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Infekcje Toxocara w swietle materialow wlasnych

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Toxocarosis clinical forms wers diagnosed according to KRAUS et al. (1995). There were three forms: l) asymptomatic - marked by hypereosynophilia and ELISA positive serologic reaction, 2) minor - with cutaneous, pulmomary and pseudorheumatic lesions, 3) major – with hepatosplenomegaly and multifocal inflammatory lesions of organ or sight. Over the years of 1994-1997 in Infectious, Parasitic and Tropical Diseases clinic of the Voivodship Specialist Hospital of Łódź we found and treated 137 Toxocara canis cases. Among them 63 (46.0%) asymptomatic, 57 (42.6%) minor and 17 (12.4%) major forms were diagnosed. The disease was detected in 80 (58.4%) adults and 57 (41.6%) children. Seventy six patients lived i towns and 61 in country. Minor form patients had the symptoms as follows: skin allergy, large joints lesions, augmentation in lymph nodes. Radiology examinations revealed pulmonary lesions. Using ultrasonography there was found hepatosplenomegaly and changes echogenity in patiems with major form. Biochemical tests showed elevation in hepatic enzymes activity. Lesions of organ of sight were multifocal and usually included uveitis, retinitis, inflammation of anterior chamber and inflammation of vitreous body.
The biology of the ascarid nematodes has been discussed in the context of their important economic role in farm animals, pet animals and zoo animals with special attention to carnivores and primates. In farm animals, infection with the most common roundworm of horses (Parascaris equorum) and swine (Ascaris suum) depend on many factors such as environmental conditions (larval development in the egg and egg survival), age of the host, breed, husbandry system, hygiene and treatment schedule. The monoxenic ascarids Toxocara canis and T. cali are the most important nematodes in carnivorous animals (dogs, cats, foxes) and carnivores in the zoo. In the period of March 2000 till March 2001, 57,1% of examined representatives of Felidae, Ursidae and Canidae in the Zoological Garden of Wroclaw were found to be infected with ascarids (T. canis, T. cati, Toxascaris leonina). The prevalence of T. canis in the Canidae was 66,7%, of T. cati in the Felidae was 14,3%, while 57,1% of the Felidae carried T. leonina infection. Ascaris lumbricoides, a typical parasite of primates, was found in some gorillas, chimpanzees and orang-utans during parasitological survey.
The differentiation of T. canis and T. cati eggs is an important problem in epidemiology. It has been demonstrated that it is possible to differentiate the species of Toxocara spp. eggs using light microscope and evaluating the morphological features of the eggs. The size of the eggs and their shells appearance are very characteristic. T. canis eggs prevail in larger size classes than T. cali and have thicker, less permeable for light and more pleated shells. Using such criteria we were able to differentiate the species of the majority of Toxocara spp. eggs found in soil.
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Toxocarosis is still an important and actual problem in human medicine. It can manifest as visceral (VLM), ocular (OLM) or covert (CT) larva migrans syndroms. Complicated life cycle of Toxocara, lack of easy and practical methods of species differentiation of the adult nematode and embarrassing in recognition of the infection in definitive hosts create difficulties in fighting with the infection. Although studies on human toxocarosis have been continued for over 50 years there is no conclusive answer, which of species - T. canis or T. cati constitutes a greater risk of transmission of the nematode to man. Neither blood serological examinations nor microscopic observations of the morphological features of the nematode give the satisfied answer on the question. Since the 90-ths molecular methods were developed for species identification and became useful tools being widely applied in parasitological diagnosis. This paper cover the survey of methods of DNA analyses used for identification of Toxocara species. The review may be helpful for researchers focused on Toxocara and toxocarosis as well as on detection of new species. The following techniques are described: PCR (Polymerase Chain Reaction), RFLP (Restriction Fragment Length Polymorphism), RAPD (Random Amplified Polymorphic DNA) and SSCP (Single Strand Conformation Polymorphism).
Sixty three children, who were living in an area known to be heavily contaminated by dog faeces in the centre of city of Poznań, Poland (Toxocara spp. eggs were found in 53% of 40 g soil samples), were examined for antibodies to Toxocara spp. Five children (7.9%) had high absorbance values in the Toxocara ELISA test. Geophagia and dog’s ownership were the major associated risk factors. Neither present nor past symptoms and signs usually related to toxocarosis were found in any of the seropositive children. The study confirmed that toxocarosis may be clinically inapparent even in an area where exposure to infection is common and serological responses well pronounced.
The objective of this study was to investigate the ascarid infection in Asiatic lions using scat samples, based on microscopic analysis, PCR amplification of the ITS-2 region of ribosomal DNA and sequence analysis of the amplicons. Microscopic analysis indicated the presence of eggs of Toxascaris leonina in eleven of the sixteen scat samples analysed and in one of these eleven scats eggs of Toxocara cati were also detected. In five of the scats eggs were not detectable. The presence of T. leonina in all the infected samples was also confirmed by PCR amplification of the ITS-2 of ribosomal RNA gene and five of these also showed amplicons corresponding to T. cati, respectively. Toxocara canis infection was not observed in any of the scat samples. Nucleotide sequence analysis of the ITS-2 region indicated 97% to 99% similarity with T. leonina and T. cati, respectively. To our knowledge, this is the first molecular characterization of ascarid infection in captive Asiatic lions from a zoological garden of India. This study also indicates that Asiatic lions are more prone to infection either with T. leonina or T. cati and the parasite is not host specific.
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