Wyniki wyszukiwania

1

Crystals of recombinant human tumor necrosis factor

100%

Joachimiak A., Giel M., Klysik J. E., Stec W. J., Barciszewski J.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1991

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tom 39

|

nr 1

2

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Effect of physical and chemical factors in production of alkaline protease enzyme by Bacillus strains

100%

Tebyanian H., Mirhosseiny S. H., Bakhtiari A., Karami A., Dadseresht S., Otroshi B.

International Letters of Natural Sciences

|

2018

|

tom 71

Proteases is family of enzymes and it has crucial role due to their physiological roles and very valuable commercial applications. Alkaline protease are produced by Bacillus species are particular importance because of their thermal stability and stability at different pH values. This study aimed to investigate the effect of physical and chemical factors in production of alkaline protease enzyme fermentation by members of the genus Bacillus. In this study, alkaline protease enzyme production were evaluated in submerged fermentation by Bacillus strains which were isolated from alkaline soils of Guilan province. Factors incubation were optimized such as time, pH, amount of inoculation and ammonium sulfate in alkaline protease enzyme production whit using response surface methodology (RSM) in culture. The maximum enzymatic activity was observed in incubation time of 36 hours, pH=9, inoculation amount of 15% (V) and ammonium sulfate 1.5% (W/V). Factors had significant effect on the production of alkaline protease enzyme such as pH and ammonium sulfate.

3

The influence of non-protein-nitrogen on the growth of rumen ciliate Entodinium caudatum in vitro

84%

Michalowski T., Landa I., Muszynski P., Szczepkowski P.

Acta Protozoologica

|

1987

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tom 26

|

nr 4

4

Optimization of single-cell-biomass production by Yarrowia lipolytica using response surface methodology and pulse method

84%

Musial I., Rymowicz W., Cibis E.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

|

2004

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tom 07

|

nr 1

5

Quality and nutritive value of silage made of meadow sward [3rd cutting] with chemical additives

84%

Rydzik W.

Natural Sciences

|

2000

|

tom 05

6

Analysis of ammonium sulphate toxicity in the freshwater catfish Heteropneustes fossilis using mucocyte indexing

84%

Paul V. I., Banerjee T. K.

Polskie Archiwum Hydrobiologii

|

1996

|

tom 43

|

nr 1

7

Quality and nutritive value of silage made of Persian clover [3rd cutting] with chemical additives

84%

Rydzik W.

Natural Sciences

|

2000

|

tom 05

8

Purification and characterization of extracellular Pseudomonas aeruginosa urate oxidase enzyme

84%

Saeed H. M., Abdel-Fattahyousry Y. R., Gohar M., Elbaz M. A.

Polish Journal of Microbiology

|

2004

|

tom 53

|

nr 1

Urate oxidase (uricase) was isolated and purified from Pseudomonas aeruginosa to apparent homogeneity using ammonium sulphate precipitation followed by ion exchange and gel filtration chromatography. The specific activity of the purified uricase enzyme was found to be 636.36 with the use of uric acid as a substrate. The purified uricase enzyme is a monomeric protein with molecular weight of 64 kilodaltons. The optimal pH and temperature of the purified enzyme is 9.0 and 30°C, respectively. The effect of some metal ions was studied. Sulphate forms of Fe⁺², Zn⁺² and Co⁺² inhibit the uricolytic activity whereas; NaCl and CaCl₂ enhance the enzyme activity. Moreover, the purified enzyme is inhibited by EDTA and KCN.

9

Effect of fertilisation on the content of some manganese fractions in acidic soils

67%

Olek J., Filipek T.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

1998

|

tom 456

10

Purification and characterisation of ferritin from the Baltic blue mussel Mytilus trossulus

67%

Potrykus J., Kosakowska A.

