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Most flowering plants, including important crops, require double fertilization to form an embryo and endosperm, which nourishes it. Independence from fertilization is a feature of apomictic plants that produce seeds, from which the plants that are clones of the mother plant arise. The phenomenon of apomixis occurs in some sexual plants under specific circumstances. Since the launch of a fertilization-independent mechanism is considered a useful tool for plant breeding, there have been efforts to artificially induce apomixis. We have been able to produce fertilization-independent endosperm in vitro in Arabidopsis over the last few years. This paper demonstrates the methods of improving the quality of the endosperm obtained using plant and mammalian steroid hormones. Additionally, it shows the study on the autonomous endosperm (AE) formation mechanism in vitro. This paper examines the effect of exogenous steroid hormones on unfertilized egg and central cell divisions in culture of unpollinated pistils of Arabidopsis Col-0 wild-type andfie-1 mutant. All media with hormones used (estrone, androsterone, progesterone, and epibrassinolide) stimulated central cell divisions and fertilization-independent endosperm development. The stages of AE development followed the pattern of Arabidopsis thaliana wild type after fertilization. Subsequent stages of AE were observed from 2-nuclear up to cellular with the most advanced occurring on medium with 24-epibrassinolide and progesterone. The significant influence of mammalian sex hormones on speed of AE development and differentiation was noticed. Using restriction analysis, the changes in methylation of FIE gene was established under in vitro condition. The authors of this paper showed that Arabidopsis thaliana has a high potency to fertilization-independent development.
The effect of auxin treatment on the formation of apomictic – endospermless grains in Poa pratensis was investigated in the study. Four polish cultivars: Skiz, Eska 46, Alicja, Ani as well as six breeding lines: POB 13, SK-W-33, SKW-15, SKW-35A, SK-W-35A and SK-W-35B were tested by embryological methods to reveal the reproduction pathway in greenhouse and field conditions. The obtained results showed that four genotypes, i.e. Alicja, Ani, Eska 46 and SK-W-35B are facultative apomictic and the other have formed seeds only on the apomictic way. In facultative apomicts it was found that in many cases both endospermless ovules containing embryo sacs with embryos or embryo sacs with well developed endosperm were formed. In the case of apomictic genotypes only endospermless seeds were observed.
Spatial distribution and genetic variation of a population of Sorbus chamaemespilus (L.) Crantz and putative hybrids between S. chamaemespilus, S. aria and S. aucuparia growing in the nature reserve Skalnä Alpa (central Slovakia) were studied. The analysis of spatial patterns using Ripley's K-function revealed a significant clustering of the adults of both S. chamaemespilus and hybrid taxa at distances up to ~15 m and a strong affinity between both taxonomical groups, indicating similar ecological requirements. Bivariate point-pattern analysis considering cardinal direction showed that juvenile individuals of S. chamaemespilus are clustered around the adults up to the distance of ~2 m, whereas in hybrid taxa with larger and more dense crowns, juveniles are clustered at distances more than ~3 m from the adults. The analysis of genetic variation in a subset of adult shrubs using 4 nuclear microsatellite loci revealed that unlike expected, there was no variation in S. chamaemespilus but several genotypes were found in the group of hybrid taxa. Implications for the reproduction system and conservation of the investigated taxa are discussed.
Kentucky bluegrass is a facultative apomict which is propagated from seeds of variable genetic origins, ploidy levels and nuclear DNA contents. This study analyzes the variability of relative nuclear DNA content among cultivars and natural populations, and examines whether this variability is correlated with morphological traits. Relative nuclear DNA content (an indirect measure of chromosomal variability) was determined in 281 plants from 28 accessions (17 cultivars, 11 populations) using flow cytometry of DAPI-stained nuclei. The same plants were also measured for leaf area and stomatal length. Variation of measured relative DNA content between the studied accessions was very high (5.5-fold). Intra-accession variation was very high in six accessions, even though three of these were cultivars. Relative nuclear DNA content was correlated with stomatal length but not with leaf area. The lack of correlation with leaf area might explain why high intra-accession variability of nuclear DNA content was found in released cultivars that had passed uniformity testing during the registration procedure. We suggest that nuclear DNA content measurement should be made part of the cultivar registration process
The paper reports a study of polyploid progeny of crosses between diploid sexual maternal plants and tetraploid pollen donor plants in the genus Taraxacum sect. Ruderalia. All polyploid progeny plants were triploids; no tetraploids were found. Two types of experiments were done with each plant: crossing of some capitula with diploid pollen donor, and isolation of other capitula. Flow cytometric seed screening, together with analysis of seed set, were used to determine the breeding system of particular hybrids. Of the 29 triploid hybrids studied, 7 plants were apomictic. Seventeen triploid hybrids produced progeny sexually, reduced ovules were fertilized, and seed set was low. Three plants produced (near)tetraploid progeny - BIII hybrids with autonomous endosperm. The remaining 2 triploid hybrids were nonapomicts, but their type could not be distinguished. Compared with the crosses with triploid pollen donors, the crosses of diploid with tetraploid pollen donors produced fewer apomictic progeny and more nonapomictic progeny with reduced, irregular chromosome numbers. However, the total number of developed seeds per capitulum was substantially higher in diploid x tetraploid crosses, and their impact in microevolutionary processes may be considerable. In both types of crosses, diplosporous plants lacking the capacity for parthenogenesis were produced, confirming the breakdown of apomixis into its elements.
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