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The goal of present work was to investigate wool quality of camels breeding in the Tunisian part of Sahara Desert. The study was carried out with 10 camel males at the age of three years. The samples of wool were taken from the left mid-side of a shoulder. Due to the low regrowth rate of wool, the samples were not split into the external and internal fractions before a measurement. The individual fibers were classified into three groups: 1) fibers with continuous medulla, 2) fibers with intermittent medulla, 3) fibers without medulla. Then the percentage of each type of fibers was evaluated. Thickness measurement was made using the microprojection method according to Polish Standard PN-72/ /P-04900. At least 600 fibers were measured in each sample. The high fraction of hair from the core constant rate of 78% wool with a thickness of up to 48 |im, and the hair thickness variation of 47% be attributed to the roughness of the wool. The wool of the camels under study should be used for the production of carpets and handicrafts. The occurrence of continuous core both in the thin and thick fibers confirmed the adaptation of camels to a large diurnal temperature variation, as in llamas and alpacas.
Thirteen Cuvier’s gazelles were relocated to a 6-ha acclimatization enclosure in Boukornine National Park (Boukornine NP) in Tunisia, where they are part of a reintroduction project. To determine the degree of adaptation and habitat use under the new conditions, the acclimatization enclosure was divided into 6 sections according to topography, plant cover and plant species in the area. Signs of gazelle activity were coded as feeding site, paths, passages, feces and resting places. Sampling was done in spring, summer and autumn from September 2000 to July 2001. Multivariate analysis using PATN analysis and Χ2 distribution tests were used to analyze the data. Multivariate analysis yielded 5 groups of biotopes according to the above variables. The Χ2 distribution test showed the significant effect of each variable on the presence of signs of gazelles. Cuvier’s gazelles prefer areas with low and west to north facing slopes and scant plant cover; animals are attracted to the proximity of the fence as the limit of their territory and even though the presence of humans does not represent a disturbance, gazelles select areas far (> 50 m) from the supplementary feeding and water supply for their activities.
A recent paper on the occurrence of the genus Crocidura in Tunisia reports a single specimen identified as C. suaveolens. Therefore a third species, besides C. russula and C. whitakeri would occur in the country. However, the presence of C. suaveolens in North-Africa is controversial and was recently ruled out from the other Maghrebi countries (Algieria and Morocco). During the period 1989 - 90, 71 specimens of shrews were collected from owls pellets or trapped at Tunisian 12 sites. This material was measured and studied both by classic morphometric and multivariate methods (Fuzzy test, Principal Coordinate Analysis and Generalized Procrustes Analysis), considering also reference samples (C. suaveolens from Italy and Balearic Islands, C. russula and C. whitakeri from Morocco). Four of the 12 trapped shrews were karyotyped and resulted to have a C. russula karyotype, some differences in biometry as well as in Number of Fundamental arms (NFa) separate the Moroccan population from the Tunisian one and raise some interesting questions on the taxonomy of C. russula. Multivariate analysis also allowed us to exclude the presence of C. suaveolens from the Tunisian material studied and suggested also the misclassification of the proposed specimen that should be reconsidered as C. whitakeri. A preliminar distribution map of the two species living in Tunisia was drawn, also based on the scanty data from the literature.
The genetic diversity within and among nine natural populations of Thymus hirtus Willd. subsp. algeriensis (Boiss. et Reut.) Murb. from different geographical and bioclimatic zones were assessed using Random Amplified Polymorphic DNA data. A total of 154 bands were generated from seven selected primers. 141 bands were polymorphic (P=91.56%). The genetic diversity within a population based on Shannon's index which was high (H'pop=0.307) and varied according to bioclimatic zones. A high genetic differentiation among populations (GST=0.335 and FST=0.296) was revealed, suggesting a population isolation and a low level of gene flow among them. The major proportion of the variation was attributable to individual differences within populations. The genetic structure is in accordance with geography distances. The Neighbour- joining tree based on Nei's and Li's genetic distance among individuals showed that individuals from each population clustered together. The UPGMA dendrogram based on FST values revealed three clusters each of them includes populations closuring to their geographical origin. The high genetic structure of populations resulted from genetic drift caused both by the habitat fragmentation and the low size of most populations. The in situ conservation should interest all populations. The ex situ conservation should be based on the collection of seeds rather within than among populations because of the maximum of variation was revealed within populations.
