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Pinus eldarica has been introduced to Iran from more than 800 years ago. Some individuals of this pine have altered both in shape and growth rate in northeastern of Iran, and generated two distinct morphotypes which are called Conical-shaped and Ball-shaped pines. This study was conducted to discriminate these morphotypes using macromorphological and anatomical characteristics. Results of macromorphological analysis showed significant differences both with univariate and multivariate analysis and consequently two new morphotypes were clearly separated from Mondell pine individuals. Furthermore, anatomical differences observed in Conical-shaped pine in comparison with two other pines, from some valuable taxonomical point of view traits such as cross-section form of needle, number of stomata per area, number and position of resin ducts etc. Furthermore, the existent difference in traits like cuticle thickness, stomata density, needle perimeter and length, state increasing the adaptation potential to aridity in Conical-shaped pine in comparison with two others. The differences of two new morphotypes demonstrate that they are new variants of Mondell pine and it is need to be used molecular markers and phylogenic studies for specifying the cause of these morphological and anatomical differences.
The objective of this study was to examine the influence of water temperature (10,17 and 21ºC) on the sex differentiation process in peled. A high temperature, even a sub-lethal one, was not observed to have an influence on the sex ratio in this species; from day 81 after hatching until the end of the experiment the sex ratio in all three experimental groups was 1:1. It was noted, however, that thermal conditions determine the rate of sex differentiation and, in particular, of cytological differentiation. The first female-line cells, oogonia, were observed on day 102 after hatching in the peled kept in water at a temperature of 10ºC. Cytological differentiation was observed in the fish that were reared in higher temperatures as soon as day 81 after hatching. The first symptoms of anatomical differentiation were noted at this time in fish from all experimental groups. Two types of gonads were observed in histological cross-sections; one had a thicker anterior part with the generative and somatic parts located separately (ovaries), and the other had small, spindle-like gonads (future testes). No cytological differentiation of the male sex cells was observed during the experiment.
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