Oceanologia

|

2009

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tom 51

|

nr 4

Baltic blue mussels Mytilus trossulus were collected from the Gulf of Gdańsk (southern Baltic Sea) in order to isolate ferritin from its soft tissues, as well as to purify and characterise this protein. Proteins were isolated from the inner organs of M. trossulus (hepatopancreas, gills and soft tissue residue) by thermal denaturation (70◦C) and acidification (pH 4.5) of the homogenates, followed by ammonium sulphate ((NH4)2SO4) fractionation. The ferritin was then separated by ultracentrifugation (100 000×g, 120 min.). The protein content in the purified homogenates was determined by the Lowry method using bovine serum albumin (BSA) and horse spleen ferritin (HSF) as standards. PAGE-SDS and Western blotting analysis permitted identification of ferritin in the purified preparations. Additionally, the purified homogenates and mussel soft tissue were analysed for their heavy metal contents (especially cadmium and iron) in a Video 11 E atomic absorption spectrophotometer, following wet digestion of the samples (HNO3/HClO4). The electrophoregrams showed that the inner organs of M. trossulus contained ferritin, which, like plant ferritin, is characterised by the presence of subunits in the electrophoregram in the 26.6–28.0 kDa range. The highest ferritin content was recorded in the hepatopancreas, followed by the gills and the soft tissue residue. With regard to the sampling stations, the highest content of ferritin was noted in the animals sampled off Sopot (station D3), and in those collected by a diver off Jastarnia (W1) and Gdynia (W4). Ferritin isolated from the inner organs of mussels collected from these stations also contained the largest quantities of heavy metals (Cd and Fe). Ferritin isolated from the inner organs of mussels collected by a diver from wrecks – sites where the concentrations of iron and other trace metals in the sea water are high – contained higher quantities of heavy metals (Cd and Fe) than the ferritin isolated from the inner organs of mussels collected with the drag. This confirms that ferritin is a protein able to store and transport not only iron, but also, though to a lesser extent, some other heavy metals, including cadmium.

11

The influence of the source of sulphur on its intake by sheep

67%

Chladek G., Kuchtik J., Zapletal D.

Zeszyty Naukowe Akademii Rolniczej we Wrocławiu. Zootechnika

|

2004

|

tom 52

The intake of sulphur offered in different forms was observed in ten ewes of Merino breed during one year. Four sources of sulphur were used: ammonium sulphate, sodium sulphate, calcium sulphate and sulphur. The effect of season (sprihg, summer, autumn and winter) and a phase of reproduction cycle (gestation, lactation, barren period) on the consumption of various sulphur sources were analysed. Significant differences in the intake of sulphur were found in summer, winter (both p<0.01) and autumn (p<0.05) and non-significant differences in spring. A phase of reproduction also affected the intake of sulphur; significant differences were found during gestation, barren period (both p<0.01) and lactation (p<0.05).

12

Ammonification and nitrification in zinc-contaminated soil

67%

Zaborowska M., Wyszkowska J., Kucharski J.

Electronic Journal of Polish Agricultural Universities. Series Agronomy

|

2007

|

tom 10

|

nr 4

13

Changes in the content of sulphur and selected heavy metals in soil fertilised with sulphur

67%

Fryscakova E., Hlusek J.

Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa

|

2007

|

tom 93

231-235

14

Influence of sulphur on the yield and quality of winter oilseed rape

67%

Zukalova H., Matula J., Kuchtova P., Miksik V.

Rośliny Oleiste - Oilseed Crops

|

2001

|

tom 22

|

nr 2

15

Some parameters influencing immunoassay of human and horse myoglobins

67%

Kochanowska I. E., Kuropatwa M., Szewczuk A.

Archivum Immunologiae et Therapiae Experimentalis

|

1997

|

tom 45

|

nr 2-3

16

Isolation and enzymatic properties of pectic enzyme from Fusarium accuminatum

67%

Ghareib M., El-Dein M. M. N.

Acta Microbiologica Polonica

|

1992

|

tom 41

|

nr 1-2

17

Anthropogenic aerosols over the coastal zone of Gdansk Basin

67%

Lewandowska A., Falkowska L.