In this study, a total of 54 Vibrio alginolyticus strains were analyzed. The isolates were recovered from different compartments of the Ruditapes decussatus hatchery in the National Institute of Marine Sciences and Technologies, Monastir, Tunisia. All isolates were biochemically identified (API 20E and API ZYM strips), characterized by amplification of the Hsp-40 gene polymerase chain reaction (PCR) and analyzed by enterobacterial repetitive intergenic consensus (ERIC)-based genotyping to evaluate genetic relationship between the isolated strains. We also looked for the presence of ten V. cholera virulence genes (toxRS, toxR, toxT, toxS, tcpP, tcpA, ace, vpi, zot and ctxA) in the genomes of Vibrio isolates. The antibiotics susceptibility, exoenzymes production and in vitro cytotoxic activitiy against HeLa cell line were also carried out for all tested bacteria. Most of V. alginolyticus isolates showed significant antimicrobial resistance rates to at least ten antibacterial agents. For most isolates, the minimum inhibitory concentration (MIC) data showed that tetracyclin and streptomycin were the most effective antibiotics. Construction of the phylogenetic dendogram showed that studied isolates were in general genetically heterogeneous; however some Vibrio strains were present in different structures of the R. decussatus hatchery. The V. cholerae virulence genes investigation showed a wild distribution of toxS (49/54), toxR (45/54) and toxT (22/54) genes among V. alginolyticus strains isolated from the R. decussatus rearing system. Cytotoxic effects of several Vibrio extracellular products (28/54) were also observed on HeLa cells.
The present study reports the levels of mercury and selenium in Sarpa salpa and Balistes capriscus collected along the coast of Mahdia and Sfax (Tunisia). The systems constituted by S. salpa and Robphildollfusium fractum and by B. capriscus and Neoapocreadium chabaudi were tested as potential bioindicators to monitor environmental Hg pollution in marine ecosystems. Mercury and selenium concentrations were assessed in kidney, liver and muscle of 51 S. salpa and of 45 B. capriscus as well as in their respective endoparasites R. fractum and N. chabaudi. The Se:Hg molar ratios were evaluated for both species across the study areas. Surprisingly, the Se:Hg molar ratio in B. capriscus muscle from Mahdia is significantly lower than in Sfax. Our results indicate that some parasites may also be implicated in the amount of Se and Hg available in tissues and therefore contribute to oscillations of the Se:Hg molar ratios. In the model involving the carnivorous species (B. capriscus), the 5.1-times higher levels of mercury in N. chabaudi than in B. capriscus muscle in Sfax enable this fluke to be a sensitive biomonitoring tool for Hg pollution. The present results confirm that the habitual consumption of S. salpa should not suppose any potential health risk for Tunisian people. On the other hand, the consumption of B. capriscus may be of concern and further monitoring is advisable, since the Hg average concentration in Mahdia was above the maximum allowed Hg concentration in the edible portion of fish fixed by the European Union.
Lecithostaphylus tylosuri sp. nov. (Digenea, Zoogonidae) specimen were collected from the digestive tract of Tylosurus acus imperialis (Teleostei, Belonidae) caught off the eastern coast of Tunisia. L. tylosuri is very similar to its closest relatives, L. retroflexus and L. nitens. It can be easily distinguished from L. retroflexus (Molin, 1859) in having a more extensive vitellarium, with follicles reaching from the posterior margin of the acetabulum and extending beyond the posterior margin of the testes and a coiled seminal vesicle. L. tylosuri differs from L. nitens as illustrated by Linton 1898, in having a longer cirrus pouch (0.7 mm vs 0.36 mm, respectively) overlapping the anterior edge of the ventral sucker and a submarginal genital pore (submedian in L. nitens). It’s also different from L. nitens as described by Manter 1947 in the vitelline disposition and in having the greater sucker ratio (1: 1.3–2.1 vs 1: 1.3–1.6, respectively). L. tylosuri differs from L. nitens as reported by Machida and Kuramochi 2000 by the absence of variations in the vitellarium disposition in all specimens. L. tylosuri is more similar to L. nitens from group A (considered synonym of L. ahaaha Yamaguti, 1970 = L. nitens by Bray 1987) by having vitelline follicles extending beyond the testes. L. tylosuri can be distinguished from L. ahaaha by its pedunculate rather than prominent acetabulum and its larger body size (4.10–7.85 mm long and 0.75–1.2 mm large vs 2.1–6 mm long and 0.45–1.1 mm large, respectively). The prevalence of L. tylosuri sp. nov. was negatively correlated with host length (decreasing with host size increasing). Host sex does not seem to affect infection parameters.
In Tunisia, Thymus capitatus L. populations are severely destroyed due to deforestation and over-collecting. The species occurs in small scattered populations decreasing progressively in size. Yet, no conservation or improvement programs are attempted to preserve and promote the potential value of this resource. In this work, we assessed the genetic diversity of nine Tunisian populations of Thymus capitatus L. from different bioclimates, using 103 polymorphic randomly amplified polymorphic DNA (RAPD) loci. The analysis of the genetic variation within and among populations is primordial to elaborate conservation and improvement programs. The species showed a low diversity within populations (0.276
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