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The accumulation of elements in biocenoses of aquatic ecosystems depends primarily on the forms of elements in the environment. The bioaccumulation coefficient (BC) is a measure of the intensity of an elements uptake of trace by living organisms. Manganese is an essential element for both plant and animal organisms. However, its excess may cause a toxic effect, i.e. it disturbs the activity of synapses, thus leading to an impaired functioning of the central nervous system. This study focused on the accumulation of manganese in individual links of an aquatic ecosystem food chain under conditions of extensive carp farming. The BC values were computed and the pollution degree of the fish pond was estimated. The investigations were conducted in a fish pond situated in Mydlniki and fed with water from the Rudawa River. Water, bottom sediment, benthic organisms (Diptera Chironomidae larvae) and carps were sampled from the pond. Organs most strongly involved in the metal metabolism (gills, gonads, liver and muscles) were prepared from sampled carps. Manganese concentrations were determined in all samples using atomic emission spectroscopy after wet mineralization of samples in a closed system in a microwave mineralizer. The concentrations of manganese in the abiotic elements of pond ecosystems were low and should not pose any threat of its excessive accumulation in living organisms. However, the manganese concentrations in the benthic organisms and in the analyzed carp organs were high. Similarly, other authors found high manganese concentration in fish living in the environments polluted with this element. The value of manganese enrichment coefficient for the bottom sediments in relation to its water concentration was high. The biggest manganese content was assessed in gills, then in the liver and gonads, and the smallest one - in carp muscles. Manganese BCs in the gills of carps in relation to its content in water and bottom sediments were 176.6 and 0.08, respectively. The BC values in relation to the manganese concentration in water were much higher, but lower in comparison to its content in bottom sediments than reported elsewhere. This confirms that the BC for manganese in gills varies depending on the water pollution level.
The possibility of using sturgeon as a stock component during the rearing of pike (Esox lucius L.) fry in tanks was determined. The experiment was conducted in two stages. In the first, pike fry with a body weight of 0.1 g were reared in monoculture and polyculture with a sturgeon component comprising 40 and 80% of the pike biomass. In the second phase, pike fry weighing an average of 3 g were reared in monoculture and polyculture with a sturgeon component comprising 10 and 20% of the pike biomass. The introduction of the sturgeon improved feed utilization and lowered feed conversion ratios from 1.4 to 0.8 in the older pike group (statistically significant difference at P < 0.05). In experiment stage II, the pike fry reared in polyculture with sturgeon attained statistically significant (P < 0.05) higher body growth in comparison with the monoculture variant. Pike survival was the lowest in the monoculture at 72.1 and 91.4% in stages I and II of the experiment, respectively. With regard to the smaller fry, this was due primarily to cannibalism with such losses comprising over 50% of the total losses throughout rearing. Survival in the polycultures was as much as 12% higher. The authors believe that the possibility of rearing these two species together stems from their different behavior and feeding strategy. Another benefit was that the labor-intensive removal of feed not consumed by the pike fry was lowered which meant that there was minimal interference in the tank during rearing.
Alien species are now found all over the world. New fish parasites have been unintentionally introduced with infected alien fish imported for aquaculture or have sometimes spread with their intermediate invertebrate hosts transported in the ballast waters of ships. Four alien fish parasites have been recorded in Polish coastal lakes and lagoons, all parasitising eels. Three were introduced with the final host – the Japanese eel – introduced for aquaculture (Anguillicola crassus, Pseudodactylogyrus anguillae and Pseudodactylogyrus bini) and one (Paratenuisentis ambiguus) with its sole intermediate host (Gammarus tigrinus).
In this study, the comparative effects of the two disturbances (aquaculture and water level fluctuations) on macroinvertebrate communities were explored in two waterbodies connected with the reservoir system of Three Gorges Reservoir (TGR) in China. One water body called Gaoyang Bay which suffered organic pollution from intensive aquaculture. Another named Hanfeng Lake, where the effects of water-level fluctuations are obvious. The results showed that aquaculture could significantly affect the chemical forms of nutrients, decrease the α-diversity and increase the β-diversity of macroinvertebrates although the communities in the treatment area in Gaoyang Bay were not fundamentally changed comparing to the control area in the same bay. The densities of macroinvertebrates in the treatment area were significantly lower than that of the control area. The composition of functional feeding groups in the treatment area was close to that in Hanfeng Lake, but obviously different from that in the control area in Gaoyang Bay and the collectors and predators dominated the communities in this control area with the highest percentages. Although water-level fluctuations had negative effects on the communities by decreasing the α-diversity and increasing the β-diversity, which were confirmed by the values of Simpson index, Shannon-Wiener index and Harrison's index in the control area of Gaoyang bay and Hanfeng Lake, the adverse effects were relative low compared to aquaculture. In the bays of TGR, the small-scale disturbance (aquaculture activities) had more significant negative effects compared to the big-scale disturbance (water-level fluctuations related to dam operation of TGR).
