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1

The heterogeneity of cell subtypes from a primary culture of human amniotic fluid

100%
Zhang S., Geng H., Xie H., Wu Q., Ma X., Zhou J., Chen F.
Cellular and Molecular Biology Letters
|
2010
|
tom 15
|
nr 3
424-439
Heterogeneous human amniotic fluid contains various cell types. Herein, we report on the possibility of simultaneously isolating three subtypes of cells from one primary culture. Using a stainless steel instrument named a colony poculum, two of the three cell subtypes could be efficiently cultured, and these were further characterized. The results indicated that these two cell subtypes had different morphologies and were characterized by different cell marker expression profiles, including the differential expression of CD105, CD117 and EBAF. Furthermore, their gene expression array data revealed their different gene expression profiles. Although both cell types expressed several embryonic stem cell-specific markers, they were non-tumorigenic in vivo. This paper not only provides new insight into the heterogeneity of human amniotic fluid, it also presents a simple yet efficient cell isolation method. These results will contribute to the thorough investigation of the properties and potential future applications of human amniotic fluid-derived cells.
2

Collagenous constituents of amniotic fluid

100%
Gogiel T., Bielecki D. A., Bankowski E.
Acta Biochimica Polonica
|
1998
|
tom 45
|
nr 4
The amniotic fluid (AF) was fractionated by dialysis, gel filtration and SDS/PAGE, and submitted to the assay of collagenous constituents. The collagenous character of peptides and proteins of amniotic fluid was confirmed by hydroxyproline (Hyp) assay and treatment with bacterial collagenase followed by electrophoresis and gel filtration of the digestion products. It was found that AF contains collagen degradation products but the classical method of Hyp determination described by Woessner (Arch. Biochem. Biophys., 1961, 93, 440-447) gives overestimated values due to the interference with other AF components. Fractionation of AF on Sephadex G-100 column allowed to remove the interfering material and to estimate the actual Hyp content which equals to approx. 6.2 μg/ml. About 70% of Hyp was found in low molecular dialyzable products and the rest (about 30%) appears to be a constituent of non-dialyzable collagenous polypeptides of the molecular mass of about 7.9-26.3 kDa. It is suggested that such collagenous polypeptides may be the products of proteolytic conversion of collagen precursor (procollagen) into the monomeric form of this protein. No high molecular forms of collagen, corresponding to alpha-subunits, were found.
3

Search for the pathogenic fungi in amniotic fluid

100%
Nowakowska D., Wilczynski J., Szaflik K., Kurnatowska A.
Wiadomości Parazytologiczne. Suplement
|
2001
|
tom 47
|
nr 1
4

Concentrations of leptin and neuropeptide Y in maternal plasma, umbilical cord blood and in amniotic fluid in pregnant women with EPH-gestosis

84%
Kokot F., Ulman I., Wiecek A., Irzyniec T., Ulman J.
Archivum Immunologiae et Therapiae Experimentalis
|
1998
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tom 46
|
nr 5
5

A comparative study of the protein C system in mother's blood, cord blood and amniotic fluid

67%
Uszynski M., Uszynski W., Zekanowska E., Kuczynski J., Szymanski M.
Folia Histochemica et Cytobiologica
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2010
|
tom 48
|
nr 2
262-266
6

A comparison of the total antioxidant capacity of some human body fluids

67%
Ziobro A., Bartosz G.
Cellular and Molecular Biology Letters
|
2003
|
tom 08
|
nr 2
The Total Antioxidant Capacity of several human fluids was compared and the following sequence of TAC values was found: urine > saliva > blood plasma > milk ≈ amniotic fluid >> sweat. Lower TAC values were found for the saliva of smokers than for that of non-smokers. Drinking of a cup of instant coffee increased the hydrogen peroxide content of urine but did not decrease the TAC of urine.
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