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Wysoka cena wody oraz okresowe jej niedobory skłaniają do poszukiwania nowych technologii produkcji. Jedną z możliwych alternatyw mogą być super-absorbenty czyli hydrożele. Są to różnej natury chemicznej polimery mogące chłonąć znaczne ilości wody. W niniejszej pracy badano właściwości hydrożelu Alcosorb (AS 400). Dokonano także oceny jego przydatności jako komponentu podłoży ogrodniczych. Jako roślinę testową wybrano aksamitkę (Tagetes erecta fl. pl. “Alaska”). Stwierdzono, że Alcosorb ogranicza wymywanie składników z podłoża, minimalizuje stres wodny roślin podczas okresowych niedoborów wody, wpływa na zmniejszenie zużycia wody niezbędnej do podlewania roślin.
Tissue cultures of Tagetes erecta Linn. were initiated from leaves and flowers on MS and RT media with various cytokinins and auxins combinations Cultures were maintaned at 25°C, light intensity 3000 lux and photo-period 16 h. Obtained callus tissue was transferred on regeneration medium (MS plus various combinations of growth regulators) for differentiation. The best organogenesis was observed on MS plus BA 5 mg/dm3 plus IAA 3mg/dm3 medium. Rooted plantlets were transferred to sterilized soil. Regeneration of plant from leaves tissue culture was not successful. The presence of pyrethrins in callus was detected.
This study was aimed at evaluating the effect of sublethal doses of glyphosate on physiological parameters of a common ornamental plant Mexican marigold (Tagetes erecta). The herbicide was applied in the following doses: 720 g ⋅ ha–1 (standard field dose), 144 g ⋅ ha–1, 28.8 g ⋅ ha–1, and 14.4 g ⋅ ha–1, in the form of a spraying treatment of plants in a specialist spraying chamber. The net assimilation rate and leaf greenness index were then determined. Herbicide application in the sublethal doses, i.e. below 720 g ⋅ ha–1, caused disorders in both analyzed physiological parameters of plants. The glyphosate dose of 144 g ⋅ ha–1 elicited transient disorders in the leaf greenness index. In turn, the use of the lower doses (28.8 g ⋅ ha–1 and 14.4 g ⋅ ha–1) caused a short-term increase in the net photosynthesis rate in the plants which was accompanied by a decreased value of the leaf greenness index. Study results demonstrated the effect of sublethal doses of glyphosate as a stress factor in parameters associated with the process of photosynthesis in plants.
In the spring-summer season in the years 2005 and 2006 (every year) three vegetation experiments were carried out with three species of ornamental plants: common sunflower (Helianthus annus ‘Pacino’), scarlet sage (Salvia splendens ‘Fuego’), tagetes erecta (Tagetes erecta ‘Inca Yellow’). These plants were planted in a substrate artificially contaminated by cadmium. Doses of cadmium applied in the experiment represent different degrees of contamination. The dose of 1 mg Cd ∙ dm⁻³ indicates natural contents; 5 mg Cd ∙ dm⁻³ – small contamination; 10 mg Cd ∙ dm⁻³ – large contamination. The objective of the presented studies was the determination what quantities of cadmium pass from the substrate to the organs of the studied plants and which plant organs accumulate the greatest amounts of cadmium. Cadmium was mainly accumulated in leaves and shoots, then in inflorescences. While the least amount of this metal was found in the roots with the exception of Tagetes erecta where the greatest amount of cadmium was found in roots, then in leaves and shoots, while the lest amount was in inflorescences. The greatest cadmium contents were found in the roots of Tagetes erecta, in leaves and shoots of Salvia splendens, and in the inflorescences of Helianthus annus. Among the studied ornamental plant species, the plants of Tagetes erecta were characterized by the highest cadmium uptake.
Biocontrol of plant diseases has emerged as an eco-friendly measure of plant protection and has experienced a lot of devotion in the last two decades. Biocontrol agents include application of microbial agents, their secretion products and natural extracts from different parts of several plants. The present study, therefore, aimed at evaluating the potency of aqueous extracts of Tagetes erecta L. (marigold) in controlling bacterial speck disease in tomato plants. The experimental design consisted of two groups of 50 plants each: group 1 – sprayed with sterile water (control); and group 2 – sprayed with marigold extract. Spraying was performed under aseptic conditions at the third node from the base of each plant. Challenge inoculation with the bacterial speck pathogen Pseudomonas syringae pv. tomato was performed to analyze the disease severity on the test plants. The parameters of study were analysis of alteration in the activity and gene expression of peroxidase (POX), phenyl ammonia lyase (PAL), and polyphenol oxidase (PPO), as well as isoform expression of POX and PPO. The results demonstrated strong inductive effects of the extract on the activity and genes of POX, PAL and PPO. De novo expression of POX and PPO isoforms following marigold extract treatment was also observed. The observations indicate that marigold extract could be a promising biopesticide.