Background. The purpose of this study was to determine the timing of gonadal differentiation in two gadoids: Atlantic cod, Gadus morhua L., and haddock, Melanogrammus aeglefinus (L.). This information is required to develop a practical protocol for the production of monosex populations of these species for aquaculture. Materials and Methods. Cultured larvae and juveniles were collected weekly, measured (total length; TL), prepared histologically and then examined microscopically for the presence of characteristic stages of gonadal differentiation. Results. In Atlantic cod, undifferentiated gonads were present by 18 mm TL, at 84 days post hatch (dph), and definitive germ cells by 19 mm TL (90 dph). Ovarian cavities were first observed at 27 mm TL (102 dph), and by 35 mm TL (112 dph) anatomical divergence into two types of gonads was clear. In haddock, undifferentiated gonads were observed at 21 mm TL (64 dph) and an ovarian cavity was evident at 29 mm TL (71 dph). Conclusion. Gonadal differentiation in Atlantic cod and haddock occurs at roughly the same size in both species (27 and 29 mm TL, respectively). For successful sex reversal, the administration of steroids should therefore begin at approximately 25 mm TL, shortly after weaning onto dry feed.
In this study, it was aimed to determine the effects of zeolite and bentonite on the ammonium adsorption at different temperatures. In this research three trial groups with 3 repetitions were created for three different water temperatures (18±0.1°C, 24±0.0°C, 27±0.0°C). Experimental groups were prepared by adding NH4 + amount of 10.5 mg/l in 2 liters of water. After that, zeolite, zeolite+bentonite and bentonite were added into the bottles as 10 gram per liter. Water temperature, pH and TAN (Total Ammonium Nitrogen) values were determined during the trial period. At the end of trial TAN values at 27 °C were recorded as 10.103±0.11 mg/l, 9.227±0.13 mg/l and 7.933±0.17 mg/l in zeolite, zeolite+bentonite and bentonite groups, respectively. At the end of trial TAN values at 24 °C were recorded as 10.027±0.17 mg/l, 9.282±0.15 mg/l and 8.336±0.15 mg/l in zeolite, zeolite+bentonite and bentonite groups, respectively. At the end of trial TAN values at 18 °C were recorded as 9.012±0.28 mg/l, 7.702±0.14 mg/l and 6.594±0.14 mg/l in zeolite, zeolite+bentonite and bentonite groups, respectively. Maximum ammonium removal capacity, qe, was found to be 0.50 mg/g in the bentonite (18 °C). The TAN values determined at 18 °C were statistically more significant (p<0.05) than the TAN values obtained at 24 °C and 27 °C.
The results of using the waste water from recirculating aquaculture system for phytoand zooplankton cultivation are presented in the article. The physico-chemical parameters of the aquaculture system’s waste water and comparative culture media were recorded during hydrobionts cultivation. The indicators of productivity and the biochemical composition of investigated cultures were determined. The biochemical analysis of studied cultures included the investigations of the content of total proteins, lipids, carbohydrates, carotenoids and photosynthetic pigments. Using aquaculture system’s waste water as a medium for the cultivation of phyto- and zooplankton can significantly reduce the cost of technology for live feed biomass increase in the aquaculture industries.
Inducing protection against bacterial diseases in fish culture through the application of specific vaccines is one of the most important prevention methods. However, some immunization techniques actually used under hatchery conditions are not as effective as they should be. Therefore, current research is focusing on how to improve the potency and efficacy of the antigens and how to optimally activate the immune response. In our preliminary study, we determined the influence of the anaesthetic Propiscin (IFI, Poland) on the effectiveness of a vaccine applied by intraperitoneal injection or immersion to rainbow trout (Oncorhynchus mykiss). The fish were vaccinated via injection or immersion with an anti-Yersinia ruckeri vaccine (Sanofi, France) under Propiscin-induced anaesthesia or without it. On day 21 after vaccination, all the fish groups were challenged with live Y. ruckeri bacteria (0.2 ml of bacteria at 1 x 10⁶ ml⁻¹ ). Control groups (only anaesthetized and injected PBS or immersed) were infected but not immunized by vaccine. The blood and pronephros were separated before vaccination (10 fish), 21 days after vaccination (10 fish) and 21 days after the challenge (10 fish). ELISPOT assays for the quantification of total immunoglobulin secreting cell (ISC) and specific antibody secreting cell levels (ASC) were used. The total Ig levels in the serum were also measured by spectrophotometric assay and the titers of anti-Y. ruckeri antibody were measured by the micro-agglutination method. Mortalities were collected and tabulated and the presence of pathogen was confirmed by isolation from fish kidneys. The results of this study showed that Propiscin applied at vaccination time increased the nonspecific and specific immune response and protective effectiveness of vaccine after the challenge with pathogenic bacteria. The highest level of total ISC and specific ASC in fish vaccinated by immersion and injections under anaesthesia were observed. The highest total Ig levels and titers of specific antibody in fish vaccinated under anaesthesia were also observed. Cumulative mortality rates were lowest in the fish vaccinated by injection (5%) and immersion (10%) under anaesthesia as compared with fish vaccinated by injection (20%) and immersion (35%) without anaesthesia. Cumulative mortality in the control, non-vaccinated group of fish was 80%.
