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The chemical composition of Garcinia kola seed and hull was determined using standard methods. Results show that crude protein, lipid extract, ash, and crude fibre ratios are: 39.52 and 99.92 g/kg, 43.25 and 42.91 g/kg, 11.42 and 18.62 g/kg, 114.02 and 153.44 g/kg respectively. Carbon : nitrogen ratios for the seed and hull are 57.88 and 29.01, respectively. Garcinia kola hull had significantly higher (p<0.05) protein and fibre but has comparable values for lipid and ash. Potassium and phosphorus were the most abundant mineral elements in Garcinia kola seed (334.82 and 242.61 mg/kg, respectively) while phosphorus predominates in the hull (288.61 mg/kg). The seed had significantly higher values for sodium, potassium, copper and cobalt while chromium, molybdenum, manganese, nickel, selenium, lead and mercury were not detected. The dominant saturated, monosaturated, and polyunsaturated fatty acid, in the seed and hull are palmitic (31.55 and 276.01 mg/kg), oleic (38.36 and 52.77 mg/kg) and linoleic acids (36.16 and 235.83 mg/kg) respectively. The hull has significantly higher (p<0.05) α-linolenic acid content. Glutamic acid is the dominant non-essential amino acid in seed and hull (6.80 and 8.10 g/kg, respectively) while lysine and valine (2.40 and 7.10 g/kg, respectively) are the dominant essential amino acids. The proportion of essential amino acid in the total amino acid is 44.52% in the hull and 35.81% in the seed. Garcinia kola seeds and hulls may find use in food and feed formulations by virtue of their chemical composition.
Diarrhoea, dysentery and other diseases due to other enteric bacteria have reportedly been found to resist chemotherapeutic treatment in some West African communities with fatal consequences in some cases. This study was carried out to determine multidrug resistance patterns of Enterobacteria isolates from processed ready-to-eat foods. Indigenously processed food samples of different types were collected from two Francophone and two Anglophone countries in the West African sub-region during the wet and dry seasons of a sampling period of two years. Enterobacteria were isolated from the samples using standard techniques. Amplification of chromosomal DNA of the isolates using the Polymerase Chain Reaction was carried out. The results obtained were subjected to statistical analyses. All isolates showed resistance to cefuroxime (90.7%), nitrofurantoin (90.6%), augmentin (86.1%) and ampicillin (51.2%) while all were sensitive to gentamycin and ciprofloxacin. There was amplification indicating the presence of invA gene at a position of 240 bp. There was no amplification at all for the spvC gene in any of the isolates tested. Multidrug resistant enteric bacteria in these foods containing the invA gene could lead to infections with uncontrolled antibiotic use. The presence of enteric bacteria in the foods analyzed which provide undeniable evidence of the poor microbiological quality of these foods could form the basis of a useful databank in formulation of food-borne disease control and prevention strategies.
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