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1

Apoptotic index and histological assessment of renal tubular epithelial cells during anthracycline-induced apoptosis. Influence of time

100%
Pedrycz A., Boratynski Z., Drelich G.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 1
55-58
The presented study describes renal tubular epithelial cells morphology in rats during adriamycin therapy. It was demonstrated that one dose of the compound can induce increasing-with time-apoptosis in the cells. A statistically-significant increase in the number of apoptotic cells was observed in experimental groups compared to controls. The highest percentage of apoptotic cells was found in rats 7 weeks after adriamycin administration. The number of apoptotic cells in these animals was statistically significantly higher than that in the rats 4 weeks after adriamycin administration.
2

Effect of H2O2 on adriamycin-induced cytotoxicity in vitro

100%
Anuszewska E. L.
Archivum Immunologiae et Therapiae Experimentalis
|
1994
|
tom 42
|
nr 4
3

Ultrastructural and immunohistochemical evaluation of apoptosis in foetal rat liver after adriamycin administration

100%
Pedrycz A., Boratynski Z., Wieczorski M., Visconti J.
Bulletin of the Veterinary Institute in Puławy
|
2005
|
tom 49
|
nr 4
4

Capability of adriamycin and busulfan to induce adaptive response in vitro

100%
Anuszewska E. L., Koziorowska J. H.
Archivum Immunologiae et Therapiae Experimentalis
|
1999
|
tom 47
|
nr 1
5

A transferrin conjugate of adriamycin-synthesis and potential chemotherapeutic efficacy

100%
Lubgan D., Jozwiak Z.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
6
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Content available

The influence of adriamycin on the CD4 and CD8 cells in the course of trichinellosis in mice

100%
Karmanska K., Houszka M., Mista D., Stefaniak E.
Wiadomości Parazytologiczne
|
1994
|
tom 40
|
nr 4
The effect of adriamycin on the behaviour of CD4⁺ and CD8⁺ cells in the course of trichinellosis in mice has been studied. The animals infected with 200 larvae per mouse were administered intraperitoneally adriamycin (Adriblastin from Farmitalia) at 1st and 28th day post infection (dpi), in a dosis of 0.2 mg. The mice were killed weakly for 6 weeks and then at the 60th dpi. The examinations were made on histologic sections from the spleen, mesenteric lymph node, jejunum and masseter muscle using immunofluorescent and immunoenzymatic methods with monoclonal antibody. The mice receiving adriamycin exhibited more CD8⁺ cells in the intestinal mucosa and by the end of the experiment also in the muscles in comparison with the control animals, which, however, did not affect the course of the infection.
7

The effect of new anthracycline derivatives on the induction of apoptotic processes in human neoplastic cells

100%
Gruber B. M., Anuszewska E. L., Priebe W.
Folia Histochemica et Cytobiologica
|
2004
|
tom 42
|
nr 2
8

DNA damage and repair in normal and neoplastic cells treated with adriamycin

100%
Anuszewska E. L., Gruber B.
Acta Biochimica Polonica
|
1994
|
tom 41
|
nr 4
Adriamycin (ADR), a common antineoplastic drug, was used to study DNA repair synthesis, cell cytotoxicity and DNA single strand breaks in normal human fibroblasts — CLV98 and human melanoma cells — ME18. No repair synthesis was observed in ME18 and CLV98 cells exposed to adriamycin in concentrations up to 10~5 M. ME18 cells were less sensitive to ADR treatment than CLV98 cells. Adriamycin-induced DNA single strand breaks (at ADR concentration: 1 pg/ml) were incompletely repaired in ME18 cells and unrepaired in CLV98 cells within 24 h after drug removal. Within 48 h strand breaks were completely repaired in both kinds of cells. No repair of single strand breaks was observed in ME18 and CLV98 cells after drug treatment in the concentration of 5 Jig/ml.
9

Immunolocalization in portal acinus of the liver of different types of adriamycin induced cell death depending on time

