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Molecular characterization of atrazine resistance in common ragweed [Ambrosia artemisiifolia L.]

100%

Cseh A., Cernak I., Taller J.

Journal of Applied Genetics

2009

tom 50

nr 4

321-327

Common ragweed (Ambrosia artemisiifolia L.) is the most frequent weed in the Carpathian Basin and is spreading fast in other parts of Europe. In recent years, besides the wild type, a mutant genotype resistant to atrazine herbicides has evolved and is now widespread in many areas. The present study demonstrates that the atrazine resistance of ragweed is maternally inherited, and is caused by a point mutation in the psbA chloroplast gene. The promoter 5'-untranslated region and the open reading frame regions of the gene were analysed, and a homology search was performed. Both the atrazine-resistant and susceptible types of cpDNA were present in atrazine-resistant plants, while the mixed presence of both genotypes in the same plant, known as heteroplasmy, was not unequivocally detectable in susceptible plants.

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Polyamines as a potential chemotaxons of resistant and susceptible biotypes of Chenopodium album to atrazine

67%

Giebel J., Stachecki S., Praczyk T.

Journal of Plant Protection Research

2000

tom 40

nr 3-4

The level of free (PAs) and conjugated (CPAs) soluble polyamines in leaves of Chenopodium album was determined using the fluorometric method for dansylated derivatives. The tests made at the 4-6 leaf growth stage showed that the concentration of PAs in susceptible (S) and resistant (R) biotypes ranged between 32-43 μg and 45-56 μg per 100 μI of cell sap, respectively. The analyses performed later, namely at the beginning of flowering showed that the difference in the level of polyamines between S and R biotypes was greater. In this case the amount of PAs in biotype S ranged between 15-34 μg and in biotype R between 51-73 μg per 100 μI of cell sap. Therefore, the level of PAs cans act as an indicator for susceptibility or resistance of C. album biotypes to atrazine.

Ograniczanie wyników

1 Journal of Applied Genetics

1 Journal of Plant Protection Research

1 Cernak I.

1 Cseh A.

1 Giebel J.

1 Praczyk T.

1 Stachecki S.

1 Taller J.

1 2009

1 2000

Wyniki wyszukiwania

Molecular characterization of atrazine resistance in common ragweed [Ambrosia artemisiifolia L.]

Polyamines as a potential chemotaxons of resistant and susceptible biotypes of Chenopodium album to atrazine