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In this study, the biocontrol abilities of water-soluble and volatile metabolites of three different isolates of Trichoderma (T. asperellum,T. harzianum and Trichoderma spp.) against soil borne plant pathogen Rhizoctonia solani were investigated both in vitro and in vivo. The results showed for the first time that mycelial growth inhibition of the pathogen was 74.4–67.8% with water-soluble metabolites as compared to 15.3–10.6% with volatile metabolites in vitro. In vivo antagonistic activity of Trichoderma isolates against R. solani was evaluated on bean plants under laboratory and greenhouse conditions. We observed that T. asperellum was more effective and consistent, lowering disease incidence up to 19.3% in laboratory and 30.5% in green house conditions. These results showed that three isolates of Trichoderma could be used as effective biocontrol agents against R. solani.
The morphological characterization was carried out for 5 isolates of Trichoderma harzianum and 7 isolates of Trichoderma viride and tested for their biocontrol efficacy. The isolates belonging to T.harzianum were analogous in colony colour, culture smell, mycelial colour, conidiation, conidial shape, conidial wall and conidial colour. Correspondingly the isolates of T.viride showed certain similarity in colony colour, colony edge, culture smell, conidiophore branching, conidial wall, conidial colour and chlamydospores. Inter specific differences through cluster analysis based on morphological characters grouped the twelve isolates into three major clusters where all the isolates of T.harzianum formed a single cluster while the isolates of T.viride were bifurcated into two groups. The clustering was substantiated by similarity index which showed maximum similarity among T.harzianum isolates with only less than 20% variation among themselves. Similarly the clusters having isolates of T.viride also had less variation within them. The biocontrol efficacy of these twelve isolates of Trichoderma was experimented by dual culture test under laboratory condition and there existed some relation between the biocontrol efficacy of these isolates and morphology.
It is possible to obtain malt beverage, which includes high number of viable lactic acid bacteria and has a good sensor quality for eight weeks of storage at the temperature of 22°C. L. plantarum ATCC 4080 strain after four weeks of storage did not reveal antagonistic activity against spoilage and pathogenic bacteria. This strain after two, six and eight weeks of storage had antagonistic properties. The tested strain after two and four weeks of storage did not survive during incubation at pH 2.5 and next in malt beverage with 3 mmol/dm3 deoxycholate sodium, while survived in these conditions after six and eight weeks. In case of incubation at pH 2.5 and next in aqueous solution of deoxycholate sodium tested strain after four and six weeks of storage had survival ability. The survival ability in these conditions of the tested strain after two and eight weeks of storage were not investigated
The study determined the antagonistic effect of microorganisms of the soil environment of salsify towards the following pathogenic fungi: A. alternata, F. culmorum, F. oxysporum, R. solani and S. sclerotiorum. The field experiment considered mulching the soil with such cover crops as oats, phacelia and common vetch. Laboratory tests pointed to oats as the plant which has the best influence on the quantity of antagonistic Bacillus spp., Pseudomonas spp., Gliocladium spp., Penicillium spp. and Trichoderma spp. These microorganisms reduced the growth of A. alternata and F. culmorum in the most effective way. Antagonistic fungi inhibited the growth of F. oxysporum, R. solani and S. sclerotiorum in a greater degree than antagonistic bacteria. Mulching the soil with cover crops can reduce the growth and development of soil-borne fungi pathogenic to salsify.
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