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The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X-100

100%

Panasiewicz M., Domek H., Hoser G., Fedoryszak N., Kawalec M., Pacuszka T.

Cellular and Molecular Biology Letters

2009

tom 14

nr 2

175-189

Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [3H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C14, C18 or C18:1 fatty acids (Fas)

Effect of detergents on xanthan production during batch and continuous cultivation of Xanthomonas campestris NRRL B-1459

80%

Janas P., Gustaw W., Mleko S., Pielecki J.

Acta Scientiarum Polonorum. Technologia Alimentaria

2003

tom 02

nr 1

The effect of the addition of detergents Tween 20, Tween 40, Tween 80 and Triton X-100 on xanthan production was investigated during batch and continuous cultivation of Xanthomonas campestris NRRL B-1459. Three of the four tested detergents: Tween 20, 40 and Triton-X 100 gave an increased xanthan production in comparison to control cultivation without detergent in the medium. The best results were achieved either with 0.05% or 0.1% concentrations of Triton-X 100. About 1.18 to 1.21 fold higher production of polymer was observed during batch cultivation in the presence of this compound in the medium. The highest xanthan concentrations were observed on day 5 and 4 of continuous cultivation in the presence of 0.05% and 0.1% of Triton X-100 (respectively 1.34 and 1.36 fold higher than control). Toxic effect of 0.1% Triton X-100 on the cells growth of the strain was observed after 5 days of continuous cultivation. In addition many examples of effect of detergents on the production of biotechnological useful compounds by various microorganisms have been presented in the work.

Ograniczanie wyników

1 Acta Scientiarum Polonorum. Technologia Alimentaria

1 Cellular and Molecular Biology Letters

1 Domek H.

1 Fedoryszak N.

1 Gustaw W.

1 Hoser G.

1 Janas P.

1 Kawalec M.

1 Mleko S.

1 Pacuszka T.

1 Panasiewicz M.

1 Pielecki J.

1 2009

1 2003

Wyniki wyszukiwania

The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X-100

Effect of detergents on xanthan production during batch and continuous cultivation of Xanthomonas campestris NRRL B-1459