The goal of the study was to evaluate the accumulation of aquaporin mRNA in Sium latifolium leaves at the different developmental stages under moderate water deficit. The accumulation of aquaporin mRNA in leaves of two ecotypes of Sium latifolium: air-aquatic (control) and terrestrial ecotype (affected by moderate water deficit) was studied using RT-PCR analysis and degenerate primers complementary to conservative motifs of the majority of dicotyledons PIP aquaporin mRNA. It was concluded that moderate water deficit has a strong impact on the expression of S. latifolium PIP aquaporins.
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