Archiwum Ochrony Środowiska

|

2005

|

tom 31

|

nr 3

18

Purification and general properties of uricase from Streptomyces aureomonopodiales

67%

Mansour F. A., Nour El-Dein M. M., El-Fallal A. A., Abou Dobara M. I. M.

Acta Microbiologica Polonica

|

1996

|

tom 45

|

nr 1

19

Effect of „anionic salts” on the metabolism of selected minerals and superoxide dismutase activity in blood of cows

59%

Kleczkowski M., Klucinski W., Gajewski Z., Garncarz M., Jakubowski T., Dembele K.

Bulletin of the Veterinary Institute in Puławy

|

2011

|

tom 55

|

nr 1

The aim of this experiment was to evaluate the effect of "anionic salts", such as ammonium chloride (NH₄Cl), aluminium sulphate (Al₂(SO₄)₃·16 H₂O), ammonium sulphate (NH₄)₂SO₄, and calcium chloride (CaCl₂·2H₂O), on the calcium metabolism, copper, zinc, and molybdenum concentrations and superoxide dismutase (SOD) activity in bovine blood. The experiment was carried out on 26 cows over a 2 year period. Cattle were fed daily fodder containing the chemical compounds. During the first year, two districts (A - experimental, B - control) were evaluated for deficiency diseases. During the second year, blood concentrations of calcium, phosphorus, magnesium, copper, zinc, molybdenum, and alkaline phosphatase (ALP) and SOD activity were evaluated. Urine pH was also determined. Before and after supplementation, the dietary cation anion difference (DCAD) was evaluated and a mineral fodder ration was formulated for the cows. There were significant differences in the concentration of chlorine and sulfur in the fodder of cows between group A and B after supplementation. Mineral supplementation with chlorides and sulphates is particularly advantageous for systemic acidification. It consisted of the occurrence of lower pH and higher blood concentration of calcium and copper and higher activity of SOD in the urine of experimental cows. This can be seen as improvement of calcium metabolism indicators. DCAD balance was reduced from +241.5 mmol/kg DM to -79.5 mmol/kg DM diet. An increase in ALP activity to normal levels was seen when the animals were given chlorides and sulphates. The study showed that "anionic salts" had a positive effect on mineral metabolism and antioxidant status of dairy cows.

20

Optimization of cultivation medium composition of an L-lysine producing mutant: the use of response surface methodology

59%

Udeh K. O., Achremowicz B.

Acta Microbiologica Polonica

|

1993

|

tom 42

|

nr 2

Ograniczanie wyników

Crystals of recombinant human tumor necrosis factor

Effect of physical and chemical factors in production of alkaline protease enzyme by Bacillus strains

The influence of non-protein-nitrogen on the growth of rumen ciliate Entodinium caudatum in vitro

Optimization of single-cell-biomass production by Yarrowia lipolytica using response surface methodology and pulse method

Quality and nutritive value of silage made of meadow sward [3rd cutting] with chemical additives

Analysis of ammonium sulphate toxicity in the freshwater catfish Heteropneustes fossilis using mucocyte indexing

Quality and nutritive value of silage made of Persian clover [3rd cutting] with chemical additives

Purification and characterization of extracellular Pseudomonas aeruginosa urate oxidase enzyme

Effect of fertilisation on the content of some manganese fractions in acidic soils

Purification and characterisation of ferritin from the Baltic blue mussel Mytilus trossulus

The influence of the source of sulphur on its intake by sheep

Ammonification and nitrification in zinc-contaminated soil

Changes in the content of sulphur and selected heavy metals in soil fertilised with sulphur

Influence of sulphur on the yield and quality of winter oilseed rape

Some parameters influencing immunoassay of human and horse myoglobins

Isolation and enzymatic properties of pectic enzyme from Fusarium accuminatum

Anthropogenic aerosols over the coastal zone of Gdansk Basin

Purification and general properties of uricase from Streptomyces aureomonopodiales

Effect of „anionic salts” on the metabolism of selected minerals and superoxide dismutase activity in blood of cows

Optimization of cultivation medium composition of an L-lysine producing mutant: the use of response surface methodology