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The effect of three foliar-applied Ca-containing preparations on the anatomical features of leaves of sweet pepper (Capsicum annuum L.) was studied. The following preparations were used: Ca(NO3)2, Librel Ca and Wapnowit, applied at the respective concentrations of 0.5%, 1%, 1%, which corresponded to a content of 2000 mg Ca 􀂉 dm-3. Light and scanning electron microscopy were used in the study. It was demonstrated that in amphistomatic bifacial pepper leaves numerous specialised cells occurred which accumulated calcium oxalate crystals in the form of crystalline sand. Anisocytic stomata were found with a much greater density in the abaxial epidermis. They were characterized by very well-developed outer cuticular ledges. It was found that in the leaves of the plants sprayed with the nutrient supplements with increased Ca content there was a much smaller number of epidermal cells per 1 mm2 than in the control plants. These cells were distinguished by an increased size. In the case of the application of the nutrient supplements Librel Ca and Wapnowit, the number of stomata also decreased. However, the application of the calcium supplements resulted in an increase in the value of the stomatal index compared to the control, which is attributable to a significant reduction in the number of epidermal cells not belonging to the stomata. The plants additionally supplied with Ca were marked by a larger number of colenchyma layers and an increased volume of leaf parenchyma cells. In the case of pepper leaves, the thin cuticle and the outer cell wall are not a major barrier to the Ca-containing preparations applied for spray treatment. Nevertheless, the decrease in the number of stomata may restrict the possibility of Ca uptake by this way, which compensates the increase in surface area of particular epidermal cells that will be the main way of Ca penetration into the internal leaf tissues.
Cycads are an ancient lineage of plants that originated in the Permian, which are vital to the interpretation of plant ecology. The evidence in the fossil records indicates that the morphological and anatomical features of cycads are remarkably similar to the extant taxa, which has been instrumental in our understanding the connections between the early origins of seed plants and their present-day counterparts. The cycad ecosystem is an important vegetation type throughout geological time. Research on the ecological function of the cycad plays a significant role in the study of evolutionary ecology. In this study, we investigated the biomass, productivity and total carbon storage (total of vegetation, litter, and soil carbon) of cycad (Cycas panzhihuaensis L. Zhou et S.Y. Yang) ecosystems in the National C. panzhihuaensis Reserve of China (latitude 26°37′, longitude 101°35′, at 1635 m altitude) by applying the site-standard tree sampling harvest.Cycads are considered to be rare and endangered species, and are in the list of key protected wild plants in the world. The National C. panzhihuaensis Reserve is in Southwestern China, which area approximately 1358 ha, growing approximately 20 000 C. panzhihuaensis individuals. 20 sample plots, each 5 × 5 m were established in the spring of 2006. The mean height of cycads within the stand was 0.44 m and the mean basal diameter was 23.2 cm. The biomass and productivity data for other communities was compiled from references published over the past 20 years throughout China. The biomass and productivity of cycad ecosystems (8.102 ± 6.880 t C ha⁻¹ and 1.183 ± 0.975 t C ha⁻¹ yr⁻¹, respectively) are smaller than tree fern (Alsophila spinulosa (Wall. ex Hook.) R. M. Tryon) or gymnosperm (Pinaceae, Cupressaceae or Taxodiaceae for representative) ecosystems. The community biomass of Pinaceae-, Cupressaceae- or Taxodiaceae-dominated ecosystems are 6.8, 5.4, and 5.3 times larger than the cycad ecosystem, respectively. The productivity of each is 2.3, 2.8 and 3.8 times larger than the cycad ecosystem. Cycad is an ancient dioecious plant. However, the results show that the differences between the biomass of male and female cycads, as well as the productivity, are not significant.
To investigate whether the fungicide Azoxystrobin improves the potential to maintain physio-biochemical functions, tomato plants were grown under either well-watered and deficit irrigation conditions. Drought-stressed tomato plants showed significant reductions in cell membrane stability (CMS), relative water content (RWC), relative water loss (RWL) and chlorophylls, growth attributes and leaflet and main stem anatomical features, while exhibited increases in contents of proline and total phenols, activities of catalase (CAT), peroxidase (POD) and polyphenol oxidase (PPO), fresh (FW) and dry (DW) weights of roots, and leaflet spongy tissue thickness compared to well-watered control plants. Under full irrigation, Azoxystrobin treatment significantly increased RWC and chlorophyll content, POD and PPO activities, root DW, number of fruits per plant and many features of leaflet and main stem, while significantly decreased CMS and RWL, root, shoot and plant lengths, shoot and plant FW, and stem xylem tissue thickness compared to the control plants sprayed with water. However, Azoxystrobin treatment ameliorated drought stress in tomato plants and significantly increased CMS and free proline content, activities of CAT, POD and PPO, and contents of free and total phenols, and root DW and number of fruits per plant, in addition to spongy tissue thickness of leaflet, but not affected chlorophylls and carotenoids contents, root FW, plant DW and most of anatomical features compared to the stressed plants without Azoxystrobin treatment. These results support that Azoxystrobin foliar application may have a positive effect on well-watered and drought-stressed tomato plants.
Przedstawiono wyniki badań dotyczących wpływu zróżnicowanych stężeń glina w pożywce na morfologię i zmiany anatomiczne tkanki korzeniowej pszenicy Henika i pszenżyta Debo. Na podstawie zdjęć mikroskopowych, stosunku masy korzeniowej do masy części nadziemnych oraz zdolności roślin do regulowania odczynu pożywki, stwierdzono, że badane pszenżyto charakteryzowało się większą odpornością na stres. Słowa kluczowe: kwasowość gleby, stres glinowy, anatomia korzenia, morfologia korzenia. Wykonane badania świadczą o silnej reakcji badanych roślin na stres glinowy. Widoczne są indywidualne różnice w zachowaniu obydwu badanych gatunków roślin, związane prawdopodobnie z różnicami ich tolerancyjności na zastosowane warunki stresowe.
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