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The aim of these studies was to assess the effect of chemical conservants (FA = formic acid, PA = propionic acid, FPA+i = formic acid, propionic acid and ammonium ions) on the cell counts of yeasts and mould fungi in silage. The silage was prepared from corn (Zea mays L), cultivar Buran FAO (240). The effect of the applied conservants on silage aerobic stability was also assessed. The performed chemical analyses comprised the determination of: the content of dry matter (DM), lactic acid (LA), acetic acid (AA), ethanol, water soluble sugars (WSC), crude protein (CP) and pH. The applied preparations were found to reduce the number of yeast and mould fungi cells in all the examined silages. The growth of fungi was inhibited most strongly by the FPA+i preparation (containing a mixture of formic and propionic acids and ammonium ions). The yeast cell counts dropped (P«0.05) from 8.50 107 CFU g-1 silage in the control (CCS = control corn silage) to 2.60 107 CFU g' in silage treated with FPA+i, whereas counts of the mould fungi cells - from 15.20 104 CFU g-1 silage in the control to 4.60 104 CFU g-1 in silage treated with FPA+i. The applied conservants increased (P«0.05) the content of DM from 255.00 g • kg 1 in control to 266.60 g • kg-1 with PA, WSC from 27.10 g • kg 'DM to 30.50 g • kg -1DM with FPA+i and ethanol from 9.10 g • kg 'DM to 11.21 g • kg -1DM with FPA+i in the silage. The additives decreased concentrations of lactic acid, acetic acid and crude protein concentration after opening the barrels. The diversifying factors decreased the pH value in the examined silage. The experimental conservants were found to improve the aerobic stability of silages after 7 days of air exposure.
The objective of this study was to determine the influence of biological silage additive (Bonsilage) on the hygiene quality and nutritive value of maize and grass-legume silages. The experiments were conducted on FAO 240 maize (Zea mays L.) and a mixture of italian ryegrass (Lolium multiflorum L.), 50% with alfalfa (Medicago media Pers.), 50%. Group 1 was a control and comprised silage without any additives, group 2 was ensiled with the addition of 4 cm3 kg-1 biological silage additive. After 60 days of silage process individual silages were subjected to microbiological composition, and chemical analyses of silages were also determined. Similar analyses were repeated at day 7 following exposure to oxygen. The applied biological silage additive was found to reduce (P<0.05) numbers of Clostridium, Enterobacteriaceae, yeasts and mold fungi cells, and increase (P<0.05) the number of LAB (lactic acid bacteria) in comparison with the control in both silages. Chemical analysis of the maize silage showed that the biological additive caused an increase (P<0.05) in DM (dry matter), CP (crude protein), WSC (water soluble carbohydrates), LA (lactic acid), AA (acetic acid), ethanol, and a decrease (P<0.05) in the concentration of BA (butyric acid), N-NH3 and pH value in comparison with the control. Chemical analysis of silage samples from the grass-legume mixture showed that the additive caused an increase (P<0.05) in the content of DM, CP, WSC, LA and AA in comparison with the control. Samples of silage with the addition of an inoculant were characterized by a lower (P<0.05) content of BA, N-NH3, ethanol and pH value. The biological additive impoved the aerobic stability of silages in the aerobic phase.
This research was carried out to determine the effects of bacterial silage inoculant using as silage additives on the fermentation charakteristics, cell wall contents and aerobic stability of maize silages. Maize silage was harvested in the 89 (BBCH) developmental ripening stage. Biological additive was used as additive which contains Enterococcus faecium PCM 1858, Pediococcus acidilactici PAL-34, Lactobacillus plantarum PCM 493, Lactobacillus buchneri DSMZ 5987, Lactobacillus rhamnosus PCM 489, Lactobacillus brevis PCM 488, Lactobacillus lactis PCM 2379. Maize was ensiled in 4 dm3 special PCV laboratory microsilos with a cover permitting gaseous products. The microsilos were stored at 10–15±2şC undr laboratory conditions. Microsilos from each group were sampled for microbiological and chemical analyses on the days 3, 14, 21 and 60 after ensiling, whereas aerobic stability was determined after 7 days. As a result, bacterial silage inoculant improved fermentation, decreased cell wall contents, deoxynivalenol (DON) concenration, do not improved aerobic stability of maize silage after 7 days exposure to air.
Maize attacked by the facultative biotrophic smut pathogen, Ustilago maydis (Basidomycetes) was ensiled in microsilos in two combinations of infected and non-infected plants, with or without biological (Polmasil) or chemical (Kemisile 2000) additives. The silage was subjected to chemical and microbiological analyses. The aerobic stability of the silage was tested. Ustilago maydis constituted up to 6.22% of the total yeast content (CFU g-1) in the infected and 0.22% in the noninfected material. Silage made from infected plants had a higher content of fungi (6.45 vs 4.54), moulds (6.20 vs 4.54), and yeast (6.08 vs 3.71) expressed as log10 CFU g-1 as compared with noninfected plants. The use of the chemical additive decreased these effects. The contents of ochratoxin, zearalenone, and deoxynivalenol were low and did not change during the ensiling. The cytotoxicity test did reveal toxicity of silage from infected plants, however. It seems that the observed toxicity is the effect of toxins other than those assayed.
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