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The studies were conducted in the years 1998–2000 on soybean, cv. Polan. The purpose of the studies was to conduct microbiological and mycological analyses of the leaves and pods of soybean. Bacteria and fungi were obtained much more often from the diseased soybean parts as compared to the healthy ones. 778 fungi isolates were obtained as a result of the mycological analysis of the leaves and pods of soybean. Fusarium spp. and Phomopsis sojae were most frequently isolated within pathogenic fungi. Their proportion was 17.5% and 7.8% of all the fungi, respectively. On the other hand, the isolated saprophytic fungi included for example the species from the genera of Acremonium, Cladosporium, Gliocladium, Penicillium and Trichoderma as well as Aureobasidium pullulans and Epicoccum purpurascens. Besides, about a four times lower number of antagonistic microorganisms (Bacillus spp., Pseudomonas spp., Gliocladium spp., Penicillium spp., Trichoderma spp.) was isolated from the infected soybean parts in comparison to the number of microorganisms isolated from the healthy parts.
W badaniach określono wpływ ekstraktu z grejpfruta i fungicydu Miedzian 50 WP na zdrowotność grochu oraz na kształtowanie się populacji mikroorganizmów w ryzosferze tej rośliny. Liczba roślin wyrosłych z nasion zaprawianych Grevitem 200 SL bywa zbliżona, a nawet przewyższa, ale nie statystycznie istotnie, liczbę roślin uzyskanych po zastosowaniu fungicydu Miedzian 50 WP. Wartość indeksu porażenia roślin była najmniejsza po wykorzystaniu Grevitu 200 SL, ale statystycznie nie różniła się od tej dla Miedzianu 50 WP. Z porażonych roślin izolowano m. in. Alternaria alternata, F. culmorum, F. oxysporum, F. solani, Phoma eupyrena, Pythium irregulare, Rhizoctonia solani i Sclerotinia sclerotiorum. Z ryzosfery izolowano m. in. Alternaria alternata, Fusarium spp., Gliocladium spp., Mucor spp., Penicillium spp., R. solani, S. sclerotiorum i Trichoderma spp. Liczebność bakterii ryzosferowych w kombinacjach z Grevitem 200 SL i Miedzianem 50 WP byáa istotnie większa, aniżeli w kombinacji kontrolnej. Odwrotna zależność wystąpiła w przypadku liczebność grzybów, ale statystycznie mniej grzybów wystąpiło w kombinacji z Grevitem 200 SL. Mikroorganizmy antagonistyczne dominowały w ryzosferze roślin wyrosłych z nasion zaprawianych Grevitem 200 SL.
The die-back of neem caused by Phomopsis azadirachtae is a devastating disease in India reducing the life span and seed production of neem. Six isolates of antagonistic bacteria and fungi, Bacillus cereus (MTCC 430), B. subtilis (MTCC 619), Pseudomonas aeruginosa (MTCC 2581), P. oleovorans (MTCC 617), Trichoderma harzianum (MTCC 792) and T. viride (MTCC 800) were evaluated against P. azadirachtae under in vitro conditions. Culture filtrates of all these microorganisms were extracted using ethyl acetate, and the obtained fractions were tested for their antifungal activity against P. azadirachtae at different concentrations. Ethyl acetate extracts of B. subtilis and P. aeruginosa were highly effective and completely inhibited the growth of P. azadirachtae at 25 ppm concentration. Both these isolates may be considered as factors for the biological control of die-back of neem.
The purpose of the studies carried out in the years 1996-1998 was to establish the composition of bacteria and fungi communities in the potato rhizosphere and non-rhizosphere soil. Besides, in the examined samples the studies established the proportion of bacteria and fungi antagonistic towards soilborne pathogens. The microbiological analysis of 1 g of dry weight of soil coming from the rhizosphere of potato revealed from 3.96 x 10⁶ to 7.26 x 10⁶ bacteria colonies and from 51.38 x 10³ to 69.96 x 10³ fungi colonies. In the case of nonrhizosphere soil of 1 g of dry weight of soil revealed from 3.50 x 10⁶ to 4.75 x 10⁶ bacteria colonies and from 16.16 x 10³ to 34.10 x 10³ fungi colonies. Moreover, potato cultivation had a positive effect on the increase of numbers of antagonistic bacteria (Bacillus spp. and Pseudomonas spp.) and fungi (Gliocladium spp., Penicillium spp., Trichoderma spp.). A larger number of the communities of bacteria and fungi, including antagonistic ones, in the root area of potato, indicates considerable biological activity, which contributes to a better phytosanitary condition of the soil.
