Wyniki wyszukiwania

1

Lamellar phase of short amphiphiles: molecular dynamics simulations compared to simple mesoscopic models

100%

Loison C., Schmid F., Mareschal M.

Cellular and Molecular Biology Letters

|

2005

|

tom 10

|

nr Suppl.

2

Lipid and protein composition of exovesicles released from human erythrocytes following treatment with amphiphiles

88%

Hagerstrand H., Isomaa B.

Biophysics of Membrane Transport

|

1994

|

tom 12

|

nr 2

3

Plarosomes, novel efficient phospholipid-amphiphile vesicles for drug delivery

75%

Kozubek A., Gubernator J., Przeworska E., Stasiuk M.

Cellular and Molecular Biology Letters

|

1999

|

tom 04

|

nr 2

4

Effects of bioactive amphiphiles on model cell membranes

75%

Deleu M., Paquot M., Nylander T.

Cellular and Molecular Biology Letters

|

2005

|

tom 10

|

nr Suppl.

5

Monitoring of membrane phospholipid scrambling in human erythrocytes and K562 cells with FM1-43 - a comparison with annexin V-FITC

63%

Wrobel A., Bobrowska-Hagerstrand M., Lindqvist C., Hagerstrand H.

Cellular and Molecular Biology Letters

|

2014

|

tom 19

|

nr 2

The styryl dye FM1-43 becomes highly fluorescent upon binding to cell membranes. The breakdown of membrane phospholipid asymmetry in ionophore-stimulated T-lymphocytes further increases this fluorescence [Zweifach, 2000]. In this study, the capacity of FM1-43 to monitor membrane phospholipid scrambling was explored using flow cytometry in human erythrocytes and human erythrocyte progenitor K562 cells. The Ca2+-dependent phosphatidylserine-specific probe annexin V-FITC was used for comparison. The presented data show that the loss of phospholipid asymmetry that could be induced in human erythrocytes by elevated intracellular Ca2+ or by structurally different membrane intercalated amphiphilic compounds increases the FM1-43 fluorescence two- to fivefold. The profile of FM1-43 fluorescence for various treatments resembles that of phosphatidylserine exposure reported by annexin V-FITC. FM1-43 detected the onset of scrambling more efficiently than annexin V-FITC. The amphiphile-induced scrambling was shown to be a Ca2+-independent process. Monitoring of scrambling in K562 cells caused by NEM-induced Ca2+-release from intracellular stores and by Ca2+ and ionophore A23187 treatment showed that the increase in FM1-43 fluorescence correlated well with the number of annexin V-FITC-detected phosphatidylserine-positive cells. The results presented here show the usefulness of FM1-43 as a Ca2+-independent marker of dissipation in asymmetric membrane phospholipid distribution induced by various stimuli in both nucleated and non-nucleated cells.

6

Do shape transformations in erythrocytes reflect the flip rate of amphiphilic compounds?

63%

Hagerstrand H., Bobrowska-Hagerstrand M., Isomaa B.

Cellular and Molecular Biology Letters

|

1996

|

tom 01

|

nr 1

It has been shown that the time course of echinocyte to discocyte transformation caused by exogenous phospholipids is an accurate measure of the flip rate of the phospholipids in the lipid bilayer [1,2]. In order to explore whether shape changes in erythrocytes are indicative of flip rates of water- soluble amphiphiles, the time course of shape changes caused by a large number of amphiphiles was studied. In case of amphiphiles inducing echinocyte to discocyte or discocyte to stomatocyte transformation it is proposed that the time course of shape transformation may be indicative of the flip rate of the amphiphiles. The relevance of using shape changes in erythrocytes as a tool to estimate flip rates of amphiphiles is discussed.

7

Liposomal drug delivery, a novel approach: PLARosomes

63%

Kozubek A., Gubernator J., Przeworska E., Stasiuk M.

Acta Biochimica Polonica

|

2000

|

tom 47

|

nr 3

Almost from the time of their rediscovery in the 60's and the demonstration of their entrapment potential, liposomal vesicles have drawn attention of researchers as potential carriers of various bioactive molecules that could be used for therapeutic applications in humans and animals. Several commercial liposome-based drugs have already been discovered, registered and introduced with great success on the pharmaceutical market. However, further studies, focusing on the elaboration of more efficient and stable amphiphile-based vesicular (or non-viral) drug carriers are still under investigation. In this review we present the achievements of our group in this field. We have discovered that natural amphiphilic dihydroxyphenols and their semisynthetic derivatives are promising additives to liposomal lipid compositions. The presence of these compounds in lipid composition enhances liposomal drug encapsulation, reduces the amount of the lipid carrier necessary for efficient entrapment of anthracycline drugs by a factor of two, stabilizes liposomal formulation of the drug (both in suspension and in a lyophilized powder), does not influence liposomal fate in the blood circulation system and benefits from other biological activities of their resorcinolic lipid modifiers.

8

Synthetic amphiphiles as non-viral vectors in gene therapy. Development and mechanisms

51%

Hoekstra D., Oberle V., Audouy S., Zuhorn I., Engberts J.

Cellular and Molecular Biology Letters

|

1999

|

tom 04

|

nr 2

9

Elaboration and specific properties of fluorinated liposomes and related supramolecular systems

51%

Krafft M. P., Riess J. G.

Cellular and Molecular Biology Letters

|

1996

|

tom 01

|

nr 4

A range of well defined, pure, highly surface-active but non-hemolyzing fluorinated amphiphiles has recently been synthesized. Fluorocarbon chains strongly enhance the hydrophobic effect that induces the organization of amphiphiles into bilayer membranes, and supplement it with a lipophobic effect as well. This leads to an augmented tendency for fluorinated amphiphiles to selfassemble into vesicles (liposomes), tubules and other supramolecular aggregates when dispersed in water and other solvents. Fluorinated bilayers and vesicles are generally more stable and less permeant than those made from hydrocarbon analogs. The presence of a fluorinated film inside the liposomal membrane also has significant repercussions on the behavior of the liposomes in a biological milieu and on their in vivo recognition.

Ograniczanie wyników

Lamellar phase of short amphiphiles: molecular dynamics simulations compared to simple mesoscopic models

Lipid and protein composition of exovesicles released from human erythrocytes following treatment with amphiphiles

Plarosomes, novel efficient phospholipid-amphiphile vesicles for drug delivery

Effects of bioactive amphiphiles on model cell membranes

Monitoring of membrane phospholipid scrambling in human erythrocytes and K562 cells with FM1-43 - a comparison with annexin V-FITC

Do shape transformations in erythrocytes reflect the flip rate of amphiphilic compounds?

Liposomal drug delivery, a novel approach: PLARosomes

Synthetic amphiphiles as non-viral vectors in gene therapy. Development and mechanisms

Elaboration and specific properties of fluorinated liposomes and related supramolecular systems