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Effect of fertilizer on soil reaction

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The aim of study was to determine the influence of growing fertilizer rates on soil reaction. Soil samples were taken from experimental plots, where different amounts of NPK fertilizers were applied for perennial grasses within 6 years (1986 - 1992). The experimental design was 12 treatments in 4 replications, and established in sod-gleysolic loamy sand. Potential acidification of soil as a result of fertilization was calculated based on CaCO₃ balance and obtained results validated with the analytical tests of topsoil (0 - 20 cm) and subsoil (20 - 40 cm) reaction before and after experiment. Obtained results showed low performance of data obtained by calculation CaCO₃ balance, thereby using only CaCO₃ balance, it is risky to forecast soil acidification. In spite ofthat the used fertilizer rates changed in the widely ranges between treatments (N₀P₀K₀ - N₃₀₀P₁₂₀K₂₄₀), fertilization had not significant influence on soil reaction.
In Poland 11% of soils feature elevated zinc content or slight zinc contamination. This investigation aimed at estimation of the effect of progressive acidification of slightly zinccontaminated soils on zinc content in plants and translocation of this metal downwards the soil profile. The study involved a two-year lysimetric experiment on lessive soil. The amount of zinc indicating slight soil contamination was introduced into 0.2 m of topsoil, which was subjected to progressive acidification with sulfuric acid solution in the course of the experiment. Zinc content proved to considerably increase in plants (barley straw and maize) only under strong acidification. Soil reaction did not significantly influence the zinc content in soil, both total and assayed in HCl zinc forms, while a considerable increase in easily soluble zinc forms (in CaCl2 solution) occurred on strongly acidified soils. Considering the whole research period, increasing soil acidification did not result in any alterations involving zinc content in Bbr and C horizons of soil profiles (below 30 cm).
The aim of the study was to investigate the effect of acidification and heat on the survival of Clostridium spores in third category animal by-products. Poultry minced meat and minced fish waste, contaminated with Clostridium sporogenes IW 1306 spore suspension, were subjected to the combined action of formic acid and high temperature of different values. To assess the efficiency of examined hygienization method, the spore number in biomass samples after acidification and heat treatment was estimated. For this purpose a 10-fold dilution series in peptone water was prepared and heat-treated at 80°C for 10 min. After cooling-down, one milliliter of each dilution was pour-plated onto DRCM medium solidified with agar. Statistical analyses were performed using the Statistica software. The results of the microbiological analysis show that lowering the pH to about 4 results in increasing the sensitivity of Clostridium sporogenes spores to a high – but not exceeding 100°C – temperature. The fastest inactivation of spores occurred after heating the acidified biomass at a temperature of 90°C. In the case of meat the 48-hour exposure to formic acid allowed the thermal inactivation of the spores within 15 minutes. The investigated method of hygienization may guarantee not only the inhibition of spores germination, but also enables their total elimination, reducing the epidemiological risk related to animal by-products handling.
The present study compares properties of podzolic soils developed from sandy materials overgrown by forests, affected either by alkalizing or acidifying emissions. The study entailed sampling 4 pedons in the vicinity of the Ożarów Cement Plant S.A. and 4 pedons near Rudniki village (within the impact zone of “Osiek” Sulphur Mine and Połaniec Power Plant). Soil samples were analyzed for basic chemicalproperties. The study showed significant changes in physicochemical properties of the alkalized podzolic soils: an increase in pH value, in base saturation of the exchange complex, contents of plant available potassium and magnesium, CaCO₃ content in organic and humic horizons and in salinity, and a decrease in organic carbon content and hydrolytic acidity, podzolic soils occurring in areas affected by anthropogenic acidification were characterized by decreased pH value, increased total sulphur and sulfate (S-SO₄) contents, increased hydrolytic and exchangeable acidity, and lowered base saturation of the exchange complex.
It has been suggested that Bax translocation to the mitochondria is related to apoptosis, and that cytosol acidification contributes to apoptosis events. However, the mechanisms remain obscure. We investigated the effect of acidification on Bax translocation and on ultraviolet (UV) light-induced apoptosis. The Bax translocation assay in vitro showed that Bax translocated to the mitochondria at pH 6.5, whereas no Bax translocation was observed at pH 7.4. VHDBB cells expressing the GFP-Bax fusion protein were treated for 12 h with a pH 6.5 DMEM medium, nigericin (5 μg/ml) and UV light (50 J/cm2), separately or in combination, and Bax translocation to the mitochondria was determined by SDS-PAGE and Western blot, and apoptotic cell death was detected by flow cytometry. The results showed that some of the Bax translocated to the mitochondria in the cells treated with the normal medium, nigericin and UV in combination, whereas all of the Bax translocated to the mitochondria in the cells treated with the pH 6.5 medium, nigericin and UV in combination. In VHDBB cells treated for 12 h with nigericin, UV alone, and UV and nigericin in combination, the respective rates of apoptotic cell death were 25.08%, 33.25% and 52.88%. In cells treated with pH 6.5 medium and nigericin, pH 6.5 medium and UV, and pH 6.5 medium, nigericin and UV in combination, the respective rates of apoptotic cell death increased to 37.19%, 41.42% and 89.44%. Our results indicated that acidification induces Bax translocation from the cytosol to the mitochondria, and promotes UV lightmediated apoptosis. This suggests that there is a possibility of improving cancer treatment by combining acidification with irradiation or chemotherapeutic drugs.
This study aimed to determine the influence of sewage sludge alkalization and acidification on the qualitative and quantitative composition of keratinolytic and keratinophilic fungi in a model experiment. The sludge was alkalized to pH 9 with 0.5N NaOH, 0.5N KOH, 0.5N NH₄OH or with 2% burnt lime mixed with powdered limestone (20g CaO + 5g CaCO₃), and acidified to pH 4 with 0.5N HCl, 0.5N HNO₃ or 0.5N H₂SO₄. The sludge with unmodified pH (6.5) served as control. The hair baiting method with four incubation temperatures (23, 29, 33, and 37°C) was used to examine fungi. The sludge alkalization with KOH, NaOH or CaO+CaCO₃ increased the number of keratinolytic fungi but decreased the number of keratinophilic fungi. The inhibition of the growth of keratinolytic fungi was observed due to the sludge acidification with H₂SO₄ or HCl. The sludge acidification with HNO₃ or HCl stimulated keratinophilic fungi to grow. Sludge acidification with HNO₃ eliminated keratinolytic fungi, while acidification with H₂SO₄ eliminated keratinophilic fungi. Results are discussed from ecological and epidemiological points of view.
In the course of the research (hydrobiological and hydrochemical) on the three mountain streams was found out that acidification was a main agent which disqualified a quality of their water. An attempt on neutralize of water of the Czerwień stream was undertaken by means of "cushions" (with chalk and without chalk) - an enclaves of the neutralized environment and with the use of model mechanism as well. The advantageous impact of the neutralized water on macrobenthos and fishes was found out and confirmed by means of numerous biological test.
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