The study determined the antagonistic effect of microorganisms of the soil environment of salsify towards the following pathogenic fungi: A. alternata, F. culmorum, F. oxysporum, R. solani and S. sclerotiorum. The field experiment considered mulching the soil with such cover crops as oats, phacelia and common vetch. Laboratory tests pointed to oats as the plant which has the best influence on the quantity of antagonistic Bacillus spp., Pseudomonas spp., Gliocladium spp., Penicillium spp. and Trichoderma spp. These microorganisms reduced the growth of A. alternata and F. culmorum in the most effective way. Antagonistic fungi inhibited the growth of F. oxysporum, R. solani and S. sclerotiorum in a greater degree than antagonistic bacteria. Mulching the soil with cover crops can reduce the growth and development of soil-borne fungi pathogenic to salsify.
The purpose of the studies carried out in the years 1996-1998 was to establish the composition of bacteria and fungi communities in the soil of spring wheat and winter wheat. Besides, the studies provided the information on quantitative and qualitative composition of amino acids as well as the amount of water soluble sugars in roots exudates of these cereals. The microbiological analysis of 1 g of dry weight of soil coming from the rhizosphere of spring wheat revealed the mean number of 4.94 x 10⁶ bacteria colonies and 45.08 x 10³ fungi colonies. In the case of winter wheat, in 1 g of dry weight of the rhizosphere soil 5.07 x 10⁶ bacteria colonies and 28.59 x 10³ fungi colonies (mean of three year studies) were found . Besides, winter wheat, through the proper composition of root exudates, created positive nutritional conditions for the growth of microorganisms showing antagonistic effect towards pathogenic fungi. The rhizosphere soil of winter wheat contained 1.5 times more antagonistic bacteria and more than twice antagonistic fungi as compared to spring wheat.
The purpose of the studies conducted in the years 1996-1998 was to determine the quantitative composition of bacteria and fungi populations in the rhizosphere of spring wheat, winter wheat, potato and soybean, and in non-rhizosphere soil. Besides, the effect of root exudates of these plants on the formation of antagonistic microorganisms is presented. A microbiological analysis found out that 1 g of rhizosphere soil dry weight of the examined plants and non-rhizosphere soil contained from 4.24 x 10⁶ to 5.97 x 10⁶ bacteria colonies on average. The lowest number of bacteria was found in non-rhizosphere soil (4.24 x 10⁶ on average), and the highest in rhizosphere of potato (5.97 x 10⁶ on average). The fewest fungi colonies (28.59 x 10³ on average) were isolated from 1 g of dry weight of winter wheat rhizosphere, and the most (93.41 x 10³) from soybean rhizosphere. Antagonistic bacteria of genera Bacillus and Pseudomonas, and fungi of Gliocladium, Penicillium and Trichoderma genera dominated in winter wheat rhizosphere. Soybean roots exuded the greatest number of aminoacids (1.088 mg/ml of the solution), while spring wheat roots exuded the smallest amount (0.148 mg/ml of the solution). The percentage of aromatic and alkaline aminoacids was the lowest in potato root exudates, whilc the highest was found out in the exudates of winter wheat.
Grain fusariosis are caused by phytopathogenic fungi from genus Fusarium, which due to their high pathogenic properties and toxicity contribute to enormous farming loses. In crop farming they lower grain harvests and worsen its quality, especially the ability to germinate and baking parameters. Most of the wheat diseases are caused by fungi from genera F. culmorum, F. graminearum, F. nivale, F. avenaceum, F. sporotrichioides, F. poae, F. oxysporum and F.verticillioides. Chemical fungicides are often used to control fusariosis, however, despite their effectiveness and easy application, they have not gained good recognition. Phytopathogenic fungi become resistant to fungicides, they reduce beneficial organisms and endanger the health of a consumer. It has led to a search of alternative methods of controling grain fusariosis, with the application of effective biodegradable fungicides and products containing active substances of natural origin or antagonistic microorganisms.
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