An increase in feeding frequency and stock density generally enhanced total production. Maximum final mean body weight was recorded in fish fed once in 2 days at a density of 1 fish per 5 dm3. Fish fed once in 2 days exhibited the maximum conversion rate and efficiency and this may be considered as the optimum feeding for M. keletius. An increase in density beyond 1 fish per 5 dm3 significantly reduced all food utilization parameters except absorption efficiency. The adverse effects of high density could be compensated by increasing the feeding schedule.
A 21-day feeding experiment was conducted to assess the effects of diets supplemented with fish intestinal bacteria producing extracellular enzyme on growth and survival of rohu, Labeo rohita (Hamilton) spawn (average weight 0.3 mg). Intestinal bacteria were isolated from healthy rohu fingerlings (average weight 3.51 ±0.31 g). Six isocaloric (4.24 kcalּg-1) and isonitrogenous (approximately 35% crude protein) diets (D1-D6) were prepared containing 32% fish meal, 34% mustard oil cake, 30% rice bran, 2% cod liver oil, and 2% vitamin premix. Experimental diets (D2-D6) were supplemented with the isolated bacterial strain Bacillus circulans Lr 1.1 at five different levels (4 × 105, 4 × 106, 4 × 107, 4 × 108 and 4 × 1010 cells per 100 g of feed, respectively). Rearing of spawn on diet D5 (supplemented with 4 × 108 cells per 100 g) resulted in better growth and survival (97.33 ±1.15%) of rohu spawn compared to those with the control diet (diet D1, without any microflora supplementation) and other experimental diets. Diet D5 resulted in significantly higher growth and specific growth rate (SGR) followed by diets D6 and D4. RNA and DNA contents in the spawn were measured as an index of growth. The correlation coefficient (r value) of 0.831 and regression line having a slope of 0.79 suggested a significant positive correlation (0.01 level) between RNA-DNA ratio and specific growth rate.
A laboratory feeding trial was conducted for 80 days using rohu, Labeo rohita, fingerlings (5.67 ±0.25 g) to evaluate the optimum dietary carbohydrate level. Five semipurified diets containing 30, 35, 40, 45, and 50% carbohydrate (diets D1 to D5) were formulated and fed to triplicate groups of fish. All the diets were isonitrogenous (35% protein) and almost isocaloric. Fish performance in terms of average live weight gain (%), SGR (%/day) and FCR was best with 40% carbohydrate level. Significantly (P < 0.05) poor growth of fish also was noticed in the groups reared on diets with 30 and 35% levels of dietary carbohydrate, however, no significant difference in PER and ANPU (%) was obtained with diets D2 and D3. Although no significant difference in apparent protein digestibility was recorded up to 45% dietary carbohydrate level, lipid, ash, dry matter, and energy digestibilities were found to be significantly (P < 0.05) higher for the diets D2 and D3. These two dietary treatments also resulted in higher deposition of protein and lipid and lower moisture and ash contents in the fish muscle. Amylolytic and proteolytic enzyme activities also were estimated to be higher in the groups of fish fed 35% and 40% carbohydrate diets. The study indicated that a minimum level of 40% dietary carbohydrate is required (protein content being 35%) for optimum growth, feed conversion and nutrient utilisation in rohu, Labeo rohita, fingerlings.
Rainbow trout (10-20 g body weight) from a local fish farm were examined after low levels of mortality at a temperature of 10°C were observed. Dystrophic changes were observed only in the gills. Based on its unusual clinical presentation, this condition was termed atypical bacterial gill disease (ABGD). Only Flavobacterium psychrophilum was isolated from the gills. On the day the first symptoms of the disease appeared and on the third, seventh, and tenth days afterwards blood from 20 afflicted fish (diseased) and 20 unafflicted fish was drawn to examine the nonspecific humoral defense mechanism. In the present immunological studies a strong immunosuppression effect was observed in fish with symptoms of atypical bacterial gill disease. The results showed that serum lysozyme activity and Ig levels were statistically (P < 0.05) lower in fish with disease symptoms in comparison with control, disease-frie fish. The activity of serum ceruloplasmine was very high in fish with disease symptoms in comparison with the control fish. The results indicated that the suppression occurred as atypical bacterial gill disease was developing. All immunological parameters were statistically significantly lower after three, seven, and ten days of disease symptoms in comparison with the disease-free, control fish. This preliminary study indicated that ABGD with Flavobacterium psychrophilum has a strong suppression effect on the nonspecific humoral defense mechanisms.
Five combinations of human chorionic gonadotropin (hCG) dose and five latency periods were evaluated to observe the pattern of deformed larvae among the hatchlings in walking catfish, Clarias batrachus (L.). A higher percentage (7%–12%) of deformed larvae were observed at 1000, 2000, and 5000 IU · kg-1 with 14–23 h latency combinations. The results of the study indicated that 3000 IU · kg-1 with 14–23 h and 4000 IU · kg-1 with 14–17 h latency combinations were preferable to reduce the deformed larvae (4%–6%) among the hatchlings during induced spawning of the catfish.
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