The aim of the present study was to verify the feasibility of using live Artemia salina nauplii embedded with fluorochromes for the mass marking of pike Esox lucius (L.) larvae. In the experiment, pike larvae 6 days post hatch were fed ad libitum with nauplii dyed with 600 ppm tetracycline hydrochloride (TC) or 200 ppm alizarin red S (ARS) for 3 or 6 days. The highest percentage of marked fish (100%) and the best quality of this marking was found in the groups fed A. salina stained with TC for either 3 or 6 days. In groups fed A. salina stained with ARS for 3 or 6 days exhibited a lower percentage of marked fish (ranging from 76.7–88.3%). No significant differences between experimental groups were noted regarding survival rate, final body weight and length of the reared pike larvae.