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Application of fast liquid chromatography for antioxidants analysis

100%

Drozdzynska A., Dziedzic K., Kosmider A., Leja K., Czaczyk K., Gorecka D.

Acta Scientiarum Polonorum. Technologia Alimentaria

2012

tom 11

nr 1

Background. An intensive development of the Fast Liquid Chromatography (FLC) has been recently observed. It makes possible to reduce time analysis and improve resolution as well as sensitivity. The aim of this study was to separate the chosen antioxidants optimization using the FLC method. Material and methods. The three various procedures for antioxidants analysis were compared. Mobile phases containing aqueous solution of formie acid, acetic acid, acetonitrile, and methanol were tested. Limit of detection (LOD), limit of quantification (LOQ), linearity and repeatability of each procedures were determined. Results. Developed procedure enabled to separate all analytes and allowed to get low LOD levels and good repeatability. This procedure was used for antioxidants analysis in buckwheat and buckwheat products. Conclusion. Fast Liquid Chromatography allows to reduce time analysis and obtain good validation parameters.

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Micropropagation studies and antioxidant analysis of the endangered plants of Bulgarian yellow gentian (Gentiana lutea L.)

80%

Petrova M. I., Zayova E. G., Dimitrova L. I., Geneva M. P., Miladinova-Georgieva K. D.

Acta Scientiarum Polonorum. Hortorum Cultus

2019

tom 18

nr 3

In order to develop an efficient micropropagation system, it is essential to establish the appropriate concentration of growth regulators for seed germination, shoot formation and rooting. Nodal segments from in vitro obtained seedlings of Gentiana lutea L. were cultured in vitro in Murashige and Skoog’s medium supplemented with BAP, Thidiazuron and Zeatin (0.5, 1.0 and 2.0 mg L−1). A maximum number of shoots with the highest height was recorded at 2.0 mg L−1 BAP. For further optimization of the process, we used nutrient media containing BAP and Zeatin with a combination of low concentration of Indoleacetic acid. MS medium containing 2.0 mg L−1 Zeatin and 0.2 mg L−1 IAA resulted in maximum numbers of shoots 94.3) with shoot height 2.5 cm. The multiple plants were successfully ex vitro acclimatized with 65% survival. The presence of growth regulators (2.0 mg L−1 Zeatin and 0.2 mg L−1 IAA) in the nutrient media resulted in an effective antioxidant activity in G. Lutea determined by the low molecular antioxidant metabolites such as phenols and flavonoids and activities of antioxidant enzymes – catalase, ascorbate peroxidase, guaiacol peroxidase, and superoxide dismutase. The described protocol allows the establishment of numerous micropropaged plants of rare and endangered G. lutea.

Ograniczanie wyników

1 Acta Scientiarum Polonorum. Hortorum Cultus

1 Acta Scientiarum Polonorum. Technologia Alimentaria

1 Czaczyk K.

1 Dimitrova L. I.

1 Drozdzynska A.

1 Dziedzic K.

1 Geneva M. P.

1 Gorecka D.

1 Kosmider A.

1 Leja K.

1 Miladinova-Georgieva K. D.

1 Petrova M. I.

1 Zayova E. G.

1 2019

1 2012

Wyniki wyszukiwania

Application of fast liquid chromatography for antioxidants analysis

Micropropagation studies and antioxidant analysis of the endangered plants of Bulgarian yellow gentian (Gentiana lutea L.)