Wyniki wyszukiwania

1

Effect of proflavine and its 2,7-dialkyl substituted derivatives upon initiation of transcription

100%

Piestrzeniewicz M. K., Szemraj J., Gniazdowski M.

Acta Biochimica Polonica

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1993

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tom 40

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nr 1

2

Uptake of acridinecarboxamide derivatives by L1210 cells

84%

Ciesielska E., Studzian K., Bazylak G., Pastwa E., Denny W. A., Szmigiero L.

Acta Biochimica Polonica

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1998

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tom 45

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nr 4

The uptake of six 9-aminoacridinecarboxamide derivatives by L1210 cells in relation to their lipophilicity and cytotoxic activity was studied. The amount of acridines taken up by cells was estimated by fluorimetric measurements. It was found that the uptake efficiency of this class of compounds by cells depends on the size of carboxamide residue as well as on position of the substituent. The increase of size of carboxamide chain resulted in the loss of capability of acridines to penetrate cell membrane. Cytotoxic effects of acridines were well correlated with the level of drugs accumulated by cells, whereas no clear correlation between uptake and lipophilicity was observed. It is concluded that uptake of 9-aminoacridinecarboxamides is the most important factor determining their antiproliferative activity.

3

Effect of DNA-interacting drugs on phage T7 RNA polymerase

84%

Piestrzeniewicz M., Studzian K., Wilmanska D., Plucienniczak G., Gniazdowski M.

Acta Biochimica Polonica

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1998

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tom 45

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nr 1

9-Aminoacridine carboxamide derivatives studied here form with DNA intercalative complexes which differ in the kinetics of dissociation. Inhibition of total RNA synthesis catalyzed by phage T7 and Escherichia coli DNA-dependent RNA polymerases correlates with the formation of slowly dissociating acridine-DNA complex of time constant of 0.4-2.3 s. Their effect on RNA synthesis is compared with other ligands which form with DNA stable complexes of different steric properties. T7 RNA polymerase is more sensitive to distamycin A and netropsin than the E. coli enzyme while less sensitive to actinomycin D. Actinomycin induces terminations in the transcript synthesized by T7 RNA polymerase. Despite low dissociation rates of DNA complexes with acridines and pyrrole antibiotics no drug dependent terminations are observed with these ligands.

Ograniczanie wyników

3 Acta Biochimica Polonica

2 Gniazdowski M.

2 Studzian K.

1 Bazylak G.

1 Ciesielska E.

1 Denny W. A.

1 Pastwa E.

1 Piestrzeniewicz M.

1 Piestrzeniewicz M. K.

1 Plucienniczak G.

1 Szemraj J.

1 Szmigiero L.

1 Wilmanska D.

2 1998

1 1993

Effect of proflavine and its 2,7-dialkyl substituted derivatives upon initiation of transcription

Uptake of acridinecarboxamide derivatives by L1210 cells

Effect of DNA-interacting drugs on phage T7 RNA polymerase