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Does the membrane potential control incorporation of tubulovesicles into the secreting apical membrane of the rat parietal cell?

100%

Ostrowski J., Dolowy K., Zych W., Butruk E.

Journal of Physiology and Pharmacology

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1991

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tom 42

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nr 4

The present studies were designed to examine the effect of changes in membrane potential by means of protonophore carbonyl cyanide m-chlorophenylhydrazone (СССР) and variations in the pH of the medium on the secretory response of parietal cells. Studies were performed in vitro using isolated cells from rat stomachs and acid production was indirectly determined by ¹⁴C-aminopyrine (AP) accumulation. СССР affected both basal and histamine-stimulated AP accumulation in a concentration-dependent manner. The AP accumulation ratios depended on pH of the incubation medium; the ratio was lowest at pH 6.6, and increased progressively as the pH of the medium increased to 7.8. Moreover, the decreases in AP accumulation ratios caused by simultanous addition of СССР and AP to cell suspensions compared to those in which СССР was added to incubated cells after achieving the steady-state of AP accumulation were quantitatively similar. These findings suggest that the decrease in AP accumulation due to СССР treaiment is a consequence of an activation of acid secretion rather than an inhibitor of acid production. From the present and previously published data, we propose a working hypothesis: membrane recycling is dependent on changes in apical membrane potential.

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The relationship between extracellular K-plus and Ca2-plus on aminopyrine accumulation in rat parietal cells

86%

Ostrowski J., Zych W., Dolowy K., Butruk E.

Journal of Physiology and Pharmacology

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1991

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tom 42

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nr 3

The effects of extracellular K⁺ in relation to extracellular Ca²⁺ on acid production were studied. Studies were performed in vitro using isolated cells from rat stomachs, and acid production was indirectly determined by ¹⁴C-aminopyrine (AP) accumulation. In the absence of K⁺ in the incubation medium histamine-stimulated AP accumulation ratios were significantly decreased independently in the presence or absence of extracellular Ca²⁺. Under basal conditions, in the absence of extracellular Ca²⁺ , increasing concentrations of extracellular K⁺ enhanced AP accumulation ratios to significantly higher than those found in the presence of Ca²⁺. In histamine-, cAMP-, and carbachol-stimulated parietal cells, high K⁺ concentrations increased AP accumulation significantly less in Ca²⁺-free than in Ca²⁺-containing media. High K⁺ also induced significantly both an increase in cytosolic free Ca²⁺ concentration and ⁴⁵ Ca²⁺ uptake. The present results confirmed the importance of K⁺ in gastric acid production and suggested a role for Ca²⁺ as a modulator of mechanisms of parietal cell stimulation.

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Functional and structural changes of isolated rat parietal cells during membrane potential modulation

72%

Ostrowski J., Jarosz D., Zych W., Wojciechowski K.

Journal of Physiology and Pharmacology

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1994

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tom 45

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nr 3

4

Helicobacter pylori stimulates gastric acid secretion via platelet activating factor

58%

Sobhani I., Bado A., Cherifi Y., Moizo L., Laigneau J. P., Pospai D., Mignon M., Lewin M. J.

Journal of Physiology and Pharmacology

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1996

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tom 47

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nr 1

Ograniczanie wyników

4 Journal of Physiology and Pharmacology

3 Ostrowski J.

3 Zych W.

2 Butruk E.

2 Dolowy K.

1 Bado A.

1 Cherifi Y.

1 Jarosz D.

1 Laigneau J. P.

1 Lewin M. J.

1 Mignon M.

1 Moizo L.

1 Pospai D.

1 Sobhani I.

1 Wojciechowski K.

1 1996

1 1994

2 1991

Does the membrane potential control incorporation of tubulovesicles into the secreting apical membrane of the rat parietal cell?

The relationship between extracellular K-plus and Ca2-plus on aminopyrine accumulation in rat parietal cells

Functional and structural changes of isolated rat parietal cells during membrane potential modulation

Helicobacter pylori stimulates gastric acid secretion via platelet activating factor