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This report describes the historical development in the clinical application of chemical variables for the interpretation of acid-base disturbances. The pH concept was already introduced in 1909. Following World War II, disagreements concerning the definition of acids and bases occurred, and since then two strategies have been competing. Danish scientists in 1923 defined an acid as a substance able to give off a proton at a given pH, and a base as a substance that could bind a proton, whereas the North American Singer-Hasting school in 1948 defined acids as strong non-buffer anions and bases as non-buffer cations. As a consequence of this last definition, electrolyte disturbances were mixed up with real acid-base disorders and the variable, strong ion difference (SID), was introduced as a measure of non-respiratory acid-base disturbances. However, the SID concept is only an empirical approximation. In contrast, the Astrup/Siggaard-Andersen school of scientists, using computer strategies and the Acid-base Chart, has made diagnosis of acid-base disorders possible at a glance on the Chart, when the data are considered in context with the clinical development. Siggaard-Andersen introduced Base Excess (BE) or Standard Base Excess (SBE) in the extracellular fluid volume (ECF), extended to include the red cell volume (eECF), as a measure of metabolic acid-base disturbances and recently replaced it by the term Concentration of Titratable Hydrogen Ion (ctH). These two concepts (SBE and ctH) represent the same concentration difference, but with opposite signs. Three charts modified from the Siggaard-Andersen Acid-Base Chart are presented for use at low, medium and high altitudes of 2500 m, 3500 m, and 4000 m, respectively. In this context, the authors suggest the use of Titratable Hydrogen Ion concentration Difference (THID) in the extended extracellular fluid volume, finding it efficient and better than any other determination of the metabolic component in acid-base disturbances. The essential variable is the hydrogen ion.
Classically, the acid-base balance (ABB) is described by the Henderson-Hasselbach equation, where the blood pH is a result of a metabolic components – the HCO₃⁻ concentration and a respiratory component – pCO₂. The Stewart model assumes that the proper understanding of the organisms ABB is based on an analysis of: pCO₂, Strong Ion difference (SID) – the difference strong cation and anion concentrations in the blood serum, and the Acid total (Atot) – the total concentration of nonvolatile weak acids. Right sided heart failure in dogs causes serious haemodynamic disorders in the form of peripheral stasis leading to formation of transudates in body cavities, which in turn causes ABB respiratory and metabolic disorders. The study was aimed at analysing the ABB parameters with the use of the classic method and the Stewart model in dogs with the right sided heart failure and a comparison of both methods for the purpose of their diagnostic and therapeutic utility. The study was conducted on 10 dogs with diagnosed right sided heart failure. Arterial and venous blood was drawn from the animals. Analysis of pH, pCO₂ and HCO₃⁻ was performed from samples of arterial blood. Concentrations of Na+, K+, Cl-, Pinorganic, albumins and lactate were determined from venous blood samples and values of Strong Ion difference of Na+, K+ and Cl- (SID₃), Strong Ion difference of Na+, K+, Cl- and lactate (SID4), Atot, Strong Ion difference effective (SIDe) and Strong Ion Gap (SIG₄) were calculated. The conclusions are as follows: 1) diagnosis of ABB disorders on the basis of the Stewart model showed metabolic alkalosis in all dogs examined, 2) in cases of circulatory system diseases, methodology based on the Stewart model should be applied for ABB disorder diagnosis, 3) if a diagnosis of ABB disorders is necessary, determination of pH, pCO₂ and HCO₃⁻ as well as concentrations of albumins and Pinorganic should be determined on a routine basis, 4) for ABB disorder diagnosis, the classic model should be used only when the concentrations of albumins and Pinorganic are normal.
The comparative study was performed on 200 European brown hares. Group I consisted of 35 clinically healthy hares kept in cages. Group II comprised 165 clinically healthy hares caught alive in their natural environment. Following the premedication, arterial and venous blood was drawn from the hares of the two groups studied. In the arterial blood, the acid-base balance (ABB) parameters were determined - blood pH, pCO₂, t CO₂, HCO₃⁻, BB, and BE. In the venous blood, the activity of AST, ALT, and FA, concentration of urea, creatinine, total protein, albumins, globulins, cholesterol, triglycerides, glucose, WBC, RBC, Hb, and Ht were determined. In addition, concentrations of Ca²⁺, P, Mg²⁺, Na⁺, K⁺, and Cl⁻ were measured in the blood serum. It was demonstrated that only the contents of globulins and triglycerides were similar in groups I and II. Concentration of electrolytes and ABB parameters were close to each other in the two studied groups, except for concentration of Mg²⁺ and inorganic P, and CO₂ molecular pressure. In the light of the obtained results a question remains opened: whether successful breeding, understood as an increase in the number of offspring of the reintroduced individuals, is more likely to occur in the case of animals caught alive and, adapted to living in their natural environment, or in the case of caged animals.
