Wyniki wyszukiwania

1

Cross species amplification of coat colour genes in nutrias (Myocastor coypus Mol)

100%

Migdal L., Zabek T., Migdal A., Lapinski S., Bieniek J., Bugno-Poniewierska M.

Folia Biologica

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2016

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tom 64

|

nr 2

2

Amplification of the coat protein gene of apple stem pitting virus [ASPV]

100%

Komorowska B., Malinowski T., Zawadzka B.

Phytopathologia Polonica

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1999

|

tom 17

3

Abnormal FHIT gene transcript and c-myc and c-erbB2 amplification in breast cancer

100%

Kowara R., Golebiowski F., Chrzan P., Skokowski J., Karmolinski A., Pawelczyk T.

Acta Biochimica Polonica

|

2002

|

tom 49

|

nr 2

Searching for ways to improve the characterization of breast cancer we examined the relationship between the status of the FHIT gene transcript and amplification of c-myc and the c-erbB2 oncogene. Abnormal FHIT transcript was detected in 32 of 79 cancers examined. The presence of Fhit protein estimated by Western blots was evident only in cancers exhibiting a normal-sized FHIT transcript. This indicates that abnormal FHIT transcripts observed in our study did not encode any Fhit protein or the amount of such protein was very low. There was no association between the presence of aberrant FHIT gene transcript with age, tumor size, estrogen and progesterone receptor status, local metastases and histological grading. However, the abnormalities in FHIT gene transcripts were observed with different frequency depending on the histopathological type of the tumor. The aberrant FHIT transcript was detected in 60% of lobular cancers and only in 28% of ductal cancers. Analyzing the occurrence of c-myc and c-erbB2 amplification and the presence of aberrant FHIT gene transcripts we found that the aberrant FHIT transcript more frequently occurred in tissues with c-myc amplification. There was a significant (P < 0.05) correlation between the occurrence of the aberrant FHIT gene transcript with accompanying c-myc amplification and positive lymph node status. However, in order to evaluate the predictive value of these findings in breast cancer, an extended clinical follow up will be necessary.

4

Characterisation of cross-amplified microsatellite markers in the red-breasted flycatcher Ficedula parva

84%

Mitrus J., Mitrus C., Rutkowski R., Sikora M., Suchecka E.

Annales Zoologici

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2013

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tom 63

|

nr 4

We tested 24 microsatellite markers for the red-breasted flycatcher, Ficedula parva, using the primers for the PCR reaction described for other species from genus Ficedula. The amplification efficiency, specificity of the products, and polymorphism of cross-amplified microsatellites were determined based on the genotypes of 65 adult males from a natural population breeding in Białowieża Forest (Eastern Poland). Based on obtained results, we identified 9 highly polymorphic microsatellites, consistently amplifying in majority of individuals. Among those markers between 4 and 26 alleles per locus (mean 15.4) were found and the observed heterozygosity ranged from 0.393 to 0.939. The majority of loci (except for Fhy350 and Fhy458) were in Hardy-Weinberg equilibrium. Accordingly, the values of F IS did not significantly differ from zero 0, except for the locus Fhy350. We suggest that this locus could be loaded with high frequency of null alleles. The polymorphic information content (PIC) for the selected loci set was high and in all cases exceeded 0.82. In addition, we calculated, for each locus, the probability of excluding an improper parent. In majority of loci this parameter distinctly exceeded 0.5. These results demonstrate that tested microsatellite markers can be used to estimate the genetic variability within- and between populations and to establish paternity and parenthood in red-breasted flycatcher populations.

5

The increase in mitochondrial DNA copy number in the tissues of gamma-irradiated mice

84%

Malakhova L., Bezlepkin V. G., Antipova V., Ushakova T., Fomenko L., Sirota N., Gaziev A. I.

Cellular and Molecular Biology Letters

|

2005

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tom 10

|

nr 4

6

Use of cytochrome b polymorphism for species identification of biological material derived from cattle, sheep, goats, roe deer and red deer

67%

Natonek-Wisniewska M., Slota E., Kalisz B.

