Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 93

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 5 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  alkaline phosphatase
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 5 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Remediation of soil contaminated with cadmium

86%
The search for the best solutions to restore soil balance is essential for attaining a stable and sustainable agricultural development worldwide. This research, which makes a contribution to these investigations, focuses on four substances (two innovative ones: basalt flour, brown algal extract, and two classic soil improvers: finely ground barley straw and compost) which can potentially alleviate the inhibitory effect of Cd2+ on the soil environment. The following were analyzed: the activity of acid phosphatase and alkaline phosphatase, counts of Pseudomonas sp., cellulolytic bacteria, copiotrophic bacteria and copiotrophic spore-forming bacteria, and the yield of spring barley. Cadmium (Cd2+) was applied as CdCl2 · 2.5H2O in the following doses: 0, 4, 40, 80, 120, 160, and 200 mg Cd2+ kg-1 of soil. For a more complete assessment of the soil, its biochemical properties and the counts of microorganisms were scrutinized with the following indices: RS – soil resistance, R:S – rhizosphere effect and EF – fertilization effect of the contamination alleviating substances. It was found that alkaline phosphatase is more sensitive to cadmium contamination of the soil than acid phosphatase. Cadmium did not exert any inhibitory effect on the number of microorganisms present or the yield of spring barley. Cellulolytic bacteria were the least sensitive to stress associated with the accumulation of high cadmium doses in the soil, whereas copiotrophic bacteria were the most sensitive microorganisms to the above stressor. The ability of cadmium-polluted soil to restore homeostasis depended on the type of a soil improver and the level of soil contamination. Negative consequences of cadmium pollution were effectively mitigated by straw, but less so by brown algal extract and basalt flour.
The aim of the study has been to determine the effect of soil contamination with zinc on the activity of soil enzymes. The study consisted of two laboratory experiments. Same, light loamy soil of pH 7.1, was used in both experiments. The variables in the first experiment were: a degree of soil contamination with zinc in mg Zn kg-1 d.m. of soil: 0, 5, 500, 1000, 1500 and 2000; dose of cellulose in g kg-1 d.m. of soil: 0, 15, and time of soil incubation (15 – 120 days). In the second experiment the following variables were tested: the degree of soil contamination with zinc in mg Zn kg-1 d.m. of soil: 0, 1000 and 2000; soil pH: 7.1, 6.4 and 5.5, and the time of soil incubation (15 – 120 days). The results of the experiments demonstrated that contamination of soil with zinc led to depressed activity of dehydrogenases, urease, acid phosphatase and alkaline phosphatase. Dehydrogenases and urease appeared to be more vulnerable to zinc contamination than phosphatases. The soil enzymes were adversely affected not only by zinc contamination but also by increasing soil acidity. According to their vulnerability to soil acidity the soil enzymes can be ordered as follows: dehydrogenases > urease > alkaline phosphatase > acid phosphatase. Cellulose added to soil (15 g kg-1) proved to be a good factor in the improvement of soil biochemical properties, although it did not limit the effects produced by zinc.
In this review the roles of specific proteins during the first step of mineralization and nucleation are discussed. Mineralization is initiated inside the extracellular organelles–matrix vesicles (MVs). MVs, containing relatively high concentrations of Ca2+ and inorganic phosphate (Pi), create an optimal environment to induce the formation of hydroxyapatite (HA). Special attention is given to two families of proteins present in MVs, annexins (AnxAs) and tissue-nonspecific alkaline phosphatases (TNAPs). Both families participate in the formation of HA crystals. AnxAs are Ca2+- and lipid-binding proteins, which are involved in Ca2+ homeostasis in bone cells and in extracellular MVs. AnxAs form calcium ion channels within the membrane of MVs. Although the mechanisms of ion channel formation by AnxAs are not well under-stood, evidence is provided that acidic pH or GTP contribute to this process. Furthermore, low molecular mass ligands, as vitamin A derivatives, can modulate the activity of MVs by interacting with AnxAs and affecting their expression. AnxAs and other anionic proteins are also involved in the crystal nucleation. The second family of proteins, TNAPs, is associated with Pi homeostasis, and can hydrolyse a variety of phosphate compounds. ATP is released in the extracellular matrix, where it can be hydrolyzed by TNAPs, ATP hydrolases and nucleoside triphosphate (NTP) pyrophosphohydrolases. However, TNAP is probably not responsible for ATP-dependent Ca2+/phosphate complex formation. It can hydrolyse pyrophosphate (PPi), a known inhibitor of HA formation and a byproduct of NTP pyrophosphohydrolases. In this respect, antagonistic activities of TNAPs and NTP pyrophosphohydrolases can regulate the mineralization process.
 Lansoprazole, a known H+/K+-ATPase inhibitor, is currently used as a therapeutical option for the initial treatment of gastroesophageal reflux disease. Recently, lansoprazole has been found to be an inhibitor of cytosolic PHOSPHO1 (a phosphatase which hydrolyses phosphocholine and phosphoethanolamine), providing a possible therapeutical target to cure pathological mineralization. Since PHOSPHO1 is present inside matrix vesicles, we tested the effect of lansoprazole on matrix vesicles containing several key enzymes for the mineralization process including tissue-nonspecific alkaline phosphatase. We found that lansoprazole can inhibit in an uncompetitive manner tissue-nonspecific alkaline phosphatase. A Ki value of 1.74 ± 0.12 mM has been determined for the inhibition of tissue-nonspecific alkaline phosphatase by lansoprazole. Lansoprazole, currently used for treating gastroesophageal disease, by inhibiting PHOSPHO1 and tissue-nonspecific alkaline phosphatase could prevent hydroxyapatite-deposition disease and could serve as an adjunct treatment for osteoarthritis.
Assessment of soil phosphatase activity, phosphorus and heavy metals content depending on the mineral fertilization. The paper presents the results of research into the activity of alkaline and acid phosphatase, the content of available phosphorus, heavy metals and total organic carbon, against in soil with mineral fertilization only. The first experimental factor was phosphorus, potassium, magnesium, calcium and sulphur fertilization in six fertilizer combinations: 1 – PKMgCaS, 2 – KMgCaS, 3 – PMgCaS, 4 – PKCaS, 5 – PKMgS, 6 – PKMgCa. The second factor was made up of nitrogen fertilization at the rates of: 0, 50, 100, 150, 200, 250 kg·haˉ¹ of N. Increasing nitrogen rates and a lack of liming increased the soil acidity inhibiting alkaline phosphatase, decreasing the content of available phosphorus in soil. A lack of phosphorus fertilization resulted in an intensive increase in the activity of both alkaline and acid phosphatase in soil. Due to the experimental factors applied, the content of the heavy metals assayed was as follows: zinc > copper > lead > cadmium.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 5 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.