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Solute-free water retention in preascitic cirrhotic rats following intravenous water loading

100%

Sansoe G., Aragno M., Smedile A., Rizzetto M., Rosina F.

Journal of Physiology and Pharmacology

2009

tom 60

nr 4

111-117

Increased extracellular fluid volume (ECF) characterizes compensated cirrhosis. To identify the mechanisms of fluid retention in cirrhosis through clearance methods, 10 control and 10 preascitic rats with CCl4-induced cirrhosis were studied following i.v. loading with 1 ml 5% glucose solution. Glomerular filtration rate and renal plasma flow were evaluated through inulin and para-aminohippurate clearances; water and electrolyte handling was assessed measuring urine and plasma osmolarity, electrolyte excretions, and tubular solute-free water reabsorption (TFWR = osmolar clearance minus urinary output); ECF was assessed through hormonal status determination. After water loading, cirrhotic rats had increased ECF (lower plasma renin activity and aldosterone and higher atrial natriuretic peptide levels, all P<0.03), solute-free water retention (increased TFWR and decreased plasma osmolarity, all P<0.05), reduced absolute and fractional sodium excretions (P<0.05). Cirrhotic rats showed sodium retention in the medullary thick ascending limb of Henle’s loop (i.e. increased values of TFWR for any given value of osmolar clearance). Trans-tubular potassium gradient in medullary collecting duct was similar in the two groups (P=0.55), ruling out aldosterone-dependent sodium retention and potassium hyper-secretion. In experimental preascitic cirrhosis NaCl retention in the ascending limb of Henle’s loop increases medullary interstitial tonicity leading to vasopressin-independent water back-diffusion in thin descending limb of Henle’s loop and collecting duct.

Dynamics of sodium retention in preascitic cirrhotic rats assessed through parathyroid hormone injection

100%

Sansoe G., Mastrocola R., Aragno M., Parola M.

Journal of Physiology and Pharmacology

2014

tom 65

nr 5

Characteristics of turkey breast meat showing different signs of bursitis and ascites

84%

Januskeviciene G., Paulauskas V., Milius J., Zaborskiene G., Garmiene G., Stankiene J.

Bulletin of the Veterinary Institute in Puławy

2010

tom 54

nr 4

The amounts of biogenic amines (putrescine, histamine, cadaverine, tyramine, spermidine, and spermine), lactic acid, pH, and number of Enterobacteriaceae, Escherichia coli, and coagulase producing Staphylococci were determined in healthy turkeys and in the fillets of turkey breasts with the signs of ascites and bursitis. The examination of the amines was performed by high performance liquid chromatography; the amount of lactic acid was determined by capillary isotachophoresis method; pH value and microbiological indicators - by standardised methods. All determinations were done after 24, 72, and 120 h after slaughter of the turkeys. The fillets of the breasts were stored in the refrigerator at 4°C. The significant differences in the total amounts of biogenic amines in carcasses of healthy turkeys and carcasses of turkeys with substantial lesions of bursitis and medium or substantial lesions of ascites were determined within 5 d after slaughter (P<0.00l). The amounts of biogenic amines in the breast fillets of turkeys with low lesions of ascites and bursitis were very similar to that of the control group during the whole period of maturation. The average amount of lactic acid throughout 5 d after slaughter increased only in the breast fillets of healthy turkey (maximum value was 1.032 mg/100 g) and insignificantly increased in the carcasses with low lesions of ascites and bursitis (maximum value was 0.983 mg/100 g). The results of investigation indicate the breast fillets of turkeys with medium and substantial lesions of ascites and substantial lesions of bursitis are not fit for human consumption. However, in the cases of low lesions of ascites and low and medium lesions of bursitis, the fillets are not fit for maturation, but could be thermally processed in the period of 24 h after slaughter.

Ograniczanie wyników

2 Journal of Physiology and Pharmacology

1 Bulletin of the Veterinary Institute in Puławy

2 Aragno M.

2 Sansoe G.

1 Garmiene G.

1 Januskeviciene G.

1 Mastrocola R.

1 Milius J.

1 Parola M.

1 Paulauskas V.

1 Rizzetto M.

1 Rosina F.

1 Smedile A.

1 Stankiene J.

1 Zaborskiene G.

1 2014

1 2010

1 2009

Wyniki wyszukiwania

Solute-free water retention in preascitic cirrhotic rats following intravenous water loading

Dynamics of sodium retention in preascitic cirrhotic rats assessed through parathyroid hormone injection

Characteristics of turkey breast meat showing different signs of bursitis and ascites