The relative abundance of carbon isotope (δ¹³C) was measured in four C₃ species (Sophora viccifolia, Quercus liaotungensis, Ostryopsis davidiana and Zizyphus jujuba var. spinosa) of the Loess Plateau in China from the 1930’s to 2002. The results showed that the δ1¹³C values in the four species varied from -25.05 %o to -29.75 %o with their average at -27.04 %o. A decrease in the δ¹³C value with time was found in all the four species, which indicating that the water use efficiencies (WUEs) of all the measured species declined during 70 years. However, the decrease in δ¹³C value differed among the four species with its significant decreases measured in two of the species, Sophora viciifolia and Quercus liaotungensis, its relatively significant decrease found in Ostryopsis davidiana, and its slight decrease appearing in Zizyphus jujuba var. spinosa. in the δ¹³C values in the four species decreased by 14.65 %o, 14.46 %o, 11.99 %o and 2.44 %o, respectively. The different species were shown to have different sentitivtties to climatic change, and Zizyphus jujuba var. spinosa was found to be the most drought-tolerant species of the four, which had a high WUE.
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