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The preliminary studies were conducted in the years 2006-2008 at the Fruit Experimental Station of Wrocław University of Environmental and Life Sciences. One year old trees of Harcot, Hargrand and Bergeron cultivars grafted on Pumiselect® vegetative rootstock were planted in spring 2006, at a spacing of 4.0 x 3.2 m (780 trees∙ha-1). The experiment was established in a randomized block design in 4 replications with 3 (ʻBergeronʼ) or 4 (‘Harcot’, ‘Hargrand’) trees per plot. The trees of all estimated cultivars started cropping in the third year after planting but the obtained yields were not too high. ‘Harcot’ and ‘Bergeron’ produced only some fruits. The significantly highest crop per tree and largest fruit were recorded for ‘Hargrand’. ‘Bergeron’ trees produced clearly the smallest sized apricots. ‘Harcot’ proved to be the most vigorous cultivar. The weakest growth was noted for ‘Bergeron’. The highest per cent of dead trees was observed for ‘Hargrand’, the lowest one for ‘Harcot’. However ‘Harcot’ showed to be very susceptible to bark and wood diseases. ‘Bergeron’ fruits were very susceptible to peach scab.
In the field experiment, 5-year-old 'Morden 604' and 'Miodowa' apricot trees grafted on the seedling rootstocks Wangenheim Prune and Erunosid (both P. domestica L.), as well as Polish selection apricot (P. armeniaca L.) geno­types A4 and M46 were compared with trees of the same cultivars on the standard P. divaricata rootstock. Vigour in apricot trees was affected by rootstock type. Trees of both cultivars on P. divaricata and 'M46' had the largest trunk cross-sectional area. The most dwarfing rootstock was 'Wangenheim Prune'. Rootstock had no effect on the fruiting of 'Morden 604' trees. In the case of the Miodowa cultivar, the highest cumulative yields were harvested from trees grafted on P. divaricata and 'M46', while the lowest from those on 'Erunosid'. The results revealed that 'Wangenheim Prune', in compari­son to other rootstock tested, significantly reduced the mean fruit weight. In addition, trees grafted on this rootstock had the highest mortality. Mean content of soluble solids in fruits of 'Morden 604' and 'Miodowa' apricots was not affected by root- stocks in this study. Generally, the value of A4 and M46 genotypes used as seedling rootstocks for apricot trees in this experiment was higher than the P. divaricata stan­dard rootstock.
Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.
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