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The aim of this study was to determine the in vitro resistance of aerobic bacteria isolated from the uterine pathological secretion of 312 dairy cows with clinical metritis and clinical endometritis to antibiotics. Animals with pathological discharges from the vagina observable between the 7th and 50th day after parturition were diagnosed clinically per vaginam and per rectum and then swabs from uteri lumen were aseptically collected. Bacteriological examinations of swabs were performed according to commonly accepted rules. Sensitivity to antibiotics was tested by the disk diffusion method and performed according to CLSI (formerly NCCLS) guidelines in Mueller-Hinton agar. The bacteria isolated were mostly Arcanobacterium pyogenes, Escherichia coli, non E. coli Gram-negative rods, Streptococcus species and Staphylococcus species. Strains of Arc. pyogenes were the most susceptible to amoxicillin/clavulanic acid (97.3%), ceftiofur (98%) and bacitracine (96.7%). E. coli isolates were the most susceptible to norfloxacin (100%), marbofloxacin (100%), rifaximin (97%), gentamycin (96%) and amoxicillin/clavulanic acid (95.5%). Other Gram-negative bacteria were the most sensitive to norfloxacin (100%), neomycin (100%) and cefoperazon (95%). Streptococcus species were the most susceptible to amoxicillin/clavulanic acid (94.6%), ampicillin (92.3%), norfloxacin (92%), cephapirine (88%), cefoperazone (86.5%), rifaximine (85.7%) and penicillin (84.9%). The highest in vitro activity against Staphylococcus spp. was demonstrated by amoxicillin/clavulanic acid (100%), norfloxacin (100%), neomycin (93.6%) and cefoperazone (85.7%). Arc. pyogenes were the most resistant to oxytetracycline and cloxacillin, E. coli to ampicillin and cephapirin, non-E. coli Gram-negative rods to ampicillin and cephapirin, Streptococcus spp. to neomycin and oxytetracycline, and Staphylococcus spp. to ampicillin.
Although integrons by themselves are not mobile, due to their presence in plasmids and transposons, they can be transferred horizontally. For these reasons integrons are a major mechanism for the spread and maintenance of multidrug resistance (MDR). This study describes the distribution of integron gene cassette classes in a collection of uropathogenic Escherichia coli (UPEC) isolated from children with community acquired urinary tract infection in Jahrom, Iran. E. coli strains isolated from urine samples were tested for susceptibility to 14 different antibiotics using the disk diffusion method and for integron classes by RFLP-PCR. Totally 96 strains of E. coli were isolated from urine samples. High prevalence of resistance to ampicillin (80.2%), co-trimoxazole ((76%) and tetracycline (70.8%) was seen among the UPEC isolates. All isolates were 100% sensitive to imipenem. Sixteen strains (16.6%) had the evidence of integron sequences with the prevalence of 6.25% (n = 6) and 10.41% (n = 10) for intI1 and intI2, respectively. No intI3 was detected in the isolates. The presence of integrons was significantly associated with resistance to certain antibiotics including gentamicin and ampicillin. Considering the MDR patterns and the low prevalence of integrons among the E. coli strains under the study, we suggest that the antibiotic resistance cassettes in these strains presumably are mostly carried on the other transposable elements rather than integrons.
Diarrhoea, dysentery and other diseases due to other enteric bacteria have reportedly been found to resist chemotherapeutic treatment in some West African communities with fatal consequences in some cases. This study was carried out to determine multidrug resistance patterns of Enterobacteria isolates from processed ready-to-eat foods. Indigenously processed food samples of different types were collected from two Francophone and two Anglophone countries in the West African sub-region during the wet and dry seasons of a sampling period of two years. Enterobacteria were isolated from the samples using standard techniques. Amplification of chromosomal DNA of the isolates using the Polymerase Chain Reaction was carried out. The results obtained were subjected to statistical analyses. All isolates showed resistance to cefuroxime (90.7%), nitrofurantoin (90.6%), augmentin (86.1%) and ampicillin (51.2%) while all were sensitive to gentamycin and ciprofloxacin. There was amplification indicating the presence of invA gene at a position of 240 bp. There was no amplification at all for the spvC gene in any of the isolates tested. Multidrug resistant enteric bacteria in these foods containing the invA gene could lead to infections with uncontrolled antibiotic use. The presence of enteric bacteria in the foods analyzed which provide undeniable evidence of the poor microbiological quality of these foods could form the basis of a useful databank in formulation of food-borne disease control and prevention strategies.
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