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The high level of cadmium in the abdominal muscle of the brown shrimp Crangon crangon is due to the serious pollution of the water in the Gulf of Gdańsk. The inhibition of malic enzyme (ME) activity by cadmium, and in consequence the reduced formation of NADPH, could interfere with cellular mechanisms for detoxifying the organism and reducing oxidative stress. The reduced glutathione (GSH) concentration in the abdominal muscle of C. crangon was calculated to be 5.8 mM. The objective of this study was to evaluate the part played by GSH in the effect of cadmium on the activity of NADP-dependent malic enzyme from abdominal muscles of brown shrimps. This enzyme is activated by certain divalent cations (Mg, Mn). The results demonstrate that cadmium inhibits ME activity from shrimp muscle, and that GSH and albumin can reduce this cadmium-inhibited NADP-dependent malic enzyme activity.
The content of iron, zinc, copper, manganese, lead, and cadmium was determined in the kidney, liver, and abdominal muscle of the brown hare (Lepus europaeus), a species known for its bioindicative potential in environmental quality assessment. The animals assayed were divided into immature (in their first year of life; n=25) and adult (n=39). The hares were acquired in an area situated far away from major cities and direct impacts of industrial and transport pollution. The zinc content found in the assayed hare's muscles (25.9 μg/g w.w.) and that of manganese in the kidneys, liver, and muscles (2, 2.51 i 0.85 μg/g w.w., respectively) tended to be higher than those reported from the brown hare elsewhere in Europe. The content of cadmium (particularly in the kidneys) and lead proved substantial and close to those typical of the hare inhabiting industry-affected areas. Analysis of the data pooled for all the individuals showed Zn and Cd contents to increase with age in the kidneys, the liver Cd content increasing with age as well; on the other hand, the age-Cu content was negative. In addition, a number of significant correlations between the metals themselves were revealed, particularly with respect to Zn-Cd correlation in kidneys (rs=0.47), Fe-Cd, Zn-Cu, Mn-Zn, Mn-Cu, and Mn-Pb in the liver (rs of 0.42, 0.86, 0.72, 0.79, and 0.42, respectively), and Zn-Cu in the muscle (rs=0.56). The kidney cadmium content was higher by 81% in the adult than in the immature hare, the adult hare muscle copper content being lower by 15.5%.
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