Sugar mill is one of the agro based industry and great significant in rural economy of developing countries. It creates environmental pollution by produced waste during sugar production. In the present work deals with the analysis of various change of biochemical in African marigold (Tagetes erecta L.) at 15 DAS under the different concentrations (control, 10, 25, 50, 75 and 100%) of sugar mill effluent. Results of this study determined that the lower concentration (10%) of sugar mill effluent increased photosynthetic pigment and biochemical contents of African marigold and it decreased at higher concentrations of the sugar mill effluent. The lower concentrations of sugar mill effluent used for irrigation.
The present investigation has been carried out on Tagetes erecta to search out the effect of sugar mill effluent on growth and yield. In-vivo conditions were set up for the experiment purpose. Pots were filled with soil and treated with different concentrations of sugar mill effluent i.e., 10, 25, 50, 75 and 100% on morphological parameters of test crop. Results of pot culture experiment showed that at 10 % concentrations of sugar mill effluent proved to better to the crops growth and thereafter the growth may reduce over control. In order to mitigate the effluent toxicity certain Biofertilizers inoculants were mixed to the soil along with the same effluent treatments. The study suggests that the Biofertilizers inoculants along with effluent irrigation proved to be better for plants growth.
The objective of the presented studies carried out in the Department of Horticultural Plant Fertilization, University of Life Sciences in Poznań was the investigation of the influence of the introduced doses of cadmium and lead (soil pollution) on the yield of the particular organs of selected cultivars of Marigold (Tagetes erecta L.) and to check whether these plants are suitable to be planted on soils polluted with the mentioned metals. Plants used in the experiment were planted in individual outflowless containers in plastic tunnel. The studied cultivars of Marigold were planted in a substrate artificially polluted with cadmium and lead. The two heavy metals were introduced in the following doses: Cd – 0 (control); 1; 5; 10 mg dm⁻³, Pb – 0 (control); 100; 500; 1000 mg dm⁻³. Statistical analyses carried out in the present paper refer to the analysis of variance for leaf, stalk and inflorescence matters and the number of inflorescences. Analysis of the mean total yield of the particular cultivars grown in all substrates has shown that the highest total yield was reached by ‘Titania’ cultivar, in the second place was ‘Hawaii’ and the smallest yield was obtained from ‘Mann im Mond’. The smallest amounts of cadmium and lead, regardless of the applied dose, remained in the substrate in which ‘Hawaii’ cultivar was grown.
In the Department of Plant Fertilization, University of Life Sciences in Poznań, a vegetation experiment (in plastic tunnel) was carried out in which the substrate was artificially contaminated with cadmium and lead and three cultivars of Tagetes erecta L. were planted in it: ‘Mann im Mond’, ‘Hawaii’ and ‘Titania’. The substrate was artificially contaminated with increasing doses of metals: Cd – 0 (control); 1; 5; 10 mg dm⁻³; Pb: 0 (control); 100; 500; 1000 mg dm⁻³. The objective of studies was to investigate which organs of Tagetes erecta L. cumulated the greatest amounts of cadmium and lead, what amounts of Cd and Pb move from the substrate to the organs of the studied cultivars and whether the studied cultivars of Marigold are suitable for the remediation of soils polluted with cadmium and lead. The metals contained in the particular plant organs, after dry mineralization, were identified by the method of atomic spectrophotometer AAS-3 of Zeiss Company. In all studied cultivars, the highest contents of cadmium were found in leaves, smaller amounts were shown in stalks, while inflorescences contained the smallest Cd amounts. The highest amounts of lead in all studied cultivars were found in the stalks, smaller amounts in the leaves and the least amount was shown in the inflorescences The greatest contents of cadmium and lead were found in the organs of ‘Hawaii’ cultivar, both in the leaves, stalks and inflorescences. The studied cultivars of Tagetes erecta can be utilized for the remediation of soils polluted by heavy metals.
