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The aim of the present study was to evaluate the degree of microbial air contamination in three laboratory rooms designed for raising broiler chickens under identical conditions, in the summer and winter. It was found that in identical poultry houses and under identical management conditions, certain differences can be observed with regard to temperature and humidity parameters and the degree of microbial air contamination, both in the summer and winter. The concentrations of aerobic mesophilic bacteria and fungi were higher in the winter than in the summer in all rooms. Various levels of microbial air contamination had no effect on broiler production results.
The main microclimate parameters, i.e. bacterial count and airborne emission to the immediate environment, were analyzed in a dairy barn. Air temperature, relative humidity and air flow velocity were measured on an attested Testo 400 device (Testo Inc., Germany). Air samples were collected by use of a Merck MAS-100 device (Merck KgaA, Darmstadt, Germany) onto a commercially available nutrient Columbia agar (Biolife, Milan, Italy) and incubated for 24 h in an incubator at 37oC work temperature. Measurements were carried out once a week in the morning, at noon, and in the evening during October and November 2002. In the barn, measurements were performed in the animal housing area along the feedlot, and outside the barn at a distance of 5 m, 25 m and 50 m eastward and westward from the barn. The measured dairy barn temperature ranged from 11.2oC to 13.1oC, relative humidity from 71.3-78.6%, and air flow velocity from 0.09-0.11 m/s. The mean value of total bacterial count in the barn air ranged from 2.82 x 104 cfu/m3 at noon to 7.76 x 104 cfu/m3 in the evening. Bacterial count decreased at particular measuring sites outside the barn, with Wilcoxon matched pair test showing statistical significance (p<0.05) at a distance of 5 m eastward and 5 m westward of the barn.
Since intensive poultry production is accompanied by as high as possible densities of birds within buildings, this exposes poultry house workers to elevated concentrations of bioaerosol that is mainly emitted by birds. Exposure to dust containing pathogenic microbial and parasitic agents may cause asthma, asthma-like syndrome, mucous membrane irritation, chronic bronchitis, and allergic alveolitis organic dust toxic syndrome, as well as chronic obstructive pulmonary diseases. Since the microbial air pollution data base of poultry houses is insufficient at present, and poultry production is increasingly widespread, it is important to collect, compare and update the available data.
A study on indoor air microbiological contamination in various rooms of university buildings in Poznań, Poland, is presented. Investigations were conducted in the period September-October 2002 and the same period in 2003. Air samples were taken twice a day: in the morning and in the afternoon. In all of the tested places a multiple growth of bacteria and significant increase of mould spores was observed in afternoons. The predominant bacteria and moulds isolated from investigated air samples were: Staphylococcus spp., Micrococcus spp., Serratia spp., Aspergillus spp., Penicillium spp., Rhizopus spp., Cladosporium spp. and Alternaria spp. Among these microbes the presence of pathogenic and strongly allergenic microorganisms was detected.
The concentration levels of airborne bacteria were measured in clinical/hospital rooms in Upper Silesia, Poland, in buildings of varying conditions. It was found that the typical level of bacterial aerosol concentration is about 10³ CFUm⁻³ in clinical outpatient rooms and ranges from 10² CFUm⁻³ to 10³ CFUm⁻³ in hospitals, depending on the number of occupants and physical quality of the building. The increased level of the airborne bacteria in patient rooms resulting from bed-making was noticed. The Staphylococcus/Micrococcus group was a dominating part of the bacteria in studied hospitals/clinic air, contributing together 58-78% of the total bacteria concentration, confirming that detected airborne bacteria mainly originated from human organisms. The size distributions of bacterial aerosol in naturally ventilated rooms have peaks in the size range between 1.1 and 3.3 μm while in the mechanically ventilated hospital rooms with HVAC the peak appears in the diameter range from 3.3 μm to 4.7 μm.
A novel biofi lter containing organic, bentonite and halloysite media was applied for elimination of microbial pollutants from the air of an industrial hatchery. The concentrations of total mesophilic bacteria, Gram-negative bacteria, thermophilic actinomycetes, dust and bacterial endotoxin were determined in the air of hatchery during 2 months before installation of the biofi lter, and during 6 months after installation of the biofi lter, at the inlet and outlet ducts from each medium. Before installation of the biofi lter, the concentrations of total mesophilic bacteria, Gram-negative bacteria, thermophilic actinomycetes, dust and endotoxin in the air were within the ranges of 0.97- 131.2 × 103 cfu/m3, 0.0-34.4 × 103 cfu/m3, 0.0-0.02 × 103 cfu/m3, 0.37-4.53 mg/m3, and 50.9-520,450.4 ng/m3, respectively. Enterococcus faecalis and Gram-negative bacteria (Acinetobacter spp., Escherichia coli, Enterobacter cloacae, and other species) prevailed among bacterial species recovered from the air of the hatchery. A total of 56 species or genera of bacteria were identifi ed in the air samples taken in the examined hatchery; of these, 11, 11 and 6 species or genera respectively were reported as having allergenic, immunotoxic and/or infectious properties The concentrations of total mesophilic bacteria, Gram-negative bacteria, Enterococcus faecalis and endotoxin found at the inlet duct of the biofi lter after its installation were signifi cantly smaller compared to those recorded before its installation (p<0.05). The concentrations of Gram-negative bacteria, Enterococcus faecalis and dust found at the outlet ducts of biofi lter after its installation were signifi cantly smaller compared to those recorded at the inlet duct of the biofi lter (p<0.01). The concentrations of total meso-philic bacteria were also smaller at the outlet ducts of the biofi lter compared to that at the inlet duct; however, the difference was not signifi cant because of the massive growth of Streptomyces species in the biofi lter’s media which contaminated the outcoming air. In conclusion, the applied biofi lter proved to be effective in the elimination of potentially pathogenic bacteria, dust and endotoxin from the air of the hatchery. The effi cacy of the biofi lter could be improved by the inhibition of the Streptomyces growth in the media of the biofi lter.
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