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1

Genotoxicity of various agents evaluated by a comet assay [critical approach]

100%
Cebulska-Wasilewska A., Wiechec A., Dyga W., Panek A., Pawlyk I.
Folia Histochemica et Cytobiologica. Supplement
|
2001
|
tom 39
|
nr 1
2

Rapid urban growth in the Qazvin region and its environmental hazards: implementing an agent-based model

75%
Hosseinali F., Alesheikh A. A., Nourian F.
Polish Journal of Environmental Studies
|
2014
|
tom 23
|
nr 3
Urban growth is a prevalent challenge in many countries as it causes unexpected changes in land-uses of surrounding areas of cities and endangers the environment and natural resources. Thus, spatial planners and environmental managers always look for the models that simulate the expansion of urban land-use, and enable them to prevent unbalanced expansion of cities, and guide the developments to the desired areas. Several methods have been devised to simulate the dynamics of land-use development. However, the complexity of urban growth is recognized as a major barrier for such simulation methods. Agent-based models as a dynamic bottom-up approach use the real actors of land-use development as their basic components. Thus, such models have found popularity in simulating land-use development and urban sprawl modeling. This paper introduces a new agent-based model used for simulating urban land-use development in our study area located in Qazvin province, Iran. The orchards that encompass the western, eastern, and southern sides of Qazvin city are the most sensitive zones in the study area. The model uses 2005 data for the purpose of calibration and 2010 data for the goal of evaluation. A Kappa accuracy of 82.78% was finally achieved for the predication of the observed developments. Also, three zones of residential developments around Qazvin city were found to be endangered. Orchards located on the eastern side of Qazvin city are exposed to destruction and conversion into urban areas. The calibrated model can also be used as a useful tool for predicting future land-use developments and for recognizing endangered environmental zones.
3

Co-inoculation with two non-infectious cDNA copies of potato spindle tuber viroid [PSTVd] leads to the appearance of novel fully infectious variants

75%
Podstolski W., Gora-Sochacka A., Zagorski W.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 1
Potato spindle tuber viroid (PSTVd) is one of the smallest (about 360 nt) infectious plant agents. It is composed of a single-stranded circular non-coding RNA molecule. In the course of previous passage experiments with two intermediate PSTVd variants I2 and I4, three non-infectious clones (I2-50, I4-37 and I4 VI-17) were found. When inoculated separately as cDNAs on tomato “Rutgers” test plants these variants did not induce any visible disease symptoms and did not produce progeny. The presence of such non-infectious variants raises several questions about their origin and biology and to answer them, mixed co-infections with cDNA copies of two non-infectious variants (I2-50, I4-37) were performed. PSTVd infection was observed in seven out of 30 inoculated plants. The progeny isolated from three separate plants contained novel variants, together with the parental I2 and I4 sequences. It is conceivable that the appearance of repaired PSTVd molecules, clearly capable of cell-to-cell movement leading to the systemic infection, results from recombination events. An analysis of the recombinant molecules and comparison with databases identified the specific sites responsible for the restricted infectivity of the I2-50 and I4-37 PSTVd variants. In parallel experiments in which (+) strand PSTVd infectious transcripts were used, no recombinants were observed, and the original I2-50 and I4-37 non-infectious sequences were not detected in the progeny.
4

Non-glycerol based lipofectins for gene delivery

75%
Rao N. M., Chaudhuri A.
Cellular and Molecular Biology Letters
|
2002
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tom 07
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nr 2
5

In vitro activity of caspofungin against planktonic and sessile Candida sp. cells

75%
Serefko A., Chudzik B., Malm A.
Polish Journal of Microbiology
|
2006
|
tom 55
|
nr 2
Candida sp. may be regarded as one of the leading etiologic agents of hospital-acquired infections, including those related with the indwelling medical devices, which become colonized by the yeasts, accompanied by biofilm formation. In this paper we assayed in vitro susceptibility to caspofungin of planktonic and sessile cells of nasopharyngeal isolates of Candida sp. Two types of biomaterials were used - silicone elastomer-coated latex urinary Foley catheter and PCV Thorax catheter. The minimal inhibitory concentrations (MIC) of caspofungin for planktonic Candida sp. cells ranged from 0.008 to 0.031 mg/l, while the minimal fungicidal concentrations (MFC) from 0.008 to 0.062 mg/l, with MFC/MIC ratios ≤2. The minimal concentration of caspofungin preventing adhesion process of Candida sp. on both biomaterials ranged from 0.004 to 0.031 mg/l, while preventing biofilm formation from 0.004 to 0.062 mg/l. In contrast, much higher minimal concentrations of caspofungin were needed to eradicate the mature biofilm (0.25 to >8 mg/l). In all cases, drug concentrations depended on the strain and the biomaterial used. Our preliminary data suggest that caspofungin, showing good anti-adherent activity in vitro against Candida sp., appears to be a potential agent rather for prophylaxis of the yeast infections associated with biomaterials but not for their treatment.
6