Folia Biologica

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2010

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tom 58

|

nr 1-2

47-50

7

PCR amplification and DNA sequence analysis of parasitic intestinal protozoa in specimens stained with Chlorazol Black E

67%

Morimoto N., Korenaga M., Nishida Y., Takeuchi H., Kumon Y., Sugiura T.

Acta Parasitologica

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2013

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tom 58

|

nr 2

Chlorazol Black E (CBE) stain has been used for the detection and identification of intestinal parasitic protozoa. In recent years, genotyping of protozoa has been performed to examine pathogenicity and for epidemiologic analysis. In this study, protozoan DNA was amplified from preserved human fecal specimens stained with CBE that were positive for Giardia intestinalis (syn. G. lamblia and G. duodenalis), Chilomastix mesnili, Pentatrichomonas hominis, and Entamoeba histolytica. DNA was amplified from 11 of the 12 (91.6%) samples examined. DNA from CBE-stained smears of G. intestinalis, E. histolytica, and P. hominis was amplified, whereas any amplification product could not be obtained from one of three smears of C. mesnili. Storage term and protozoan number had no association with results of PCR amplification. In genotyping of G. intestinalis, four out of six (66.7%) samples were of genotype AI, while the remaining two (33.3%) samples were of genotype B. The amplified DNA sequences showed high similarity (>99%) with that of G. intestinalis in the GenBank database. These results suggest that DNA remains stable in CBE-stained smears for long term. The present study demonstrates that nuclear extracts from specimens stained with CBE can be amplified by PCR and suggests that specimens stored for extended periods could be applied to genetic and prospective epidemiologic analyses.

8

Evaluation of a novel method based on amplification of DNA fragments surrounding rare restriction sites [ADSRRS-fingerprinting] for typing bacterial strains

67%

Krawczyk B., Lewandowski K., Kur J.

Advances in Agricultural Sciences

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2002

|

tom 08

|

nr 1

9

Analiza polimorfizmu gamma gliadyn w mieszancach Triticum sp. i Aegilops sp.

51%

Tyrka M., Stefanowska G.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

1999

|

nr 211

47-53

10

Molekularna charakterystyka fitoplazm porazajacych roze w Polsce

51%

Sliwa H., Malinowski T., Kaminska M.

Acta Agrobotanica

|

2002

|

tom 55

|

nr 1

325-334

Symptoms of shoot dieback and leaf yellowing followed by leaf chlorosis were observed in naturally infected roses 'Frisco' and 'Suela', cultivated in a commercial greenhouse in Poland. The presence of phytoplasma was demonstrated in affected plants by nested polymerase chain reaction (PCR) with R16Fl/RO and Pl/p7 primer pairs in the first round followed by a second one with RI6F2n/R2, fNrA, Pc399/p1694, R16(1)Fl/Rl and PllfArev primer pairs. Restriction fragment length polymorphism (RFLP) analysis of PCR products (primed with primers R16F2n/R2) was done using enzymes Alul, Msel, Rsal and HpaII. Restriction profiles obtained with these enzymes were identical to those of reference strain AYl belonging to aster yellows phytoplasma group, subgroup l-B (16SrI-B). Nested PCR products from roses 'Frisco' and 'Suela' were sequenced. Analysis of sequences confirmed that the phytoplasma infecting those roses could be classified to aster yellows phytoplasma group, subgroup B.

Ograniczanie wyników

Cross species amplification of coat colour genes in nutrias (Myocastor coypus Mol)

Amplification of the coat protein gene of apple stem pitting virus [ASPV]

Abnormal FHIT gene transcript and c-myc and c-erbB2 amplification in breast cancer

Characterisation of cross-amplified microsatellite markers in the red-breasted flycatcher Ficedula parva

The increase in mitochondrial DNA copy number in the tissues of gamma-irradiated mice

Use of cytochrome b polymorphism for species identification of biological material derived from cattle, sheep, goats, roe deer and red deer

PCR amplification and DNA sequence analysis of parasitic intestinal protozoa in specimens stained with Chlorazol Black E

Evaluation of a novel method based on amplification of DNA fragments surrounding rare restriction sites [ADSRRS-fingerprinting] for typing bacterial strains

Analiza polimorfizmu gamma gliadyn w mieszancach Triticum sp. i Aegilops sp.

Molekularna charakterystyka fitoplazm porazajacych roze w Polsce