Wyniki wyszukiwania

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Changes in the content of lipids and the composition of fatty acids in leaves of non-vernalized winter rape (Brassica napus L. var. oleifera) grafted on vernalized stock

100%

Filek M., Dubert F., Filipek W., Pienkowski S., Augustyniak G.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1988

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tom 36

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nr 7-9

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Motility parameters and intracellular ATP content of rabbit spermatozoa stored for 3 days at 15 degrees Celsius

84%

Gogol P.

Folia Biologica

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2013

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tom 61

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nr 1-2

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Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa

84%

Kordan W., Lecewicz M., Strzezek R., Dziekonska A., Fraser L.

Polish Journal of Veterinary Sciences

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2010

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tom 13

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nr 4

The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treated with different concentrations of exogenous PAF (1 × 10⁻³M, 1 × 10⁻⁴M, 1 × 10⁻⁵M and 1 × 10-6M) and examined at different time intervals (0, 30, 60 and 120 min). Cryopreserved semen treated without PAF was used as the control. Sperm quality was evaluated for motility (computer-assisted semen analysis, CASA), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (SYBR-14/PI assay and Hoechst 33258). Also, ATP content of spermatozoa was determined using a bioluminescence assay. Treatment of cryopreserved semen with 1 × 10⁻³ M PAF at 120 min of incubation resulted in significantly higher total sperm motility compared with the control. It was observed that PAF-improved total sperm motility was concurrent with enhanced sperm motility patterns after treatment of cryopreserved semen. Treatment of cryopreserved semen with PAF did not improve either sperm mitochondrial function or plasma membrane integrity, as monitored by different fluorescent membrane markers. Furthermore, ATP content of cryopreserved spermatozoa was significantly higher when PAF was used at a concentration of 1 × 10⁻³ M compared with the control and other PAF treatments, regardless of the incubation time. The findings of this study indicated that treatment with 1 × 10⁻³ M PAF at 120 min of incubation rendered better quality of cryopreserved canine semen, which was associated with improved sperm motility parameters and ATP content. It can be suggested that exogenous PAF addition is beneficial as a supplement for canine semen extender used for cryopreservation.

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Changes of ATP content in leaves of non-vernalized grafts of winter rape (Brassica napus L. var. oleifera) after grafting onto vernalized stock

84%

Dubert F., Filek W., Pienkowski S., Augustyniak G., Filek M.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1988

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tom 36

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nr 7-9

Ograniczanie wyników

2 Bulletin of the Polish Academy of Sciences. Biological Sciences

1 Folia Biologica

1 Polish Journal of Veterinary Sciences

2 Augustyniak G.

2 Dubert F.

2 Filek M.

2 Pienkowski S.

1 Dziekonska A.

1 Filek W.

1 Filipek W.

1 Fraser L.

1 Gogol P.

1 Kordan W.

1 Lecewicz M.

1 Strzezek R.

1 2013

1 2010

2 1988

Changes in the content of lipids and the composition of fatty acids in leaves of non-vernalized winter rape (Brassica napus L. var. oleifera) grafted on vernalized stock

Motility parameters and intracellular ATP content of rabbit spermatozoa stored for 3 days at 15 degrees Celsius

Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa

Changes of ATP content in leaves of non-vernalized grafts of winter rape (Brassica napus L. var. oleifera) after grafting onto vernalized stock