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In the year 2003, the occurrence of arbuscular mycorrhizal fungi (AMF) of the phylum Glomeromycota in cultivated and uncultivated soils of the Lubuskie province was investigated. The occurrence of AMF was examined based on 56 root and rhizosphere soils collected under 7 species of cultivated and uncultivated plants growing in 28 localities. Spores of AMF were isolated from both field-collected samples and trap cultures. They were revealed in 100% of field soils and 93.8% of trap cultures and represented 7 of the 8 recognized genera of the Glomeromycota. The arbuscular fungi occurring distinctly more frequently in the soil and root samples examined were members of the genus Glomus. The species of AMF most frequently occurring in cultivated soils of the Lubuskie province were G. claroideum, G. constrictum, G. deserticola and G. mosseae, whereas G. claroideum, G. constrictum, G. deserticola, G. mosseae, and S. dipurpurescens were more frequently found in uncultivated sites. The analysis of similarity of the species composition of AMF populations in sites of the Lubuskie province and the Western Pomeranian province earlier examined showed that (1) the occurrence in Poland of most taxa of these fungi detected in the study presented here is even and does not change with time, (2) the communities of AMF area are stable, despite the arduousness resulting from the agricultural and chemical practices conducted, and (3) the species diversity of the plants cultivated in a long period of time has no influence on the species composition of populations of AMF.
The ontogenetic development and morphological properties of spores of two species of arbuscular mycorrhizal fungi (Glomeromycota) of the genus Glomus, G. claroideum and G. spurcum, are described and illustrated. Spores of the two species were not earlier found in Poland, and this paper is the first report of the occurrence of G. spurcum in Europe. In one-species pot cultures with Plantago lanceolata as the host plant, the mycorrhizae of G. claroideum consist of arbuscules, vesicles, as well as intra- and extraradical hyphae staining intensively with trypan blue. Glomus spurcum mycorrhizae were not recognized, because many attempts to establish one-species cultures of this fungus failed. Additionally, the distribution of both the fungi in the world is presented.
In 1995-1998 two field experiments were carried out on a sandy soil. Supplemental irrigation and mineral fertilization modified the yield of pea and white lupine. Higher yield increases gave pea. Furthermore, the applied practices influenced both the anatomical and morphological features of both plant species. Leaves, of irrigated and well fertilized plants were bigger and had an increased number of stomata of larger dimensions. Leaves of both plant species harboured numerous populations of spores of microscopic fungi. More abundant spore populations of these fungi were isolated from the white lupine leaves. The most frequently revealed spores were those of the genera Cladosporium and Uromyces. In white lupine the abundance of spores associated with leaves increased due to the irrigation applied, whereas in pea following the use of fertilizers. Additionally, irrigation increased the number of spore s of arbuscular fungi of the genus Glomus the root zone of pea, but decreased their occurrence among roots of white lupine.
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