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1

Cross-resistance to five glucocorticoids in childhood acute lymphoblastic and non-lymphoblastic leukemia samples tested by the MTT assay: Preliminary report

100%
Styczynski J., Wysocki M., Debski R., Balwierz W., Rokicka-Milewska R., Matysiak M., Balcerska A., Kowalczyk J., Wachowiak J., Sonta-Jakimczyk D., Chybicka A.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 1
In vitro antileukemic activity of five glucocorticoids and their cross-resistance pat­tern in childhood acute lymphoblastic and non-lymphoblastic leukemia were deter­mined by means of the MTT assay in 25 leukemia cell samples of childhood acute leukemias. The equivalent antileukemic concentrations of the drugs tested were: 34 uM hydrocortisone (HC), 8 uM prednisolone (PRE), 1.5 uM methylprednisolone (MPR), 0.44 uM dexamethasone (DX) and 0.22 Mbetamethasone (BET). In comparison with initial ALL cell samples, the relapsed ALL group was more resistant to PRE (38-fold, p = 0.044), DX (> 34-fold, p = 0.04), MPR (38-fold), BET (45-fold) and HC (33-fold). TheAMLcell samples were even more resistant to: PRE (>85-fold, p=0.001), DX (> 34-fold, p = 0.004),MPR(> 69-fold, p = 0.036), BET (> 69-fold, p = 0.038) and HC (54-fold, p = 0.059) when compared with ALL on initial diagnosis. A significant cross-resistance among all the glucocorticoids used was found. Only in some individual cases the cross-resistance was less pronounced.
2

Platelet-activating factor changes in phospholipid extracts from the plasma, peripheral blood mononuclear cells and bone marrow mononuclear cells of patients with acute leukemia - A 31P MRS in vitro study

84%
Kuliszkiewicz-Janus M., Tuz M. A., Kielbinski M., Baczynski S., Jazwiec B., Sladowska H.
Cellular and Molecular Biology Letters
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2008
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tom 13
|
nr 1
58-66
The aim of this investigation was to evaluate the changes in PAF concentrations in the plasma, PBMC and BMMC of patients with acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML). The plasma was from 23 healthy volunteers (HV) and 44 patients with AL (16 ALL, 28 AML). The PBMC were from 15 HV and 55 patients with AL (18 ALL, 37 AML), and the BMMC from 40 patients with AL (11 ALL, 29 AML). Methanol-chloroform phospholipid extraction from 60 × 106 cells (PBMC or BMMC) was performed according to a modified version of Folch’s method. 31P MRS data was obtained on an AMX 300 Bruker spectrometer (7.05 T). The PAF concentration in the plasma of the patients with ALL or AML was lower than that for the healthy volunteers. The PAF concentration in the plasma of the patients with ALL did not differ significantly from that of the patients with AML. In the case of both the PBMC and BMMC, the PAF concentration was significantly diminished in patients with ALL relative to the concentration for those with AML and for the healthy volunteers. No differences were observed in the PAF concentrations for the AML patients and the healthy volunteers.
3

Apoptosis monitoring in human acute leukaemia cells stimulated by Bcl-2 antisense oligonucleotide and BAY K 8644

84%
Kocki J., Kocki T., Lancut M., Bogucka-Kocka A., Cioch M., Dmoszynska A., Wojcierowski J.
Polish Journal of Environmental Studies
|
2004
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tom 13
|
nr Suppl.2, Part 1
In this study we investigated effects of bcl-2 antisense oligonucleotides (AS) and BAY K 8644 (B), calcium channel agonist, on human leukaemic cells from non-treatment patients with acute myeloblastic leukaemias. Apoptotic cells were observed in electron and fluorescent microscopes. When we examined the effects of AS + B on cells, level of bcl-2 mRNA decreased more as in cells stimulated by AS only. These observations indicate that AS with B in human leukaemic cells disturb cell viability by inducing apoptosis.
4

P MRS analysis of the phospholipid composition of the peripheral blood mononuclear cells [PMBC] and bone marrow mononuclear cells [BMMC] of patients with acute leukemia [AL]

84%
Kuliszkiewicz-Janus M., Tuz M. A., Kielbinski M., Jazwiec B., Niedoba J., Baczynski S.
Cellular and Molecular Biology Letters
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2009
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tom 14
|
nr 1
35-45
The aim of this study was to evaluate the phospholipid concentration in acute leukemia (AL) blast cells from peripheral blood (PBMC) and bone marrow (BMMC). In vitro 31P Nuclear Magnetic Resonance Spectroscopy (31P MRS) was used. The integral intensities of the resonant peaks and the phospholipid concentrations in PBMC and BMMC were analyzed. Differences in the phospholipid concentrations in cells from myeloblastic or lymphoblastic lines were also evaluated. This investigation was carried out on phospholipid extracts from PBMC and BMMC from 15 healthy volunteers and 77 patients with AL (samples taken at the moment of diagnosis). A significant decrease in sphingomyelin (SM) and phosphtidylserine (PS) was observed in the PBMC of patients with AL relative to the results for the healthy volunteers. For ALL, we found a significant decrease in the concentration of phosphatidylcholine plasmalogen (CPLAS), SM, PI+PE (phosphatidylinositol + phosphatidylethanolamine) and PS in comparison with the results for healthy volunteers and patients with AML. Experiments with BMMC cells revealed a significant decrease in the concentration of CPLAS, SM, PI+PE, and PS in ALL relative to AML. Additionally, a significant decrease in phosphatidylcholine (PC) concentration was observed in ALL compared to AML. If the phospholipid extracts were taken simultaneously from the same patient, there were no significant differences in the integral intensities and phospholipid concentrations between PBMC and BMMC.
5

