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Voltage activated ion channels formed by a synthetic alpha-helical peptide: - Determination of channel stoichiometry and molecular modelling of peptide conformation

100%

Mellor I. R., Kerr I. D., Sansom M. S. P., Sukumar M., Balaram P.

Cellular and Molecular Biology Letters

1996

tom 01

nr 3

We designed and synthesized an 18 residue peptide (MS 18) similar to the channel forming fungal antibiotic alamethicin. MS18 formed ion channels in lipid bilayers exhibiting a low discrete conductance level of 55 pS and brief openings to many other conductance levels. Channel formation was markedly dependent on transbilayer voltage with macroscopic conductance increasing exponentially beyond an activation voltage. The activation voltage was higher for lower concentrations of peptide. The relationship between conductance, voltage and peptide concentration was used to calculate the mean number of peptide monomers forming the MS18 channel. This gave an estimate of 4 MS18 monomers per channel. Molecular modeling of MS18 revealed a predominantly α-helical structure.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Balaram P.

1 Kerr I. D.

1 Mellor I. R.

1 Sansom M. S. P.

1 Sukumar M.

1 1996

Wyniki wyszukiwania

Voltage activated ion channels formed by a synthetic alpha-helical peptide: - Determination of channel stoichiometry and molecular modelling of peptide conformation