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This paper reports on amino acid profiles of the flesh of heterosexuals of porcellanids collected from the Atlantic Ocean at Orimedu beach in Ibeju-Lekki, Lagos, Nigeria. Results showed that high values of amino acids were observed in the heterosexual flesh of Neopetrolisthes maculatus (g 100g⁻¹ protein): 17.7 – 17.8 (Glu), 9.90 – 10.0 (Asp), 8.70 – 9.07 (Arg), 7.23 – 7.94 (Leu) and 5.81 – 6.06 (Gly). Total essential amino acid values ranged from 45.2 to 46.2 g 100⁻¹ g. Predicted protein efficiency ratio was 3.82 – 4.14, the range of essential amino acid index was 86.9 – 89.9, the biological values ranged from 83.0 to 86.3. The Lys/Trp was 3.31- 4.27. Serine was limiting amino acid (0.513 – 0.516) in the egg score comparison; under the essential amino acids scores, Lys (0.840) was limiting in female but Val (0.823) was limiting in the male; Lys (0.796 – 0.905) was limiting in both samples in the pre-school child amino acid requirements. It was observed that out of the twenty parameters determined, male flesh was more concentrated in 60% values than the female flesh and 40% better in female than male. Correlation coefficient result showed that significant differences existed in the amino acids composition at r = 0.01 of the N. maculatus samples.
This article reports the amino acid profiles of the innards of the male and female dry samples of Neopetrolisthes maculatus collected from the Atlantic Ocean at Orimedu beach in Ibeju-Lekki, Lagos, Nigeria. The analytical results showed high values of amino acids being observed in both heterosexual samples (g 100g⁻¹ protein): 8.17-8.32 (Leu), 8.35-10.3 (Asp), 17.6-18.2 (Glu) and 7.76-9.55 (Arg) with total amino acid values being greater in female innards (97.6g100g⁻¹) than the male innards (95.5g 100g⁻¹). These quality parameters were instructive of the quality of the amino acids in the innards of N. maculatus: P-PER1, (2.83-3.01), P-PER2 (2.89-2.96), EAAI (88.7-89.0), BV (85.0-85.5), Lys/Trp (L/T) (3.00-5.01), Met/Trp (M/T) (1.78-3.50) and Phe/Tyr (1.04 - 1.65). The pI values were close at 5.46-5.57. In the amino acid groups (classes), the following trend was observed: class I > IV >V > VI > II > III > VII. For the amino acid scores: serine (0.487-0.511) was limiting in both samples on the total hen’s egg scoring pattern; in provisional scoring pattern, Lys was limiting in both samples with values of 0.820-0.889 and in the pre-school amino acid requirements, Lys was also limiting at 0.778-0.843. In the statistical analyses total amino acid profiles as well as egg scores were significantly different between the two samples whereas quality scores in pre-school amino acid requirements and provisional amino acid scoring pattern were both not significantly different between the two samples all at r=₌₀.₀₁. Among the EAAs, six out of nine (66.7%) were more concentrated in the male innards and three of nine (33.3%) were more concentrated in the female. Thus the overall summary showed the male innards amino acids were of better quality than in the female as shown: male innards EEA = 46.1 g100g⁻¹ and 46.0 g100g⁻¹ in the female with corresponding TNEAA of 49.3g 100g⁻¹ and 51.6g 100g⁻¹ respectively.
The impact of puffing on nutritional composition and phenolic profiles of kiwicha (Amaranthus caudatus L.) and quinoa (Chenopodium quinoa Willd.) was investigated. Popped kiwicha showed increased protein and lipid contents and lower contents of carbohydrates compared to the untreated grains. Higher lipid, ash and carbohydrates contents and a decreased protein content were observed after puffing of quinoa. Fatty acid profile and ω-6/ω-3 ratio was not affected by puffing, although it was observed a healthier ratio in quinoa (7:1) compared to kiwicha (65:1). Thermal treatment reduced essential amino acid contents and protein quality of both grains, although amino acids content remained adequate according to FAO/WHO requirements for adults. puffing decreased hydroxybenzoic and hydroxycinnamic acids content of both pseudocereals. Flavonoid levels were negatively affected by puffing in kiwicha while a noticeable increase was observed in popped quinoa. In summary, puffing of kiwicha and quinoa grains is an alternative processing method to obtain expanded products or precooked flours of adequate nutritional value.
The nutritional composition and amino acid profile of Moina macrocopa were studied using different types of yeast (Saccharomyces cerevisiae and Rhodotorula glutinis) as fodder substrates. The effective accumulation of carotenoids in Moina macrocopa during R. glutinis yeast application was not accompanied by deterioration in the nutritional value of zooplankton. The content of total proteins and total lipids in Moina grown on S. cerevisiae and R. glutinis was not significantly different. However, the use of R. glutinis in the cultivation of M. macrocopa led to the change in the ratio of proteinogenic amino acids in the studied cladocerans. In particular, the share of methionine, leucine and isoleucine significantly increased. It allowed to enhance the quality of protein in the fodder zooplankton, that is especially important in the feeding of fish fry.
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