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The presence of symplasmic isolation and symplasmic continuity which are functional aspects of cell-to-cell communication, had been studied in cambium of Acer pseudoplatanus and Ulmus minor, with hope that uniqueness of this meristem, exemplified by its morphology and seasonal variations in its activity is also manifested in differences in the efficiency of communication between cambial cells during the year. The degree of symplasmic continuity was estimated by loading the fluorescent symplasmic tracer to the stem and following its distribution in a population of cambial cells observed on tangential, transverse and radial sections. In active cambium the tracer did not enter the rays. This suggested that the ray and fusiform cells, growing and dividing intensively at different rates were specifically isolated from each other. In the state of dormancy the tracer was present also in the rays implying continuity between the two types of cambial cells. Temporal restriction in tracer spreading from secondary xylem to cambial region was observed on transverse sections in both physiological states of the meristem. Higher degree of symplasmic isolation in active cambium is, most probably, associated with functional distinctiveness of ray and fusiform cells. We hypothesize further that the symplasmic continuity in dormant cambium results from the open conformation states of plasmodesmata, because the energy costs of these states are low. It is reasonable strategy when cambial cells do not divide and maintenance of their functional individuality is not necessary.
Field elm (Ulmus minor Mill.) is distributed mainly across central and southern Europe. In Poland this species occurs in the lowlands and foothills, where it grows mainly in the floodplain forests along the rivers. U. minor exists in a variety of climatic and ecological conditions. It is capable to tolerate floods as well as drought. Currently, most populations of U. minor are small and fragmented resulting from human activity and Dutch elm disease. Moreover, in the natural field elm populations, vegetative propagation by root suckers or sprouting can be observed. All these factors may affect the level of genetic variation of U. minor populations in Poland. In the present study, we determined the level of genetic variation and the clonal diversity of twelve natural U. minor populations in Poland (407 individuals) using eight nuclear microsatellite loci. The obtained results indicate that the studied field elm populations are characterized by low level of genetic variation (He=0.382; Ho=0.555; A=7.0). Additionally, the high level of clonality in field elm populations was estimated. The clonality level of examined elm populations varied among them, and in some cases was very high. Out of the 407 individuals analysed for clonal structure only 61 multilocus genotypes were identified. Furthermore, only one genotype was identified in the three study populations of field elm, which means that in each of these populations all trees belong to one genet. The values of genotypic richness (R) were heterogeneous among populations, with mean 0.148. The knowledge on the genetic diversity and the clonal structure of U. minor populations is essential to make future decisions regarding conservation of genetic resources of this species in Poland.
Three elm species are native to Poland: wych elm (Scots elm) (Ulmus glabra Huds.), field elm (U. minor Mill.), and European white elm (fluttering elm) (U. laevis Pall.). The epidemic of Dutch elm disease (DED) has led to a decrease in the popularity of elm cultivation. An analysis of forestry data was the first step in the assessment of elm resources. The area of forest stands where elms are dominant has more than doubled since 1978. Lowland alluvial forests rank first in regards to the number of elm localities per unit area of a given forest site type. While this site type is clearly preferred by elms, the majority of elm trees are actually scattered among sites of oak-hornbeam or closely related forests (of the alliance Carpinion betuli). Field research revealed a clear dominance of U. laevis, a species which in the past was predominantly located out of woodland and rarely cultivated. Data analysis indicated that all trees greater than 70 cm in diameter belonged to this species. Data from plots surveyed directly also suggest that the three elm species have slightly different habitat preferences. U. laevis prefers riparian habitats, although the major part of its resources is now on potential sites of oak-hornbeam or closely related forests. U. minor even more often than U. laevis occurs at less humid sites (mostly potential sites of oak-hornbeam or closely related forests), while U. glabra prefers moist slopes. In general, it appears that the impact of DED in the last 20–30 years has been smaller than in the preceding period, however, the disappearance of the disease has not been established. Undoubtedly, U. laevis is the elm species that is least impacted by DED
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