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A field experiment was conducted in the years 2003-2004 on a field of 10-years’ soybean monoculture, with naturally accumulated infection material in the soil. Soybean of ‘Mazovia’ was the object of studies. Before the sowing the seeds were dressed, and at the beginning of anthesis the plants were sprayed with biopreparations Polyversum, Biosept 33 SL and Biochikol 020 PC. The experiment considered the combination with dressing the seeds by means of Zaprawa Oxafun T (a.s. carboxine 37.5% + tiuram 37.5%) and spraying the plants with fungicide Bravo Plus 500 S.C. (a.s.: chlorotalonile 50%). The seeds that were not dressed constituted the control. During the vegetation the number and healthiness of soybean plants were determined twice (in the seedling phase and at anthesis), at the same time a laboratory mycological analysis was carried out. After the harvest the studies established the size and quality of the seed yield of soybean growing in particular experimental combinations. The best number of plants was obtained in the combination with Biosept 33 SL, slightly weaker after applying Polyversum or Zaprawa Oxafun T, and the worst in the control combination. Biosept 33 SL and the fungicide were the most effective in protecting the plants from infection by soil-borne fungi. Polyversum and Biochikol 020 PC also showed a good protective effect. The greatest seed yield was collected from soybean plants after applying Biosept 33 SL, while the lowest – from control plants. Soybean plants were mainly infected by Fusarium spp.,A. alternate, P. exigua, R. solani and P. irregulare. Those fungi were most frequently isolated from control plants, and the least frequently after applying Biosept 33 SL or Polyversum. The proportion of pathogenic fungi (P. sojae, Fusarium spp., S. sclerotiorum, P. exigua) in seed infection of soybean was the smallest in the combinations with Biosept 33 SL or Zaprawa Oxafun T, while the biggest in the control.
This study presents the effect of biopreparations (Polyversum, Biochikol 020 PC and Biosept 33 SL) on fungal and bacterial rhizosphere and non-rhizosphere communities after seed dressing and spraying of Phaseolus vulgaris plants. The use of biopreparations has a positive effect on the communities of bacteria and fungi in soil under the cultivation of this plant. The number of cfu of the studied microorganisms in the nonrhizosphere soil was slightly lower than in the rhizosphere. Biochikol 020 PC and Biosept 33 SL increased the number of cfu of bacteria Bacillus spp. and Pseudomonas spp. and decreased the population of soil-borne fungi. Different species were isolated within the fungi and they belonged to the following genera: Altenaria, Fusarium, Rhizoctonia, Sclerotinia, Gliocladium, Penicillium and Trichoderma. The most antagonistic bacteria and fungi were obtained after introducing biopreparations Biochikol 020 PC or Biosept 33 SL. The smallest number of antagonists were found in the soil after dressing the bean seeds with Zaprawa Oxafun T and spraying the plants with fungicide Bravo Plus 500 SC and in the control combination. Besides, the applied biopreparations and fungicides had a positive effect on Pheseolus vulgaris yielding.
The studies conducted in a growth chamber observed the protective effect of biopreparations such as Biosept 33 SL, Biochikol 020 PC and Polyversum, used as seed dressing for common bean and pea. The fungicide Zaprawa Oxafun T was used as a comparison, while the seeds that were not dressed constituted the control. The studied plants grew in the medium grown over with different species of fungi pathogenic towards pea and common bean. Irrespective of the species, the fewest plants, with the greatest proportion of infected ones, were obtained in control combinations. The application of biopreparations and the fungicide turned out to be an effective protection of pea and common bean from the infection by the examined phytopathogens. Biosept 33 SL in the concentration of 0.2% showed to have the best effect among the tested biopreparations.
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