Brachycephalic syndrome develops as a result of a specific build of splanchnocranium. In dogs of brachycephalic breeds, an impairment of correct gas exchange occurs. 5 French bulldogs at the age of 11-14 months, which suffered from continuous mixed-type dispnoea and lowered effort tolerance, were examined. Apart from the above-mentioned symptoms, the occurrence of strong external nostril stenosis was noted in the clinical examination. The symptoms observed together with the nostril stenosis indicated a possibility of an occurrence of the brachycephalic syndrome. 1 ml of full blood was drawn from the femoral artery. Acid-base balance parameters were determined in the arterial blood: pH, pCO₂, HCO₃ -, and pO₂. The wing-of-the-nostrils correction procedure were carried out in general anaesthesia. In premedication, the patients received medetomidine and after 15 minutes, fentanyl together with atropine. The induction of propofol was carried out. After four weeks from the procedure, blood was collected and the acid-base balance and pO₂ parameters were again determined. The obtained values of the acid-base balance and pO₂ parameters showed a noticeable influence of the wing-of-the-nostrils correction procedure on the values of the parameters determined. The results of the blood gasometry obtained prior to the procedure clearly indicate the occurrence of respiratory acidosis. The correction of wings of the nostrils significantly influenced saturation of the arterial blood with oxygen and the symptoms of dispnoea observed by the owners and episodes of apnoea and the loss of consciousness entirely subsided.
The comparative study of the acid-base balance (ABB) parameters has been performed on 20 clinically healthy mature Małopolski horses. An arterial blood sample from the facial artery and a sample of venous blood from the external cervical vein were colected from each animal. In the samples tested, the blood pH, pCO₂, tCO₂, HCO₃-, concentration of Na+, K+, Cl-, and a value of the anion gap were determined. The difference among pCO₂, tCO₂, and HCO₃ - in both samples tested was statistically significant, whereas the pH of the arterial blood and the pH of the venous blood did not differ significantly. The anion gap in both types of blood did not differ significantly. Conclusions: 1) ABB parameters such as pCO₂, HCO₃-, and tCO₂ determined in the arterial and venous blood of the Małopolski horses differ from each other significantly. 2) In spite of the lack of the differences between pH of the arterial and venous blood, the ABB parameters in horses should be determined in the arterial blood, because the comparative study performed proves that the analysis of the ABB parameters determined for the venous blood of a healthy horse may lead to a wrong diagnosis of the compensated respiratory acidosis. 3) The mean value of anion gap in horses aged 8-12 years amounts to 20.9 mmol/l for the arterial blood and 19.93 for the venous blood; the difference between the two values is not statistically significant.
Badaniem objęto 6 dorosłych owiec, którym w 2. i 3. dniu doświadczenia podawano dożwaczowo kwas masłowy w dawce 11 mmol/kg m.c./24 h. U wszystkich zwierząt przeprowadzono 4-krotne badanie krwi oraz punktatu wątroby w 1, 4, 6 i 8 dniu doświadczenia. W krwi wykonywano badania morfologiczne (Ht, Hb, Erys) oraz biochemiczne, obejmujące stężenie związków ketonowych i glukozy. Ponadto w surowicy i punktacie wątroby przeprowadzono badanie aktywności glukozo-6-fosfatazy. W wyniku przeprowadzonych badań stwierdzono zachwianie przemiany energetycznej, objawiające się obniżeniem poziomu glukozy oraz znacznym wzrostem stężenia związków ketonowych. Towarzyszyło temu zaburzenie aktywności enzymatycznej glukozo-6-fosfatazy, zarówno w surowicy jak i w wątrobie.
The studies were carried out on 20 dairy cows assigned to two groups depending on their blood serum content of ketonic compounds. The cows of group I, which had subclinical ketosis, were administered Col press supplement to feed ration, whereas the cows of group II were the control group. Col press was applied from seven days before expected parturition through four weeks postpartum. In the blood serum of all the animals, the following were determined: level of ketonic compounds, glucose, total protein and its fractions, activity of enzymes (AST, ALT, ALP and GTP) and concentration of macroelements (Ca, P inorganic, Mg, Na, K and Cl). ABB parameters were analysed in whole blood. The blood tests were done four weeks before expected parturition, and one week and four weeks after parturition.
In cattle, the prevention of diseases might be possible using dietary methods, with nutritional optimization of feed rations. For years significant influence has been associated with the addition of biotin, methionine and zinc (each given individually, or as chelate – Zn-methionine). The aim of this study was to evaluate the influence of biotin or biotin + Zn-methionine additives on the health and performance of cows, in the first and second trimester of lactation. This study was carried out in 3 groups of cows, 30 animals in each group. In the first group cows were fed the usual fodder used on the farm. In the second group biotin at 10 mg/day/cow was added. In the third group both biotin at 10 mg/day/cow and Zn-methionine at 5 g/day/cow were added. The administration of biotin increased the milk yield and reduced the occurrence of retained placenta, as well as endometritis puerperalis. However, the high doses of biotin suppressed ovulation and oestrus symptoms in the investigated cows (low progesterone levels on days 15, 21 and 45 after calving). Zn-methionine in comparison to biotin had a lower effect on the milk yield in the cows. Moreover, its supplementation improved the milk content, as well as some of the blood parameters; it also decreased the number of somatic cells in milk. The concurrent administration of biotin and zinc-methionine seemed to be a good method for the prophylaxis of subacute mastitis and for the improvement of the high yielding dairy cows’ productivity.
W badaniach doświadczalnych na szczurach określono wpływ doustnego stosowania wody dejonizowanej na niektóre parametry gospodarki lipidowej, białkowej, węglowodanowej, morfologię, rozmaz krwi obwodowej i równowagę kwasowo-zasadową we krwi tych zwierząt
W badaniach doświadczalnych na zwierzętach określono niektóre właściwości farmakodynamiczne naturalnej wody fluorkowej oraz wodnego roztworu NaF. Stwierdzono oddziaływanie tych wód na gospodarką lipidową i wodno-elektrolitową, równowagę kwasowo-zasadową oraz perystaltykę jelita cienkiego.
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