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Oxidative stress in standardbred horses during official races of 1600 and 2000 meters

100%

Piccione G., Fazio F., Giudice E.

Medycyna Weterynaryjna

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2007

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tom 63

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nr 12

1554-1557

In thoroughbred race horses, as in other species subjected to physical activity, there is a rise in the imbalance between free radical production and antioxidant agents which leads to oxidative stress. This stress may produce damage in several bio-molecules creating metabolic alterations affecting physical performance. The aim of this study was to find possible relationships between physical exercise and oxidative stress in trained horses. In order to achieve this we reported the results obtained while studying the effect of a physical exercise test on two groups of standardbreds. In particular, the study assessed levels of creatine kinase (CK) and aspartate amino transferase (AST) to evaluate possible muscle-cell membrane damage; reactive oxygen species (ROS), thiol antioxidant barrier (SHp) and antioxidant barrier (Oxy-adsorbent) to evaluate oxidative stress. Two groups of healthy standard bred (Ga and Gb) trained for 1600 and 2000 meter races were used for the study. Blood samples from all horses were collected at rest, immediately after racing, and 30 and 6 hours after racing. The ANOVA for repeated measures showed the highly significant effects of training on some of the studied parameters in both groups (Ga and Gb). Our results seem to indicate that in trained standard breeds acute exercise generates free radicals but they are unable to cause possible muscle-cell membrane damage. However, in order to know whether the inhibition of oxidative processes during exercise benefits physical performance, it would be necessary to simultaneously measure parameters relating to work capacity such as blood lactate, heart rate and oxygen consumption, both in basal conditions and at different times after a standardized race.

2

Serum homocysteine and oxidative stress evaluation during exercise in horse

80%

Fazio F., Casella S., Giannetto C., Caola G., Piccione G.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 2

169-174

The aim of the present work was to evaluate the influence of exercise on serum homocysteine and oxidative stress in Thoroughbreds during official races. For our study, ten Thoroughbred mares, 5 years old and clinically healthy, were used. The horses were trained to take part in an official 2100 meter race. Reactive oxygen species (dROMs), antioxidant barrier (Oxy-adsorbent), thiol antioxidant barrier (SHp) and homocysteine (sHcy) were investigated. Blood samples, collected from each horse at rest, immediately after the race, and 30 and 180 min after the end of the race, were collected by jugular venipuncture using vacutainer tubes with no additive for the assessment of dROMs, Oxy-adsorbent and SHp by means of a UV spectrophotometer, while serum total Hey values were determined by high performance liquid chromatography with fluorimetric detection and isocratic elution. Statistical analysis, one way repeated measures analysis of variance (ANOVA), followed by Bonferroni's test, showed statistical differences (P<0.05 was considered statistically significant) for all parameters studied. Moreover, a coefficient of linear correlation (r) was computed for values of dROMs and sHcy for all sampling times but a significant linear regression (r = 0.94) was found only after the race. The variations of sHcy, dROMs, Oxy-adsorbent and SHp during the experimental period considered suggest the important role of oxidative status in the athletic horse. The systematic analysis of oxidative stress and its influence on homocysteine levels contribute to the clinical evaluation and assessment of the athletic performance of the horse.

3

The antioxidative barrier in the organism

70%

Kulikowska-Karpinska E., Moniuszko-Jakoniuk J.

Polish Journal of Environmental Studies

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2004

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tom 13

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nr 1

Cell metabolism in organisms which use oxygen as a source of energy is closely associated with the generation and action of free oxygen radicals and their derivatives. Extra- and intracellular substances that are antioxidative in nature prevent overproduction of radicals and protect against propagation of peroxidative reactions. The list of compounds which can be treated as antioxidants becomes elongated. Many classifications of these compounds are used, of which the most common is the division according to their nature into enzymatic and non-enzymatic, according to their environment or the way they react with FOR. Enzymatic antioxidants include: superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. Non-enzymatic antioxidants are: vitamin E, vitamin C, glutathione, carotenes and retinols, and some transition metals (Zn, Cu and Se). The balance between the actions of these two groups of compounds determines normal functioning of the organism. Impairment of the balance between pro- and antioxidative processes in the organism is called anitoxidative stress and may be induced by intensified reactions involving FOR and by depressed activity/concentration of antioxidants. It seems, however, that irrespective of the cause, oxidative stress is likely to result in many diseases.

Ograniczanie wyników

1 Medycyna Weterynaryjna

1 Polish Journal of Environmental Studies

1 Polish Journal of Veterinary Sciences

2 Fazio F.

2 Piccione G.

1 Caola G.

1 Casella S.

1 Giannetto C.

1 Giudice E.

1 Kulikowska-Karpinska E.

1 Moniuszko-Jakoniuk J.

1 2009

1 2007

1 2004

Oxidative stress in standardbred horses during official races of 1600 and 2000 meters

Serum homocysteine and oxidative stress evaluation during exercise in horse

The antioxidative barrier in the organism