84%
Pedrycz A., Boratynski Z., Mendocha J.
Medycyna Weterynaryjna
|
2014
|
tom 70
|
nr 02
The ability to control the phenomenon of apoptosis, its induction or inhibition, raises hopes for treating many diseases including cancer. Adriamycin, an antibiotic that is wildly used after treating cancer, induces apoptosis in liver cells in a certain and relatively quick way after its application. The aim of the work was to obtain and examine the model of apoptosis and necrosis of hepatocytes with respect to their response to different damaging stimuli (adriamycin) depending on the time after the application in correlation with the ultrastructural construction, which is the result of the different location of hepatocytes within the portal acinus (of Rappaport). There were 32 female white Wistar rats used in the study. They were divided into 4 groups (2 experimental and 2 control), 8 animals in each group. The adriamycin dose of 5 mg/kg was administered intraperitoneally to the rats in groups I and II and then the rats were decapitated after 4 weeks (group I) and after 8 weeks (group II). The rats in the control groups were given 0.5 ml 0.9% NaCl solution and then decapitated after 4 weeks (group III) and 8 weeks (group IV). In the research, preparations made from fragments of the right liver lobe were used for histological observations and immunohistochemical studies. In the immunohistochemical studies, a three-stage method was used. According to this method, hepatocytes were examined qualitatively and quantitatively for the presence of proteins involved in apoptosis, to which the death signals run: through mitochondrial pathways (caspase 3 and caspase 9), through intrinsic pathways by endoplasmic reticulum (caspase 3 and caspase 12), through extrincic pathways (caspase 3 and caspase 8) and one from inflammatory markers: caspase 1. Histological images showed that the apoptosis phenomenon occurs after the administration of adriamycin in hepatocytes in a zonate way and is dependent on the time that has elapsed since its administration. Immunohistochemical studies showed, in both a qualitative and quantitative way, a phenomenon of apoptosis in hepatocytes (executive caspase 3) and necrosis (caspase 1). It was also proved that the signal for the induction of apoptosis showed zonation and mainly followed the mitochondrial pathway (caspase 9); the intrinsic pathway by endoplasmic reticulum was much less common (caspase 12); while even more rarely caspase 8 was identified as a marker of an extrinsic pathway to induce apoptosis.
10

Heat shock protein 70 as a marker of oxygen stress in kidneys of rat offspring. Immunohistochemical assessment

84%
Pedrycz A., Boratynski Z., Visconti J.
Bulletin of the Veterinary Institute in Puławy
|
2006
|
tom 50
|
nr 2
11

Apoptosis of foetal renal tubular epithelial cells as late effect of adriamycin action. Immunohistochemical assessment of caspase 3 expression

84%
Pedrycz A., Boratynski Z., Wieczorski M., Czerny K.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 1
In previous papers, we noticed that adriamycin given to female rats before their planned pregnancy has a delaying effect under the form of apoptosis for foetal hepatocytes. The purpose of present study was for a quantitative assessment of foetal renal tubular epithelial cells, as an effect means of delaying adriamycin action (apoptotic index). Expression of effector caspase 3 was also assessed. In the investigations, a standard three-step immunohistochemical method was used. The area covered by positive caspase 3 reaction was examined. In the kidneys of foetuses from the experimental group, we noticed an increase in the apoptotic index; furthermore, immunohistochemical reaction for caspase 3 covered a statistically significantly larger range as compared to the control group. The delayed effect of adriamycin, which was given to female rats before pregnancy, was an increase in apoptosis in foetal renal tubular epithelial cells.
12

Metabolic activation of adriamycin by NADPH-cytochrome P450 reductase; overview of its biological and biochemical effects

84%
Bartoszek A.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 2
NADPH-cytochrome P450 reductase (P450 reductase) is one of the enzymes impli­cated in the metabolism of adriamycin, a very important clinically used antitumour drug. However, apart from the enzyme involvement, so far little was known about the chemical route and biochemical effects of this process. We demonstrated that the ap­plication of P450 reductase simultaneously with adriamycin to tumour cells in culture significantly increased cytotoxicity of the drug. Under tissue culture conditions, we noticed also that, in the presence of P450 reductase, adriamycin metabolite(s), dis­playing an altered spectrum within the visible light range were formed. This observa­tion was taken adavantage of to study the metabolism of adriamycin in cell-free sys­tems, using initially the enzyme isolated from rat liver and the recently obtained re­combinant human P450 reductase. The reductive conversion of the drug turned out to be a multi-stage process, which occurred only under aerobic conditions and was ac­companied by excessive NADPH consumption. Further research carried out with the aid of radical scavengers and radiolabelled adriamycin revealed that the enhance­ment of biological activity of adriamycin by P450 reductase stemmed from the forma­tion of alkylating metabolite(s) rather than from the promotion of redox cycling known to be induced in the presence of anthracyclines.
13

