reoviruses were most often found in the bursa or Fabricius (in 61.7% of samples), liver (56.0%) and small intestine (36.8%). No virus was detected in samples collected from the caecal tonsils and trachea. The examined strains multiplied in embryo membranes and lymphatic organs of embryos inducing pathological lesions and death.

Characteristics of Polish avian reovirus strains

80%

Czekaj H., Samorek-Salamonowicz E., Kozdrun W.

Bulletin of the Veterinary Institute in Puławy

1998

tom 42

nr 2

This work aims to identify 6 virus field isolates, serologically determined as reoviruses. The type of cytopathic effect in cell cultures, physico-chemical properties and antigenic relationship were examined. The strains examined multiplied in CEF and CEK cultures, produced cytopathic effects and plaques, contained RNA in their genome, had no lipid envelope, were stable in an acid environment and belonged to the same serotype but two different subtypes.

Ograniczanie wyników

2 Bulletin of the Veterinary Institute in Puławy

2 Czekaj H.

2 Kozdrun W.

2 Samorek-Salamonowicz E.

1 1999

1 1998

Wyniki wyszukiwania

Properties of avian reoviruses isolated from chickens in Poland

Characteristics of Polish avian reovirus strains