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In the year 2000 the occurrence of Azotobacter spp. in 31 Polish soils was examined and results of this study were compared with those published by Ziemięcka for 28 soils analysed in 1917-18. Almost 52% of the soils tested in 2000 were colonised by Azotobacter spp. indicating that intensification of agricultural practices that took place during the past century has not significantly changed (50% soils with Azotobacter examined in 1917-18) the colonisation of Polish soils by the studied bacteria. When present, numbers of these bacteria varied widely, from several (soils No. 17 and 31) to almost 10,000 cfu g⁻¹ of the soil No. 25.
The aim of the study was to optimise the parameters (temperature and pH) of potato slops biodegradation with a mixed population of thermophilic and mesophilic aerobic bacteria of the genus Bacillus. Temperature and pH were optimised during 93-hour growth in the shake-flasks at 30 to 60°C and pH 3.88 to 9.0. The course of the biodégradation process at optimal temperature and pH, and with increased oxygen supply, was investigated in an aerated stirred-tank bioreactor. During cultivation in shake-flasks, the following phenomenon was observed: the higher was the process temperature, the sooner the maximal number of cells was achieved. Taking into account the need of maximising the extent of the COD reduction, the optimal temperature and pH values (calculated on the basis of experimental data) were 44.6°C and 7.40. With such parameters, a 22.5% reduction in COD was obtained. The highest number of cells (2.3x109/mL) was achieved at 30°C and pH 5.96. The biodégradation of potato slops in the stirred-tank bioreactor yielded an 86.5% reduction in COD. The process was carried out for 53 hours but most of the pollutants were degraded at the initial stage (21 h). Of the compounds which were utilised as carbon sources and occurred in the highest amounts, lactic, propionic and acetic acids were removed completely (100%), while the removal of reducing substances and glycerol exceeded 90%.
The aim of this study was to determine the in vitro resistance of aerobic bacteria isolated from the uterine pathological secretion of 312 dairy cows with clinical metritis and clinical endometritis to antibiotics. Animals with pathological discharges from the vagina observable between the 7th and 50th day after parturition were diagnosed clinically per vaginam and per rectum and then swabs from uteri lumen were aseptically collected. Bacteriological examinations of swabs were performed according to commonly accepted rules. Sensitivity to antibiotics was tested by the disk diffusion method and performed according to CLSI (formerly NCCLS) guidelines in Mueller-Hinton agar. The bacteria isolated were mostly Arcanobacterium pyogenes, Escherichia coli, non E. coli Gram-negative rods, Streptococcus species and Staphylococcus species. Strains of Arc. pyogenes were the most susceptible to amoxicillin/clavulanic acid (97.3%), ceftiofur (98%) and bacitracine (96.7%). E. coli isolates were the most susceptible to norfloxacin (100%), marbofloxacin (100%), rifaximin (97%), gentamycin (96%) and amoxicillin/clavulanic acid (95.5%). Other Gram-negative bacteria were the most sensitive to norfloxacin (100%), neomycin (100%) and cefoperazon (95%). Streptococcus species were the most susceptible to amoxicillin/clavulanic acid (94.6%), ampicillin (92.3%), norfloxacin (92%), cephapirine (88%), cefoperazone (86.5%), rifaximine (85.7%) and penicillin (84.9%). The highest in vitro activity against Staphylococcus spp. was demonstrated by amoxicillin/clavulanic acid (100%), norfloxacin (100%), neomycin (93.6%) and cefoperazone (85.7%). Arc. pyogenes were the most resistant to oxytetracycline and cloxacillin, E. coli to ampicillin and cephapirin, non-E. coli Gram-negative rods to ampicillin and cephapirin, Streptococcus spp. to neomycin and oxytetracycline, and Staphylococcus spp. to ampicillin.
The aim of the study was to determine the influence of multicomponent preser­vative in a loose form on the reduction of bacteria, fungi and mycotoxins in mixtures for pregnant and lactating sows. The mixtures were stored for 3 months. The research material were 6 mixtures for pregnant sows and 6 mixtures for lactating ones, differing with the level of total protein, the addition of crystalline amino acids and preservative. The mixtures were produced from the same components according to the receipt established. The preserva­tive in amount of 0.8% was introduced to the experimental mixtures. The following acids were the active components of the preservative: formic, propionic, phosphoric, citric and benzoic. The control mixtures and those with the preservative were stored for 3 months in a storehouse. After 1st and 3rd month of the storage, the total number of bacteria and fungi was determined, and after 3 months of the storage the content of alfatoxin B,, ochratoxin A, zearalenone and deoxynivalenol was determined. The preservative applied influenced the reduction in the number of aerobic mesophilic bac­teria from 1.3xl0ń to 3.1 x I02 and a decrease in fungi number from 3.9x10' to 4.0x10' cfu/g. The highest bacteria reduction was noted in the case of mixtures with the lowest protein level with an addition of crystalline amino acids. The preservative reduced the content of alfatoxin B(, from 2.0 to 1.0 ppb. The preservative assessed did not influence the reduction of ochratoxin A, zearalenone and deoxynivalenol in a significant manner. The content of these mycotoxins was subject to a decrease of only 20 to 30%.
Celem pracy była ocena jakości sensorycznej, mikrobiologicznej i cech fizykochemicznych kolendry mielonej, poddanej dekontaminacji z zastosowaniem pary wodnej oraz określenie jej trwałości podczas przechowywania w ciągu 12 miesięcy. W próbach kolendry, zarówno przed, jak i po dekontaminacji oznaczano zawartość wody, olejków eterycznych, popiołu ogólnego i nierozpuszczalnego, liczbę bakterii tlenowych, grzybów pleśniowych, pałeczek z grupy coli oraz obecność Salmonella. Stwierdzono, że jakość sensoryczna kolendry po dekontaminacji uległa niewielkiemu pogorszeniu. Wszystkie cechy fizykochemiczne po procesie dekontaminacji były zgodne z wymaganiami określonymi w polskiej normie przedmiotowej, nawet zawartość olejków eterycznych, pomimo tego, że w oczyszczonej kolendrze mielonej było ich mniej o ok. 40% w stosunku do surowca wyjściowego. Dekontaminacja skutecznie zmniejszała zanieczyszczenie mikrobiologiczne, jedynie w 4 próbach kolendry mielonej liczba bakterii tlenowych przekraczała nieznacznie dopuszczalny poziom. Wraz z upływem czasu przechowywania zapach i smak kolendry były mniej intensywne, podczas gdy barwa jej pozostawała bez zmian. Po 12 miesiącach składowania straty olejków w kolendrze wynosiły średnio, odpowiednio w próbach przed i po dekontaminacji, 40 i 25%; zanieczyszczenie bakteriami tlenowymi i pałeczkami z grupy coli było podobne, jak na początku okresu przechowywania, natomiast liczba grzybów uległa niewielkim zmianom.
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