Although chemical priming agents have been successfully used to improve seed germination, emergence and seedling quality in different plant species, still they are not yet useful for organic cultivation. Marigold is a multipurpose plant and rich source of lutein and gallic acid, to be explored as an organic priming agent. The purpose of this study was to identify an organic priming agent for organic growing and to develop appropriate methods by using two different species of marigold (Tagetes patula L. and T. erecta L.). Herbal tea obtained from the dried flower of marigold was used as a priming agent for eggplant (Solanum melongena L) cv. Pala seeds. Hydropriming and control seeds were compared with organic priming (T. patula and T. erecta) for germination percentage, mean germination time, germination rate, two different emergence tests, seedling height, seedling fresh and seedling dry weights. Data obtained from the application of organic priming (T. patula and T. erecta) showed a difference in both the germination (82 and 80%), and the emergence (73 and 76%) from Controls and Hydropriming. Seeds emerged in, which unprimed in 52 and 39% respectively, when primed with T. patula, emerged in 73 and 56% respectively. The mean germination time and the mean emergence time were shortened compared with Controls and Hydropriming. MET decreased in 1 cm and 2.5 cm depth sowing from 14.0 and 16.1 days than 10.3 and 11.9 days in Emergence-1 and 10.4 and 10.2 days in Emergence 2, respectively. Seedling quality characteristics were examined in the seedling height, the seedling fresh and dry weights of T. patula and T. erecta applications were also statistically significant. This is the first report which results showed that marigold species can be used as a potential organic priming agent.
In Poland, cultivars belonging to three species of Tagetes are grown: T. erecta, T. patula, T. tenuifolia. Presented studies were conducted in 2015 in two localities of the Lublin region. Those studies included cultivars of Tagetes spp.: T. erecta (cv. ‘Alaska’, ‘Hawaii’, ‘Mann Im Mond’), T. patula (cv. ‘Bolero’ and ‘Carmen’) and T. tenuifolia (cv. ‘Lemon Gem’ and ‘Lulu’). Six weeks after the sowing and in flowering stage of analyzed marigold cultivars, the plants healthiness was assessed. The mean values of the disease index ranged from 16.25 to 29.0. The main cause of root infection at the seedling stage proved to be the species of Fusarium and Rhizoctonia solani. Proportion of plants with disease symptoms in flowering stage ranged from 1.25 to 5.5%. Regardless of the place of cultivation, species: A. alternata, F. culmorum and F. equiseti were the most often isolated from diseased plants in the flowering stage. Studies on the susceptibility of analyzed cultivars of marigold to infection by Fusarium culmorum, F. equiseti and F. sporotrichioides were conducted in a growth chamber. Significantly, the lowest value of the disease index of cultivar ‘Bolero’ (T. patula) allows to accept that cultivar as the least susceptible to infestation by Fusarium spp. under conditions of controlled temperature and humidity.
The present study aimed to isolate and identify the chlorpyrifos resistant soil bacteria from contaminated soils in order to be used for bioremediation of polluted environments. Bacteria were isolated from two cultivated plant root rhizopheric soil of Cocks comb (Celosia cristata) and Marigold (Tagetes erecta).The pesticide was tested at 5 elevated doses,0.5%, 1%, 2%, 2.5% and control. Physiochemical properties of soils, PH, Electrical conductivity, Organic carbon, Organic matter, Nitrogen, phosphorus, potassium, Manganese and Iron were analyzed. Based on morphological and biochemical tests the isolates were identified as Bacillus subtilis, Pseudomonas putida, Pseudomonas aeruginosa. Thus, the organisms can be exploited for bioremediation of chlorpyrifos polluted soil, and their ability to degrade other organophosphates pesticide.
In the years 1996-1997 the experiments were carried out on methods to investigate seed vigour of tassel flower (Amaranthus caudatus L.), sand pink (Dianthus chinensis L.), babies' breath (Gypsophila elegans M.R), sweet pea (Lathyrus odorathus L.), African marigold (Tagetes erecta L.) and zinnia (Zinnia elegans Jasq.). The main goals of this research were to specify conditions for accelerated ageing (AA) of the seeds of a few selected ornamental plant species and to choose the most appropriate methods for their seed vigour evaluation in the laboratory and greenhouse conditions. All used in the experiments seeds came from the commercial seed lots from Polish seed company. Evaluation was carried out on the seed samples with high and low vigour. The latter ones were received through subjecting the seed samples to AA, i.e. by placing them in 100% relative humidity (RH) at 44°C, except African marigold - at 42°C, in the darkness and keeping them for 144, 88, 100,48,72 and 72 hours, respectively. The tested seed vigour estimated methods included the Germ' s method, the 2,3,5-triphenyl tetrazoilum chloride (TTC) method and the test of plant emergences in the greenhouse. The high vigour seeds sampies were used as a check. The Germ' s method was found to be useful to evaluate sand pink, babies' breath and African marigold seed vigour, whereas the TTC method was found to be suitable for vigour evaluation of sand pink, babies' breath and zinnia. At present stage of our knowledge about seed vigour, the plant emergences in the greenhouse method was found to be the best for evaluation of seed vigour of tassel flower, sand pink, babies' breath, sweet pea and zinnia. It is reasonable to combine a few methods of seed vigour evaluation for ornamental plant species.
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