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There is a growing interest in dietary therapeutic strategies to combat oxidative stress-induced damage to the Central Nervous System (CNS), which is associated with a number of pathophysiological processes, including Alzheimer’s and Parkinson’s diseases and cerebrovascular diseases. Identifying the mechanisms associated with phenolic neuroprotection has been delayed by the lack of information concerning the ability of these compounds to enter the CNS. The aim of this study was to evaluate the transmembrane transport of flavonoids across RBE-4 cells (an immortalized cell line of rat cerebral capillary endothelial cells) and the effect of ethanol on this transport. The detection and quantification of all of the phenolic compounds in the studied samples (basolateral media) was performed using a HPLC-DAD (Diode Array Detector). All of the tested flavonoids (catechin, quercetin and cyanidin-3-glucoside) passed across the RBE-4 cells in a time-dependent manner. This transport was not influenced by the presence of 0.1% ethanol. In conclusion, the tested flavonoids were capable of crossing this blood-brain barrier model.
The aim of the research was to analyse the weaknesses of the pH differential method for strawberry anthocyanin determination. The work is based on practical experiments with 12 strawberry cultivars and on analysis of published papers. We used following molar absorption coefficients ( ƹ values): 26900 and 29600 M⁻¹ cm⁻¹ for cyanidin 3 glycoside (C3g) and 15600, 22400, 27300 and 36000 M⁻¹ cm⁻¹ for pelargonidin 3 glycoside (P3g). In order to show how the calculated value of total anthocyanins depends on the predominant anthocyanin used for the calculations, we compared the results of spectroscopic and chromatographic analysis. Present research demonstrated that different ƹ values may influence the results of total anthocyanins even more than cultivar properties. The most frequently used ƹ values 26900 M⁻¹ cm⁻¹ and 29600 M⁻¹ cm⁻¹ gave underestimated values. C3g was present in minor amounts in all cultivars. Conclusively, P3g with the ƹ = 15600 M⁻¹ cm⁻¹ should be used for ensuring most precise estimation of total anthocyanin content in strawberries.
The total polyphenols content in investigated plums, as well as anthocyanins and flavanols, was rather low (160-300 mg/100 g, 1833 mg/100 g and 914 mg/100 g of fruits, respectively). (+) Catechin occurring in quantities from 1.3 to 3.9 mg/100 g was found to be a dominant compound representing biologically active monomers and dimers of flavanols. The highest quantities of hydroxycinnamic acids, especially neochlorogenic one, (46-85 mg/100 g) are found in all plum varieties. The main anthocyanin pigments of plums are cyanidin-3-glucoside and cyanidine-3- rutinoside; they make up 42 to 62% of the total anthocyanins. All investigated plum varieties show high polyphenoloxidase activity but there are substantial differences between particular varieties ranging from 3200 to 17200 U/g.
A qualitative and quantitative analysis of anthocyanins in juices of three varieties of strawberry (Senga, Ducat, Marmolada), raspberry (Beskid, Canby, Malling Seedling), black currant (Ben Lomond, Titania, Ojebyn) and red currant (Rondom, Jonker, Holenderska) picked in three following years: 1998, 1999 and 2000, was presented in this paper. An HPLC technique was applied using a Gilson chromatograph and a DAD detector. Prior to the chromatographic analysis, anthocyanins were purified on a mini-column Sep-Pak C18 Waters. It was indicated that within species the juices examined differed in the quantitative and qualitative composition of anthocyanins. Pelargonidin-3-glucoside and cyanidin-3-xylorutinoside were the main anthocyanins in strawberry and red currant juices, respectively, independently of variety. Those anthocyanins were not detected in raspberry and black currant juices, in which cyanidin-3-sophoroside as well as delphinidin-3-rutinoside and cyanidin-3-rutinoside were the main anthocyanins, respectively. Differences of anthocyanin composition of juices obtained from different berry fruits create the possibility of detecting the adulterations of expensive raspberry and black currant juices with cheap strawberry and red currant juices on the basis of anthocyanin analysis.
In Crassula multicava Lam. anthocyanins are formed naturally mostly in the stem near nodes and only traces in other parts of internodes. Methyl jasmonate (JA-Me) applied in lanolin paste at concentrations of 0.05, 0.1, 0.5 and 1.0% on the middle part of internodes greatly stimulated anthocyanins accumulation in the internodes and in the nodes of Crassula multicava. The stimulatory effect was higher in younger tissues of the Crassula multicava stem than in older ones, and depends on the used concentration of JA-Me. The possible role of jasmonates on anthocyanins formation in Crassula multicava is discussed.
