Seed mass is a critical lifehistory character in seed evolutionary ecology. Plant species can present responses in seed mass to environment stresses. We tested the hypotheses that seed mass was positively correlated with altitude within species. We selected four congeneric Saussurea species as study objects, and collected their seeds along altitudinal gradients (2100–4200 m a.s.l.) in the alpine area of the Qinghai-Tibetan Plateau, China. Results showed that mean seed mass of the four species were significantly affected by altitude (P <0.001). There was a general trend of an increase in seed weight with altitude among the populations of the four species. In addition, mean seed mass of four species were not significantly different, but all presented a bigger coefficients of variation within species along altitude gradients. Our results indicate selection pressure within species, with larger seeds occurring at higher altitudes.