The cytotoxic activity of petroleum ether extract of the leaves of Cassia roxburghii Linn. against HCT-116 and MCF-7 cell lines resulted with IC50=34.9 and 38.04 μg/ml, respectively, while against HepG-2 showed no activity. A bioassay guided-fractionation approach was conducted to isolate and identify the active cytotoxic principles. Further chromatographic separation and purification of the petroleum ether extract resulted in the isolation of two anthraquinones identified as aloe-emodin acetate and aloe-emodin, along with stigmasterol, β-sitosterol and palmitic acid. The structure elucidation of isolated compounds was performend using 1D, 2D-NMR and HR-MS. Furthermore, the cytotoxicity of aloe-emodin acetate and aloe-emodin were evaluated and resulted with IC50=153.30 and 70.02 μg/ml against HCT-116 and with 93.20 and 53.20 μg/ml against MCF-7, respectively, while against HepG-2 showed no activity. Moreover, the antiviral activity of the two isolated anthraquinones was tested against influenza virus-A, and resulted with IC50=10.23 as well as 2.00 and with CC50=1.32 and 0.47 μg/ml, respectively.
Effects of four auxins (2,4-dichlorophenoxyacetic acid, a-naphthaleneacetic acid, ß-indole- acetic acid or ß-indoiebutyric acid) at the concentrations of 0.1, 1 or 10 mg/I on the cell growth and anthraquinone production in Rheum palmatum L. callus cultures were studied. The highest growth was obtained by lmg/l 2,4-D and 10 mg/l NAA (growth index 9.29 and 9.60, respectively), the best anthraquinone production was achieved by 1 mg/1 NAA, 10 mg/1 NAA, 1 mg/l IAA and 0.1 mg/1 IBA (5.24-5.51 mg/g diy weight).
Four separate analytical procedures were applied to determine polycyclic aromatic hydrocarbons (PAH's) and their oxygen and nitrogen derivatives including carbazoles, nitrocompounds and aza compounds. The basic method applied in all four procedures was chromatography, utilized in different variations aimed at obtaining PAH concentrates and their derivatives, before identification analysis. Capillary gas chromatography and the GC-MS system were applied in the identification of individual compounds and in quantity determinations. In the airborne particulate matter in Upper Silesia — the Polish region considered to be the most ecologically degraded — the following compounds have been determined among many others: several PAH's; oxygen derivatives such as 9-fluorenone, anthraquinone, benzanthrone, 1-nitropyrene and/or 2-nitrofluoranthene; carbazole; quinoline; and benzoquinoline. Except for PAH, the enumerated compounds have so far not been determined in environmental samples all over the country.
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