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Growth, proline content and proline-associated gene expression of autotetraploid Betula platyphylla responding to NaHCO3 stress

100%

Mu H., Lin L., Zhang Q., Tang X., Zhang X., Cheng G.

Dendrobiology

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2016

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tom 75

Plant breeders have focused much attention on polyploid trees because of their resistance for forestry. To evaluate the impact of intraspecies genome duplication on NaHCO3 stress, a series of Betula platyphylla autotetraploids and diploids were generated from the same family. The growth, proline content and proline-associated gene expression of these autotetraploid individuals were compared with those diploid trees. Autotetraploids were superior in injury index and relative growth of height and base diameter compared to diploids. The proline content was higher in autotetraploid individuals compared to diploids. Gene expression data revealed autotetraploids were generally higher expression in BpP5CS1, BpP5CS2, Bp- P5CR1, BpP5CR2, BpP5CR3 and BpOAT and were lower expression in BpProDH and BpP5CDH compared to diploid trees. These results shed light on resistance variation in birch autotetraploidization and polyploidy breeding as a new approach for genetic improvement of birch trees.

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Production of intergeneric allotetraploid between autotetraploid non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) and autotetraploid radish (Raphanus sativus L.)

100%

Sun C.-Z., Li Y., Zhang S.-N., Zheng J.-S.

Acta Societatis Botanicorum Poloniae

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2014

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tom 83

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nr 1

Intergeneric hybrids between non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino; 2n = 4x = 40) and radish (Raphanus sativus L.; 2n = 4x = 36) were obtained through ovary culture and embryo rescue. Some hybrid embryos (0.11 per ovary) were produced, but only 4 of them germinated. As most hybrid embryos failed to develop into plantlets directly, plants were regenerated by inducing shoots on the cultured cotyledon and inducing roots on the root induction medium. All hybrid plants were morphologically uniform. They resembled the non-heading Chinese cabbage in the long-lived habit, the plant status, the vernalization requirement and the petiole color, while the petiole shape, leaf venation pattern and flowers were more similar to those of radish. Upon examination of the flowers, these were found to have normal pistil, but rudimentary anthers with non-functional pollen grains. The somatic chromosome number of F1 plants was 38. Analysis of SSR banding patterns provided additional confirmation of hybridity.

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Fertility improvement in pea [Pisum sativum L.] autotetraploids - mutation breeding

100%

Kumar H., Mercykuty V. C., Srivastava C. P.

Journal of Applied Genetics

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1995

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tom 36

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nr 1

C₂ seeds of autotetraploids (colchicine-induced) of two diverse genotypes, T 163 and 5064-S, of pea (Pisum sativum L.) and their corresponding diploids were irradiated with 10 kR gamma-ray. Autotetraploids showed a high seed-sterility as compared to diploids. Seed fertility increased in M₂ as compared to M₁. Both quadrivalent and bivalent frequencies decreased in the M₁ generation of autotetraploids in relation to their respective controls. However, a positive shift in the mean bivalent formation was noted at the cost of other configurations in C₃-M₂ with respect to C₂-M₁ and varied with the genotype. In the M₂ generation of autotetraploids, the variability was relatively higher for the number of pods per plant and seed yield per plant. Although the mean values for pods per plant, number of seeds per pod and seed yield per plant remained more or less parallel in autotetraploids in M₁ and M₂ generations, there was an increase in the upper range limit probably due to micromutation for these characters in M₂.

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Tissue-culture-responsive and autotetraploidy-responsive changes in metabolic profiles of cucumber [Cucumis sativus L.]

80%

Filipecki M., Yin Z., Wisniewska A., Smiech M., Malinowski R., Malepszy S.

Journal of Applied Genetics

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2006

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tom 47

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nr 1

Somaclonal variation commonly occurs during in vitro plant regeneration and may introduce unintended changes in numerous plant characters. In order to assess the range of tissue-culture-responsive changes on the biochemical level, the metabolic profiles of diploid and tetraploid cucumber R₁ plants regenerated from leaf-derived callus were determined. Gas chromatography and mass spectrometry were used for monitoring of 48 metabolites and many significant changes were found in metabolic profiles of these plants as compared to a seed-derived control. Most of the changes were common to diploids and tetraploids and were effects of tissue culture. However, tetraploids showed quantitative changes in 14 metabolites, as compared to regenerated diploids. These changes include increases in serine, glucose-6P, fructose-6P, oleic acid and shikimic acid levels. Basing on this study we conclude that the variation in metabolic profiles does not correlate directly with the range of genome changes in tetraploids.

Ograniczanie wyników

2 Journal of Applied Genetics

1 Acta Societatis Botanicorum Poloniae

1 Dendrobiology

1 Cheng G.

1 Filipecki M.

1 Kumar H.

1 Li Y.

1 Lin L.

1 Malepszy S.

1 Malinowski R.

1 Mercykuty V. C.

1 Mu H.

1 Smiech M.

1 Srivastava C. P.

1 Sun C.-Z.

1 Tang X.

1 Wisniewska A.

1 Yin Z.

1 Zhang Q.

1 Zhang S.-N.

1 Zhang X.

1 Zheng J.-S.

1 2016

1 2014

1 2006

1 1995

Growth, proline content and proline-associated gene expression of autotetraploid Betula platyphylla responding to NaHCO3 stress

Production of intergeneric allotetraploid between autotetraploid non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) and autotetraploid radish (Raphanus sativus L.)

Fertility improvement in pea [Pisum sativum L.] autotetraploids - mutation breeding

Tissue-culture-responsive and autotetraploidy-responsive changes in metabolic profiles of cucumber [Cucumis sativus L.]