Sulphide-2-chloroethyl-3-chloropropyl is an alkylating agent. It posssesses mutagenic and carcinogenic properties, participates in oxidative processes and can induce lipid peroxidation.The aim of our investigation was to define antioxidative activity of natural anthocyanins after single experimental intoxication with sulphide-2-chloroethyl-3-chloropropyl in mice. Catalase activity in hemolysate, thiobarbituric acid reacting substances (TBARS) concentration in hemolysate and selected organs were determined. The study confirms increased lipid peroxidation as a result of sulphide-2-chloroethyl-3-chloropropyl intoxication, but natural anthocyanines derived from Aronia Melanocarpa also seem to play a protective role as an antioxidative agent.
The fruit of black chokeberry (Aronia melanocarpa (Michx.) Elliott) is valuable raw material for food and pharmaceutical industries due to the content of anthocyanins, but also other components. The aim of this study was to estimate the content of dry matter, reducing sugars, vitamin C, anthocyanins and the antioxidant potential in black chokeberry fruits depending on the age of plants and the time of fruit harvest. Material for the study was collected in 2011 and 2012 from 6–7, 11–12 and 16–17-year-old plants. Fruits for analyses were harvested on 10–12 August, 29 August – 1 September, 11–13 September, 26–27 September, 11–14 October, 25–27 October. Fruits from the oldest plants contained 16–18% less anthocyanins and 8% less vitamin C, but 2.7 percentage points more dry matter than the fruits from younger plants. On the 29 August–1 September fruits achieved the maximum content of reducing sugars, vitamin C and anthocyanins, 13.7, 8.2 and 715.5 mg 100 g-1, respectively. The content of reducing sugars and anthocyanins stayed unchanged until the end of October, and the content of vitamin C decreased considerably. Antioxidative potential (DPPH) ranged from 77.0 to 85.2% inhibition and it did not depend on the age of harvest time of fruits. The most valuable chokeberry fruits were obtained from plants up to 12 years of age, harvested between 29 August and 13 September.
Effects of various jasmonates (methyl jasmonate, jasmonic acid, cis-jasmone) on anthocyanins and procyanidins content of, as well as on growth of common buckwheat (Fagopyrum esculentum Moench) seedlings were studied. The studied jasmonates were applied as solutions or vapors on four days seedlings, and the seedlings were grown during the next four days in day/night conditions (16/8 h). Afterwards anthocyanins and proanthocyanidins content, as well as elongation of primary roots and hypocotyls were measured. When applied as solutions cis-jasmone (JAS) stimulated the anthocyanins accumulation, but when used as vapors had tendency to decrease its accumulation in buckwheat hypocotyls. Jasmonic acid (JA) solutions slightly stimulated or had no effect on biosynthesis of anthocyanins in buckwheat hypocotyls, but used as vapors caused a decline of anthocyanins in buckwheat hypocotyls. Methyl jasmonate (MJ) clearly inhibited biosynthesis of anthocyanins in hypocotyls of buckwheat seedlings. The studied jasmonates had no influence on anthocyanins level in cotyledons of buckwheat seedlings, except cis-jasmone, which at the lowest solution concentration slightly enhanced biosynthesis of the pigments. Treatment of buckwheat seedlings with solutions of all jasmonates (10-8 M, 10-6 M and 10-4 M) had no influence on the growth of buckwheat hypocotyls. Contrary to that observation vapors of the growth regulators in concentrations 10-4 M, had a strong inhibitory effect on the growth of hypocotyls of buckwheat seedlings. Solutions of JA and MJ, as well as vapors of JA, MJ and JAS strongly inhibited the primary root growth of buckwheat seedlings, while JAS applied as solution had no such influence. MJ and JA caused much higher stimulation of proanthocyanidin biosynthesis in buckwheat hypocotyls than JAS.
In precise field trials on three locations in the Czech Republic the impact of yellow, red and purple-fleshed potato cultivars in the years 2006 and 2007 was investigated as well as the effect of location conditions on antioxidant activity (AA) of tubers (expressed by ABTS assay in mg ascorbic acid 100 g⁻¹1 FM). The highest AA was determined in the darkly purple-fleshed cv. Violette (95.4 mg ascorbic acid·100 g⁻¹ FM). In the purple and red-fleshed cultivars, AA was on the average 2.9 times higher as compared to yellow-fleshed cultivars. Significant AA differences between purple and red-fleshed cultivars were found; the cultivars of the highest total anthocyanin content (TAC ranged from 18.6 to 64.1 mg·100 g⁻¹ FM) were reaching the highest AA values. The obtained results did not prove the impact of location on AA of tubers.
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