Cytogenetic analysis and clinical significance of chromosome 7 aberrations in acute leukaemia

84%
Brozek I., Babinska M., Kardas I., Wozniak A., Balcerska A., Hellmann A., Limon J.
Journal of Applied Genetics
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2003
|
tom 44
|
nr 3
6

Expression of uPAR [urokinase plasminogen activator receptor], P-glycoprotein 170 and VCAM-1 [vascular cell adhesion molecule] on acute leukaemia cells

84%
Cioch M., Hus I., Jawniak D., Malek M., Manko J., Goracy A., Dmoszynska A.
Bulletin of the Veterinary Institute in Puławy. Supplement
|
2002
7

Lipid carriers for genetic drugs in gene therapy of acute leukemias

84%
Wyrozumska P., Stebelska K., Sikorski A. F.
Cellular and Molecular Biology Letters
|
2005
|
tom 10
|
nr Suppl.
8

The activity of human telomerase in the cells of acute leukaemias

84%
Kocki J., Kolano J., Cioch M., Dmoszynska A., Wojcierowski J.
Folia Morphologica
|
2004
|
tom 63
|
nr 1
Telomeres are the end fragments of chromosomes formed by a number of non-coding double-stranded TTAGGG repeats in vertebrates. During cell division the number of repeats decreases, leading to cell senescence or apoptosis. In immortal cells, including cancer cells, the telomere length is stable and maintained by, among other factors, telomerase. The aim of the study is to compare telomerase activity in normal lymphocytes and in leukaemic cells. Samples of acute leukaemia cells, HL 60 cell line and the lymphocytes of healthy volunteers were examined. Telomerase analysis was performed using TeloTAGGG Telomerase PCR ELISAplus (Roche). The relative telomerase activities (RTA) in leukaemic and normal cells were analysed. A high level of RTA was observed in leukaemic cells.
9

Lipid changes occuring in the course of hematological cancers

67%
Kuliszkiewicz-Janus M., Malecki R., Mohamed A. S.
Cellular and Molecular Biology Letters
|
2008
|
tom 13
|
nr 3
465-474
The relationship between plasma lipid levels and mortality from cardiovascular diseases has been shown in many studies, but there has been far less investigation into their relationship to non-cardiovascular diseases. The aim of this study was to investigate the lipid profile of individuals with hematological malignancies and its relationship to disease activity. 238 patients were included in the study: 84 with acute leukemia, 62 with non-Hodgkin lymphoma, 35 with Hodgkin’s lymphoma, 32 with multiple myeloma, and 25 with myeloproliferative syndrome. The HDL cholesterol level of the patients differed to that of the individuals in the control group in the active disease period for all the analyzed disorders, but only remained statistically significant in the acute leukemia and non-Hodgkin lymphoma groups during the remission period. Smaller differences were observed for the remaining lipid fractions, except for the triglyceride level, which increased in the active disease period in all the analyzed disorders except non-Hodgkin lymphoma. The most pronounced changes in the lipid fractions occurred in the HDL cholesterol level, and were the most remarkable for acute leukemia.
10

The influence of intracellular idarubicin and daunorubicin levels on drug cytotoxicity in childhood acute leukemia

67%
Styczynski J., Wysocki M., Debski R., Kurylak A., Balwierz W., Rokicka-Milewska R., Matysiak M., Balcerska A., Kowalczyk J., Wachowiak J., Sonta-Jakimczyk D., Chybicka A.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 1
Uptake and efflux of two anthracyclines, idarubicin (IDA) and daunorubicin (DNR), was studied in childhood acute leukemia samples. A comparison of IDA and DNR transport phenomena in relation to drug cytotoxicity and expression of P-glycoprotein (PGP) was made. Intracellular content of IDA/DNR was determined by flow cytometry using the fluorescent properties of the drugs. In vitro drug cytotoxicity was measured by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. PGP expression was analysed by flow cytometry. The uptake and efflux rates were non-significantly higher for IDA than DNR. There were no differences between three types of leukemia with respect to drug content during accumulation and retention. After correction for the cell volume, intracellular concentration of both drugs in each moment of uptake and efflux was significantly lower in relapsed ALL and AML samples in comparison with initial ALL cells. Efflux, but not uptake, of both drugs was inversely correlated with PGP expression and IDA, but not DNR, cytotoxicity. The cytotoxicity was correlated with drug accumulation for both drugs and with drug retention for IDA. In conclusion, it seems that (1) intracellular content was related to the lipophilic properties of the drugs rather than to the type of leukemia, (2) decreased intracellular concentration of both drugs might have an impact on compromised therapy results in AML and relapsed ALL children, (3) IDA presents higher cytotoxicity, which possibly might be decreased by the presence of PGP. These results might have a practical impact on the rational design of new chemotherapy protocols.
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