Effect of KLP-602 on virus replication in cell cultures

63%
Malaczewska J., Rotkiewicz Z.
Polish Journal of Veterinary Sciences
|
2004
|
tom 07
|
nr 2
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Screening of Polish, other European and tropical isolates of Verticillium chlamydosporium to assess their potential as biological control agents of root-knot nematodes

63%
Sosnowska D., Mauchline T. H., Bourne J. M., Kerry B. R.
Journal of Plant Protection Research
|
2001
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tom 41
|
nr 1
Three Polish, four other European and three tropical isolates of the nematophagous fungus Verticillium chlamydosporium were screened for their ability to colonise the surface of barley roots, produce chlamydospores and infect eggs of Meloidogyne incognita in laboratory tests. PCR-fingerprinting of different Polish isolates and Vc10 was used to detect differences between isolates. Among the European isolates, the Polish isolate which came from a field fertilised with manure produced the greatest number of viable chlamydospores, the largest proportion of infected eggs of Meloidogyne incognita and the most prolific root colonisation. Among the tropical isolates, an isolate A produced the largest number of chlamydospores and an isolate B was the most prolific root coloniser and parasitised most of M. incognita eggs.
8

Biochemical characterization of surface antigens of infective larvae and adults of Haemonchus contortus

63%
Schallig H. D. F. H., Baczkowska A. M., Wedrychowicz H.
Acta Parasitologica
|
1995
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tom 40
|
nr 4
Surface proteins of the nematode Haemonchus contortus were biochemically characterized and their antigenic properties were investigated. Living parasites were labelled with NHS-biotin. Next, extracts were made using cetyltrimethylammonium bromide (СТАВ), Tris buffered saline (TBS), sodium dodecyl sulphate (SDS) and 2-mercaptoethanol (BME) as solubilizing agents. The presence of glycoproteins in the extracts was examined by probing Western blots with the lectin Concanavalin A (Con A). The antigenic character of the surface proteins was studied by immunoblotting. Glycosylated polypeptides constituted the major part of the surface molecules of larvae, whereas the proportion of glycoproteins in adults is relatively smaller. Surface biotinylation of intact nematodes revealed that СТАВ-, TBS- and SDS-soluble proteins belong to the epicuticle of larvae. СТАВ- and TBS-soluble proteins are present in the surface of adult worms. Many surface proteins of H. contortus were recognized by hyperimmune sera. It was found that the majority of these antigens possess carbohydrate epitopes. However, antigens with nonsugar epitopes are also present in the epicuticle.
9

A comparison of the economic effectiveness of various spawning agents for stimulating the reproduction of the cultured and wild forms of the common barbel Barbus barbus (L.)

51%
Hakuc-Blazowska A., Kupren K., Turkowski K., Targonska K., Zarski D., Kucharczyk D.
Polish Journal of Natural Sciences
|
2010
|
tom 25
|
nr 3
The objective of this study was to evaluate the economic effectiveness of various hormonal products for stimulating the reproduction of the cultured and wild forms of the common barbel Barbus barbus (L.). Three hormonal products were analyzed: Ovopel, Ovaprim (cultured and wild fish) and hCG (cultured fish). The economic effectiveness of hormonal stimulants was analyzed in view of ovulation rates, the price of hormonal products and spawner mortality rates. The cost of hormonal injection per 1000 eggs and 1000 hatchlings was adopted as an indicator of economic effectiveness. The best results were reported for hormonal stimulation using synthetic GnRH analogues during induced spawning of the barbel in a group of cultured fish. The above product’s effectiveness in the controlled spawning of cyprinids is demonstrated by high ovulation rates and high embryo survival rates.
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Proposal for a method for testing resistance of clothing and gloves to penetration by pesticides

51%
Krzeminska S., Szczecinska K.
Annals of Agricultural and Environmental Medicine
|
2001
|
tom 08
|
nr 2
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