Effect of adriamycin and gamma-radiation on cultured mammalian cells measured by flow cytometry

84%
Koceva-Chyla A., Distel L., Zaborowski A., Skierski J., Rozga B., Schussler H., Jozwiak Z.
Current Topics in Biophysics
|
1994
|
tom 18
|
nr 2
14
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Content available

L-arginine decreases heat shock protein 70 [marker of environmental stress] expression in kidney cells of rat fetuses during apoptosis - late effect of adriamycin action

84%
Pedrycz A., Brzeski Z.
Annals of Agricultural and Environmental Medicine
|
2006
|
tom 13
|
nr 1
Both Adriamycin and nitric oxide (NO) cause apoptosis acting through or as free radicals inciting oxidative stress in the cell. However, in some tissues the antiapoptotic action of NO was described, thereby the impact of NO on cell apoptosis is not finally recognized. In this study, a trial of the evaluation of exogenous NO (L-arginine) impact on apoptosis induced by Adriamycin in fetal kidney cells was undertaken. For this reason, the expression of Heat Shock Protein 70 (HSP 70), environmental stress marker, as a sensitive biomarker of oxidative stress induced in fetal kidney cells with Adriamycin given to mothers prior to pregnancy was studied using immunohistochemical method. The expression of HSP 70 in fetal kidney cells, whose mothers received apart from Adriamycin, L-arginine (as NO substrate) was also evaluated. The results of the study pointed to the fact that the exogenous NO (L-arginine) could be helpful in inhibition of intensified apoptosis in fetal cells as a late effect of Adriamycin action.
15

DNA repair of adriamycin induced damage in mouse and human cells differing in catalase activity

84%
Gruber B. M., Anuszewska E. L.
Acta Biochimica Polonica
|
1998
|
tom 45
|
nr 2
Catalase is known to counteract cytotoxic effect of adriamycin, used as an anti-neoplastic drug. In cells with low catalase activity no repair of adriamycin induced lesions was observed up to 48 h post treatment. In cells with high catalase activity after 48 h the repair was either complete or partial depending on the human or mouse cell type used.
16

P-53 protein and proteins from BCL-2 family in the heart of rat after adriamycin administration. Immunohistochemical evaluation

67%
Boratynski Z., Pedrycz A.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
|
2006
|
tom 09
|
nr 2
Adriamycin (ADR) – the antineoplastic antibiotics has confirmed proapoptotic activity, mainly on neoplastic cells and young quick dividing cells. Cardiotoxicity of Adriamycin is limitating in antineoplastic therapy. The purpose of study was an evaluation of internal pathway of induction of signal to the apoptosis in myocardial cells of rat, which had administered Adriamycin. The sign of late cardiotoxicity after Adriamycin is coagulative necrosis. In present study was noticed also increased apoptosis of cells in rat heart, which was induced via mitochondrial pathway, with activation of p-53 protein and with BAX/Bcl-2 ratio > 1 – with prevalence of proapoptotic BAX protein.
17

Ultrastructural evaluation of renal convoluted tubules of female rats, which had pregnancy complicated with secondary preeclampsia

67%
Pedrycz A., Wieczorski M., Boratynski Z., Czerny K., Flieger S.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
|
2005
|
tom 08
|
nr 2
18

The ability of new formamidine sugar-modified derivatives of daunorubicin to stimulate free radical formation in three enzymatic systems: NADH dehydrogenase, NADPH cytochrome p450 reductase and xanthine oxidase

67%
Pawlowska J., Tarasiuk J., Borowski E., Wasowska M., Oszczapowicz I., Wolf C. R.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 1
Some sterically hindered N-substituted derivatives of daunorubicin are known to be poor substrates for NADH dehydrogenase, NADPH cytochrome P450 reductase and xanthine oxidase. In consequence, poor oxygen radical generation by these compounds is observed. In this study we examined a new family of sugar-N-substituted derivatives of daunorubicin bearing a bulky substituent introduced on the nitrogen atom through the amidine spacer. These compounds were found to be very active in radical formation catalyzed by all three studied enzymes. Thus, the introduction of a heterocyclic ring, even if it is bulky but flexible, on the nitrogen atom of daunosamine moiety through the one-atom spacer (amidine group), does not induce the steric hindrance effect on the interaction of daunorubicin derivatives with these flavoprotein enzymes.
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